A5010938612- Diet, Metabolism, and Disease
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Peptidase Inhibition and Analysis
- Heart Rate Variability and Autonomic Control
- Diabetes Management and Research
- Diabetes and associated disorders
- Nitric Oxide and Endothelin Effects
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Cardiovascular Function and Risk Factors
- Cardiovascular and exercise physiology
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Eicosanoids and Hypertension Pharmacology
- Dietetics, Nutrition, and Education
- Human Health and Disease
- Natural Antidiabetic Agents Studies
Universidade Nova de Lisboa
2011-2024
Instituto Politécnico de Lisboa
2023
Instituto Gulbenkian de Ciência
2015-2021
Nova Medical (United States)
2005-2021
University of Lisbon
2007-2008
Portuguese Environment Agency
2008
University of Manitoba
2008
Direção Geral do Património Cultural
2006
Escola Superior de Saúde Egas Moniz
2001
Insulin resistance contributes to the pathophysiology of diabetes and is a hallmark obesity, metabolic syndrome, many cardiovascular diseases. Therefore, quantifying insulin sensitivity/resistance in humans animal models great importance. Various methods are used assess sensitivity both individuals study populations. Validity, reproducibility, cost, degree subject burden important factors for clinicians researchers consider when weighing merits particular method. Some rely on steady-state...
Reduced plasma nitrate (NOx) levels and increased urinary norepinephrine (U-NE) have been described in severe obstructive sleep apnea (OSA), are reverted by continuous positive airway pressure (CPAP). The effect of CPAP on these biomarkers mild-moderate OSA is not well understood. aim this study was to compare NOx U-NE blood (BP) between male patients with determine the impact 1 month therapy parameters. We undertook a prospective 67 consecutive (36 mild-moderate, 31 severe). Measurements at...
The hepatic parasympathetic system is one of the major contributors for preserving insulin sensitivity in postprandial state. Postprandial vagal control whole-body glucose clearance and its effect on specific organs remains unknown. Our hypothesis that, state, nerves (HPN) are responsible a considerable part extra-hepatic tissue clearance. Two groups 9-week-old Sprague–Dawley rats were studied, comparing sham-operated versus denervated animals. Insulin was evaluated state by rapid test...
Coffee may protect against non-alcoholic fatty liver disease (NAFLD), but the roles of caffeine and non-caffeine components are unclear. intake by 156 overweight subjects (87% with Type-2-Diabetes, T2D) was assessed via a questionnaire, 98 (all also providing 24 h urine sample for quantification coffee metabolites LC–MS/MS. NAFLD characterized index (FLI) Fibroscan® assessment fibrosis. No associations were found between self-reported parameters; however, total metabolites, defined as...
In animal studies, the whole-body glucose disposal effect of insulin is low in fasted state or after atropine infusion, but doubles a meal, consistent with hepatic insulin-sensitizing substance (HISS) hypothesis. We tested how standardized test meal and affected dynamic response to humans. Insulin sensitivity was assessed healthy male subjects (aged 28.9 ± 1.9 years, body mass index 23.3 0.8 kg·m –2 ) by using rapid (RIST), which transient euglycemic clamp. After 24-hour fasting period, then...
Background:Carvedilol is known to be an adrenoreceptor blocker and free radical scavenger, used in hypertension cardiac failure. However, its therapeutic actions cannot fully explained by these mechanisms. In studies, we tested the hypothesis that carvedilol action associated with synthesis/release of nitric oxide (NO).
Introduction: Nutrition has been underrepresented in the curriculum of many medical schools and therefore physicians do not feel adequately prepared to provide dietary counselling. The aim present study is determine impact a Metabolism curricular unit on nutrition attitudes, knowledge confidence future clinical practice students.Material Methods: All students enrolled (2017/2018) were invited complete questionnaire assessing their nutritional eating habits at beginning end semester (n =...
