A5070342040- Allergic Rhinitis and Sensitization
- Mast cells and histamine
- Asthma and respiratory diseases
- Head and Neck Surgical Oncology
- Olfactory and Sensory Function Studies
- Dermatology and Skin Diseases
- Food Allergy and Anaphylaxis Research
- Sinusitis and nasal conditions
- Head and Neck Cancer Studies
- Dysphagia Assessment and Management
- Polyamine Metabolism and Applications
- Oral and Maxillofacial Pathology
- Biochemical Analysis and Sensing Techniques
- IL-33, ST2, and ILC Pathways
- Respiratory and Cough-Related Research
- Tumors and Oncological Cases
- Tendon Structure and Treatment
- Ear Surgery and Otitis Media
- Sports injuries and prevention
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Advanced Chemical Sensor Technologies
- Facial Nerve Paralysis Treatment and Research
- Receptor Mechanisms and Signaling
- Vestibular and auditory disorders
- Urticaria and Related Conditions
Tokushima University
2016-2025
Institute of Biomedical Science
2016-2024
Tokushima University Hospital
2012-2023
Tottori University
2016
Fukuyama Cardiovascular Hospital
2016
National Hospital Organization
2016
Kagoshima University
1996-2015
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2010-2013
National Institutes of Health
2013
Neuroscience Institute
2011
Sphingosine kinase 1 (SphK1) and SphK2 are ubiquitous enzymes that generate sphingosine-1-phosphate (S1P), a ligand for family of G protein-coupled receptors (S1PR1-S1PR5) with important functions in the vascular immune systems. Here we explore role these kinases recovery from anaphylaxis mice. We found Sphk2-/- mice had rapid anaphylaxis. In contrast, Sphk1-/- showed poor delayed histamine clearance. Injection S1P into increased clearance promoted Adoptive cell transfer experiments...
The histamine H<sub>1</sub> receptor (H1R) gene is up-regulated in patients with allergic rhinitis. However, the mechanism and reason underlying this up-regulation are still unknown. Recently, we reported that H1R expression level strongly correlated severity of symptoms. Therefore, understanding will help to develop new anti-allergic drugs targeted for expression. Here studied molecular HeLa cells express endogenously response phorbol 12-myristate 13-acetate (PMA). In cells, stimulation...
Objectives/Hypothesis We aimed to test the hypothesis that chemotherapy changes gene expression of taste receptors in tongue induce dysgeusia patients with head and neck cancer. Study Design Prospective observation study. Methods enrolled 21 who received chemoradiotherapy five underwent radiotherapy for The messenger RNA (mRNA) levels receptor subunits T1R1, T1R2, T1R3, T2R5 were measured lingual mucosa scrapings obtained a small spatula. perception thresholds umami, sweet, bitter tastes...
Abstract Kujin contains antiallergic compounds that inhibit upregulation of histamine H 1 receptor (H1R) and interleukin ( IL )‐4 gene expression. However, the underlying mechanism remains unknown. We sought to identify a Kujin‐derived compound investigate its action. The H1R ‐4 mRNA levels were determined by real‐time quantitative RT ‐ PCR . To effects maackiain in vivo, toluene‐2,4‐diisocyanate TDI )‐sensitized rats used as nasal hypersensitivity animal model. identified (−)‐maackiain...
Histamine is a major chemical mediator in the development of nasal allergy, which characterized by hypersensitivity. In this study, we used rats sensitized exposure to toluene diisocyanate (TDI) as an animal model hypersensitivity and examined changes expression histamine H1 receptor (H1R) mucosa. The effect glucocorticoid on upregulation H1R mucosa induced TDI was also examined.In TDI, allergy-like behavior scored during 10-min period after provocation. determined means real-time...
