A5065772439- Sulfur Compounds in Biology
- Eicosanoids and Hypertension Pharmacology
- Nitric Oxide and Endothelin Effects
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Renin-Angiotensin System Studies
- Neuroscience of respiration and sleep
- Blood Coagulation and Thrombosis Mechanisms
- Sphingolipid Metabolism and Signaling
- Microbial Natural Products and Biosynthesis
- Mangiferin and Mango Extracts
- Marine Sponges and Natural Products
- Inflammatory mediators and NSAID effects
- Mast cells and histamine
- Adipokines, Inflammation, and Metabolic Diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Birth, Development, and Health
- Liver Disease Diagnosis and Treatment
- Traditional and Medicinal Uses of Annonaceae
- Ion channel regulation and function
- Drug Transport and Resistance Mechanisms
- Natural product bioactivities and synthesis
- Peroxisome Proliferator-Activated Receptors
- Inflammasome and immune disorders
University of Naples Federico II
2016-2025
Federico II University Hospital
2020
University of Patras
2010-2011
University Hospital, Newark
2011
The University of Texas Medical Branch at Galveston
2011
National and Kapodistrian University of Athens
2010
Recent studies have demonstrated that hydrogen sulfide (H(2)S) is produced within the vessel wall from L-cysteine regulating several aspects of vascular homeostasis. H(2)S generated cystathione γ-lyase (CSE) contributes to tone; however, molecular mechanisms underlying vasorelaxing effects are still under investigation.Using isolated aortic rings, we observed addition led a concentration-dependent relaxation was prevented by CSE inhibitors DL-propargylglyicine (PAG) and β-cyano-l-alanine...
Hydrogen sulphide (H2S) has been involved in cardiovascular homoeostasis but data about its role animal models of diabetic pathology are still lacking. Here, we have analysed H2S signalling a genetic model diabetes, the non-obese (NOD) mice.NOD mice exhibit progressive endothelial dysfunction characterized by reduced reactivity blood vessels as diabetes develops. NOD were divided into three groups according to different glycosuria values: I, II and III. Age-matched resistant (NOR) used...
A growing body of evidence suggests that hydrogen sulfide (H2S) is a signaling molecule in mammalian cells. In the cardiovascular system, H2S enhances vasodilation and angiogenesis. H2S-induced hypothesized to occur through ATP-sensitive potassium channels (KATP); however, we recently demonstrated it also increases cGMP levels tissues. Herein, studied involvement cGMP-dependent protein kinase-I vasorelaxation. The effect on vessel tone was phenylephrine-contracted aortic rings with or...
Therapeutic use of sulfhydrylated inhibitor S-zofenopril has raised different hypotheses regarding the role played by its thiol group in beneficial clinical effects exerted compared with other angiotensin-converting enzyme (ACE) inhibitors. Here, we investigated hydrogen sulfide (H2S) pathway as accountable for extra-beneficial vascular function. Spontaneously hypertensive rat (SHRs) and control Wistar Kyoto (WKY) rats were treated either or enalapril vivo. Aorta carotid harvested ex vivo...
While patients with nonalcoholic fatty liver disease (NAFLD) are at increased risk to develop clinically meaningful cardiovascular diseases (CVD), there no approved drug designed target the and CVD component of NAFLD. GPBAR1, also known as TGR5, is a G protein coupled receptor for secondary bile acids. In this study we have investigated effect GPBAR1 activation by BAR501, selective agonist, in Apolipoprotein E deficient (ApoE-/-) mice fed high fat diet fructose (Western diet), validated...
Diabetes is associated with severe vascular complications involving the impairment of endothelial nitric oxide synthase (eNOS) as well cystathionine γ-lyase (CSE) activity. eNOS function suppressed in hyperglycaemic conditions, resulting reduced NO bioavailability, which paralleled by levels hydrogen sulfide (H2S). Here we have addressed molecular basis interplay between and CSE pathways. We tested impact H2S replacement using mitochondrial-targeted donor AP123 isolated vessels cultured...
Sphingosine-1-phosphate (S1P) has been shown to regulate numerous and diverse cell functions, including smooth muscle contraction. Here we assessed the role of S1P/Sphingosine kinase (SPK) pathway in regulation bronchial tone. Our objective was determine, using an integrated pharmacologic molecular approach, (1) S1P as endogenous modulator tone, (2) linkage between hyperresponsiveness. We evaluated effects on isolated bronchi whole lungs, harvested from Balb/c mice sensitized ovalbumin (OVA)...