Type 2 diabetes (T2D) heterogeneity is a major determinant of complications risk and treatment response. Using cluster analysis, we aimed to stratify glycemia within metabolic multidimensionality extract pathophysiological insights out profiling. We performed analysis 974 subjects (PREVADIAB2 cohort) with normoglycemia, prediabetes, or non-treated diabetes. The algorithm was informed by age, anthropometry, milieu (glucose, insulin, C-peptide, free fatty acid (FFA) levels during the oral...
Glucagon levels are often moderately elevated in diabetes. It is known that glucagon leads to a decrease hepatic glutathione (GSH) synthesis turn associated with decreased postprandial insulin sensitivity. Given cAMP pathway controls GSH we tested whether sensitivity decreases after intraportal (ipv) administration of analog (DBcAMP), and investigated promotes resistance through decreasing levels.Insulin was determined fed male Sprague-Dawley rats using modified euglycemic hyperinsulinemic...
The dynamic response to insulin is highly potentiated after meal ingestion, and this meal-induced sensitization (MIS) in healthy subjects dependent on cholinergic mechanisms. main objective of study was test the hypothesis that reduced observed moderately overweight subjects, comparison with control lean due MIS impairment not a reduction direct hypoglycemic action insulin. Both male were recruited. Insulin sensitivity (IS) assessed by rapid (RIST) performed 24 h fast, as well standardized...
Abstract Aims/hypothesis Imbalances in glucose metabolism are hallmarks of clinically silent prediabetes (defined as impaired fasting and/or tolerance) representing dysmetabolism trajectories leading to type 2 diabetes. CD26/dipeptidyl peptidase 4 (DPP4) is a proven molecular target diabetes-controlling drugs but the DPP4 gene control dysglycaemia not proven. Methods We dissected genetic post-OGTT and insulin release responses by Portuguese population-based cohort mainly European ancestry...
Whole-body insulin sensitivity (IS) depends on a hepatic pathway, involving parasympathetic activation and nitric oxide (NO) production. Both atropine N-monomethyl-L-arginine (L-NMMA, NO synthase inhibitor) induce resistance (IR). IR is associated with obesity. Because action was shown to be impaired in animal models of obesity, such as the obese Zucker rat (OZR), we tested hypothesis that hepatic-dependent pathway diminished OZR, resulting IR.Lean rats (LZRs) were used OZR controls. IS...
The Zucker diabetic fatty (ZDF) rat is an obesity and type 2 diabetes model. Progression to well characterised in ZDF rats, but only the fasted state. We evaluated mechanisms underlying postprandial insulin resistance young rats. tested hypothesis that overall action of affected rats as a result impairment hepatic parasympathetic-nitric oxide (PSN-NO) axis and/or glutathione (GSH), resulting decreased indirect (PSN-NO axis) direct actions insulin. Nine-week-old male lean (LZR, controls) were...
The liver is a fundamental organ to ensure whole-body homeostasis, allowing for proper increase in insulin sensitivity from the fast postprandial status. Hepatic regulation of glucose metabolism crucial and has been shown be modulated by glutathione (GSH) nitric oxide (NO). However, knowledge metabolic action GSH NO homeostasis remains incomplete. current study was designed test hypothesis that treatment with S-nitrosoglutathione sufficient revert resistance induced high-sucrose diet. Male...
Abstract Background Type 2 diabetes (T2DM) and obesity are characterized by altered insulin metabolism action. Reduced hepatic clearance is increasingly recognized as a key contributor to hyperinsulinemia resistance. CEACAM1 promotes clearance, its loss in hepatocytes associated with reduced mice men. This study examines whether circulating levels reflect compromised resistance the PREVADIAB2 cohort. Methods A total of 1019 individuals from cohort were evaluated for 75 g‐OGTT classified...
The physiological roles of insulin and nitric oxide (NO) have recently been recognized by several studies. A diversity chemical modifications is reported both in vivo vitro. S-nitrosation, the covalent linkage NO to cysteine-free thiol, as an important post-translational regulation many proteins. Here, we report vitro synthesis a S-nitrosothiol bovine A- B-chains. These compounds were characterized their HPLC chromatographic behavior, monitored UV visible spectroscopy electron spray...