The significant correlation between nasal symptom scores and level of histamine H1 receptor (H1R) mRNA in mucosa was observed patients with pollinosis, suggesting that H1R gene is an allergic disease sensitive gene. We demonstrated interleukin (IL)-9 are the rhinitis (AR)-sensitive genes protein kinase Cδ (PKCδ) signaling nuclear factor activated T-cells (NFAT) involved their expressions, respectively. Honey bee products have been used to treat diseases. However, pathological mechanism...
Allergic rhinitis (AR) is an inflammatory disorder typified by symptoms such as sneezing, congestion, and rhinorrhea. Histamine plays important roles in eliciting AR symptoms. Up-regulation of the histamine H(1) receptor (H1R) histidine decarboxylase (HDC) mRNAs was observed patients. Th2 cytokines are also involved pathogenesis AR. We examined effect suplatast tosilate on nasal symptoms, H1R, HDC, IL-4 gene expression using toluene-2,4-diisocyanate (TDI)-sensitized rats HeLa cells...
The therapeutic use of Kampo medicine, Sho-seiryu-to (SST) in allergic disorders is well known. As histamine plays a central role diseases, it possible that SST affects the allergy-related signaling. In this study, we investigated effect on signaling nasal mucosa toluene 2, 4-diisocyanate (TDI)-sensitized allergy model rats. Six-week-old male, Brown Norway rats were sensitized for 2 weeks with 10 μl 10% TDI, and after 1 week interval, provocation was initiated same amount TDI. (0.6 g/rat)...
We determined whether the expression of VEGF-A and -C their receptors, Flt-1 Flt-4, are associated with primary tumor size, regional lymph node metastasis, distant metastasis prognosis in patients tongue carcinoma. The -C, biopsy specimens taken from 73 carcinoma were examined by immunohistochemical staining. was failure VEGF-C correlated locoregional recurrence failure. Furthermore, N0 cases. Multivariate analysis revealed that an exclusively independent factor influencing metastasis. In...
Histamine H(1) receptor (H1R) gene is up-regulated in patients with allergic rhinitis, and its expression level strongly correlates the severity of symptoms. However, mechanism underlying this remains unknown. Here we report H1R up-regulation. The luciferase assay revealed existence two promoter regions, A B1. Two AP-1 one Ets-1 bound to region A, while Ku86, Ku70, PARP-1 Ku86 was responsible for DNA binding poly(ADP-ribosyl)ated response phorbol-12-myristate-13-acetate stimulation, inducing...
Conclusions: These findings suggest that the down-regulation of interleukin (IL)-5 gene expression in collaboration with suppression histamine H1 receptor (H1R) nasal mucosa provides basis for better therapeutic effects preseasonal prophylactic treatment antihistamines patients seasonal allergic rhinitis caused by Japanese cedar pollen. Objectives: The administration on IL-5 and IL-33 mRNA pollinosis were investigated. Methods: Eight had already visited hospital before peak pollen period...
In the present study, we examined effects of antihistamine on up-regulation H1R mRNA in nasal mucosa patients with pollinosis induced by controlled exposure to pollen using an environmental unit. Out 20 patients, designated 14 responders, whose levels were increased after first and excluded 6 non-responders. Accordingly, without treatment significantly both symptoms responders. Subsequently, prophylactic administration prior second inhibited above Moreover, expression before responders...
Histamine synthesized by histidine decarboxylase (HDC) from L-histidine is a major chemical mediator in the development of nasal allergy which characterized hypersensitivity. However regulatory mechanism histamine synthesis HDC remains to be elucidated. The objectives present study were examine changes content, activity and mRNA expression mucosa model rats sensitized exposure toluene diisocyanate (TDI) investigate effect dexamethasone on above mentioned allergic parameters. Rats provocated...
In the present study, an attempt was made to examine effects of aural stimulation with ointment containing capsaicin on swallowing function in order develop a novel and safe treatment for non-obstructive dysphagia elderly patients.A prospective pilot, non-blinded, non-controlled study case series evaluating new treatment.Secondary hospitals.The included 26 patients dysphagia. Ointment 0.025% (0.5 g) applied external auditory canal cotton swab under otoscope only once or day 7 days before...