A5031528321- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Cardiovascular Disease and Adiposity
- Immune cells in cancer
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- IL-33, ST2, and ILC Pathways
- Polymer Surface Interaction Studies
- Omental and Epiploic Conditions
- Immune Cell Function and Interaction
- Endometriosis Research and Treatment
- Skin and Cellular Biology Research
- Animal Genetics and Reproduction
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Cutaneous lymphoproliferative disorders research
- Lipid metabolism and disorders
- Analytical Chemistry and Sensors
- CRISPR and Genetic Engineering
Leipzig University
2017-2024
Martin Luther University Halle-Wittenberg
2019-2021
Abstract A chronic low-grade inflammation within adipose tissue (AT) seems to be the link between obesity and some of its associated diseases. One hallmark this AT is accumulation macrophages (ATMs) around dead or dying adipocytes, forming so-called crown-like structures (CLS). To investigate dynamics CLS their direct impact on activation state ATMs, we established a laser injury model deplete individual adipocytes in living from double reporter mice (GFP-labeled ATMs tdTomato-labeled...
Background & AimsNon-alcoholic fatty liver disease (NAFLD) is reaching epidemic proportions, fueled by the obesity pandemic. In NAFLD, monocytes infiltrate visceral adipose tissue that promote local and hepatic inflammation. It however remains unclear what drives inflammation how immune landscape in differs across NAFLD severity spectrum. We aimed to assimilate macrophage (ATM) heterogeneity a cohort.MethodsVisceral macrophages from lean obese patients stratified into phenotypes underwent...
Obesity is associated with chronic low-grade inflammation of adipose tissue (AT) and an increase AT macrophages (ATMs) that linked to the onset type 2 diabetes. We have recently shown neutralization interleukin (IL)-6 in obese organ cultures inhibits proliferation ATMs, which occurs preferentially alternatively activated macrophage phenotype. In this study, we investigated biology metabolic phenotype mice myeloid cell-specific IL-6Rα deficiency (Il6ra Δmyel) after normal chow 20 weeks...
Human white adipose tissue (AT) is a metabolically active organ with distinct depot-specific functions. Despite their locations close to the gastrointestinal tract, mesenteric AT and epiploic (epiAT) have only scarcely been investigated. Here, we aim characterise these ATs in-depth estimate contribution alterations in whole-body metabolism.Mesenteric, epiploic, omental abdominal subcutaneous were collected from 70 patients obesity undergoing Roux-en-Y gastric bypass surgery. The...
White adipose tissue (WAT) fibrosis, characterized by an excess of extracellular (ECM) matrix components, is strongly associated with WAT inflammation and dysfunction due to obesity. Interleukin (IL)-13 IL-4 were recently identified as critical mediators in the pathogenesis fibrotic diseases. However, their role fibrosis still ill-defined. We therefore established ex vivo organotypic culture system demonstrated upregulation fibrosis-related genes increase α-smooth muscle actin (αSMA)...
Obesity is associated with chronic low-grade inflammation of visceral adipose tissue (AT) characterized by an increasing number AT macrophages (ATMs) and linked to type 2 diabetes. histologically indicated the formation so-called crown-like structures, as ATMs accumulate around dying adipocytes, occurrence multinucleated giant cells (MGCs). However, date, function MGCs in obesity unknown. Therefore, aim this study was characterize unravel these cells. We demonstrated that occurred obese...
Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is major interest. Our aim was study specificity most-common Cre/loxP approach for inducible gene manipulation AT in mice (AdipoqCre-ERT2). We used with tamoxifen-sensitive Cre recombinase controlled by adiponectin promoter (AdipoqCre-ERT2), which were crossed a tdTomato reporter mouse visualize site recombination on single-cell resolution. Albeit tamoxifen induced expression this model, also non-stimulated...
Adhesive processes in aqueous media play a crucial role nature and are important for many technological processes. However, direct quantification of adhesion still requires expensive instrumentation while their sample throughput is rather small. Here we present fast, easily applicable method on quantifying energy water based interferometric measurement polymer microgel contact areas with functionalized glass slides evaluation via the Johnson⁻Kendall⁻Roberts (JKR) model. The advantage that...
Obesity is concurrent with immunological dysregulation, resulting in chronic low-grade inflammation and cellular dysfunction. In pancreatic islets, this loss of function has been correlated mature β-cells dedifferentiating into a precursor-like state through constant exposure to inflammatory stressors. As adipocytes likewise have the capability dedifferentiate vitro vivo, we wanted analyze change relation adipose tissue (AT) macrophage (ATM) activity. Using our organotypic AT explant culture...
Abstract Obesity is frequently associated with a chronic low‐grade inflammation in the adipose tissue (AT) and impaired glucose homeostasis. Adipose macrophages (ATMs) have been shown to accumulate inflamed AT either by means of recruitment from blood or local proliferation. ATM proliferation activation can be stimulated TH2 cytokines, such as IL‐4 IL‐13, suggesting involvement CD4‐positive T cells activation. Furthermore, several studies alterations metabolism. Therefore, we sought examine...
Obesity is associated with a chronic low-grade inflammation in visceral adipose tissue (AT) characterized by an increasing number of macrophages (ATMs) and linked to type 2 diabetes. AT histologically indicated the formation so-called crown-like structures (CLS), as accumulation ATMs around dying adipocytes, occurrence multi-nucleated giant cells (MGCs). However date, function MGCs obesity unknown. Hence, aim this study was characterize unravel these cells. <p>We demonstrate that occur...
Obesity is associated with a chronic low-grade inflammation in visceral adipose tissue (AT) characterized by an increasing number of macrophages (ATMs) and linked to type 2 diabetes. AT histologically indicated the formation so-called crown-like structures (CLS), as accumulation ATMs around dying adipocytes, occurrence multi-nucleated giant cells (MGCs). However date, function MGCs obesity unknown. Hence, aim this study was characterize unravel these cells. <p>We demonstrate that occur...