Two unprecedented cyclic peptides, solomonamides A and B, were isolated from the marine sponge Theonella swinhoei. The structures elucidated on basis of comprehensive 1D 2D NMR analysis high-resolution mass spectrometry. combined approach, involving Marfey's method, QM J based analysis, DFT J/(13)C calculations, was used for establishing absolute configuration entire molecule. Solomonamide showed in vivo anti-inflammatory activity.
Hydrogen sulfide (H<sub>2</sub>S), a novel gaseous transmitter, is considered physiological regulator of vascular homeostasis. Recent evidence suggests H<sub>2</sub>S as an endothelium-hyperpolarizing factor (EDHF) candidate. To address this issue, we evaluated the effect sodium hydrogen (NaHS), donor, on rat mesenteric arterial bed. NaHS concentration-response curve was performed preconstricted assess contribution EDHF, pharmacologic dissection using indomethacin,...
Nonalcoholic steatohepatitis (NASH) is associated with an increased risk of developing cardiovascular complications and mortality, suggesting that treatment NASH might benefit from combined approaches target the liver components NASH. Using genetic pharmacologic approaches, we show G protein-coupled bile acid-activated receptor 1 (GPBAR1) agonism reverses vascular damage in mouse models accelerated inflammation representing independent factor for development diseases cardiovascular-related...
Abstract In this paper, we report studies concerning thrombin binding aptamer (TBA) dimeric derivatives in which the 3′-ends of two TBA sequences have been joined by means linkers containing adenosine or thymidine residues and/or a glycerol moiety. CD and electrophoretic investigations indicate that all modified aptamers are able to form G-quadruplex domains resembling parent structure. However, isothermal titration calorimetry measurements aptamer/thrombin interaction point different...
Alzheimer's disease (AD) is one of the most prevalent forms neurodegenerative disorders. Previously, we have shown that in vivo administration an IL-17 neutralizing antibody (IL-17Ab) rescues amyloid‐β‐induced neuro-inflammation and memory impairment, demonstrating pivotal role AD-derived cognitive deficit. Recently, AD has been recognized as a more intriguing pathology affecting vascular networks platelet function. However, not much known about peripheral inflammation how pro-inflammatory...
Objectives Interleukin (IL) 17s cytokines are key drivers of inflammation that functionally dysregulated in several human immune-mediated inflammatory diseases (IMIDs), such as rheumatoid arthritis (RA), psoriasis and bowel disease (IBD). Targeting these has some therapeutic benefits, but issues associated with low efficacy immunogenicity for subgroups patients or IMIDs reduce their clinical use. Therefore, there is an urgent need to improve the coverage antibodies targeting IL-17A and/or...
Many antiproliferative G-quadruplexes (G4s) arise from the folding of GT-rich strands. Among these, Thrombin Binding Aptamer (TBA), as a rare example, adopts monomolecular well-defined G4 structure. Nevertheless, potential anticancer properties TBA are severely hampered by its anticoagulant action and, consequently, no related studies have appeared so far in literature. We wish to report here that suitable chemical modifications sequence can preserve over activity. Particularly, we replaced...
Here we report investigations, based on circular dichroism, nuclear magnetic resonance spectroscopy, molecular modelling, differential scanning calorimetry and prothrombin time assay, analogues of the thrombin binding aptamer (TBA) in which individual thymidines were replaced by 5-fluoro-2'-deoxyuridine residues. The whole data clearly indicate that all derivatives are able to fold a G-quadruplex structure very similar 'chair-like' conformation typical TBA. However, only ODNs TBA-F4:...
Here, by using in vitro and ex vivo approaches, we elucidate the impairment of hydrogen sulfide (H2S) pathway vascular complications associated with metabolic syndrome (MetS). In model simulating hyperlipidemic/hyperglycemic conditions, observe significant hallmarks endothelial dysfunction, including eNOS/NO signaling impairment, ROS overproduction, a reduction CSE-derived H2S. Transitioning to an db/db mice, genetic MetS model, identify downregulation CBS CSE expression aorta, coupled...