A5089867564- Inflammatory mediators and NSAID effects
- Estrogen and related hormone effects
- Receptor Mechanisms and Signaling
- Eicosanoids and Hypertension Pharmacology
- Protein Kinase Regulation and GTPase Signaling
- Neuropeptides and Animal Physiology
- Peroxisome Proliferator-Activated Receptors
- Cancer, Stress, Anesthesia, and Immune Response
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Sphingolipid Metabolism and Signaling
- Nitric Oxide and Endothelin Effects
- Asthma and respiratory diseases
- Steroid Chemistry and Biochemistry
- NF-κB Signaling Pathways
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cell Adhesion Molecules Research
- Allergic Rhinitis and Sensitization
- Angiogenesis and VEGF in Cancer
- Cancer, Lipids, and Metabolism
- Cancer Treatment and Pharmacology
- PI3K/AKT/mTOR signaling in cancer
- Alcohol Consumption and Health Effects
- Connexins and lens biology
- Renin-Angiotensin System Studies
Tokushima University
2015-2024
Pharmac
2021-2023
Institute of Biomedical Science
2018-2022
Chiba University
2007-2016
University of Arizona
1999-2005
Orthopedic Center
2000
Tohoku Medical and Pharmaceutical University
1997
Hokkaido University
1997
Sapporo University
1997
Recently we have shown that the FPB prostanoid receptor, a G-protein-coupled receptor couples to Gαq, activates T-cell factor (Tcf)/lymphoid enhancer (Lef)-mediated transcriptional activation (Fujino, H., and Regan, J. W. (2001) Biol. Chem. 276, 12489–12492). We now report EP2 EP4 receptors, which couple Gαs, also activate Tcf/Lef signaling. By using Tcf/Lef-responsive luciferase reporter gene, activity was stimulated ∼10-fold over basal by 1 h of treatment with prostaglandin E2 (PGE2) in...
Prostaglandin E<sub>2</sub>(PGE<sub>2</sub>) mediates its physiological effects by interactions with a subfamily of G-protein-coupled receptors known as EP receptors. These consist four primary subtypes named EP<sub>1</sub>, EP<sub>2</sub>, EP<sub>3</sub>, and EP<sub>4</sub>. The EP<sub>2</sub> EP<sub>4</sub> are to couple Gα<sub>s</sub> stimulate intracellular cyclic 3,5- adenosine monophosphate formation, whereas the EP<sub>1</sub> EP<sub>3</sub> Gα<sub>q</sub> Gα<sub>i</sub>,...
Abstract Iron (Fe) is an essential nutrient, but poorly bioavailable because of its low solubility in alkaline soils; this leads to reduced agricultural productivity. To overcome problem, we first showed that the soil application synthetic 2′-deoxymugineic acid, a natural phytosiderophore from Poaceae, can recover Fe deficiency rice grown calcareous soil. However, high cost and poor stability acid preclude use. In work, develop more stable less expensive analog, proline-2′-deoxymugineic...
The EP<sub>2</sub> and EP<sub>4</sub> prostanoid receptors are G-protein-coupled whose activation by their endogenous ligand, prostaglandin (PG) E<sub>2</sub>, stimulates the formation of intracellular cAMP. We have previously reported that stimulation cAMP in EP<sub>4</sub>-expressing cells is significantly less than EP<sub>2</sub>-expressing cells, despite nearly identical levels receptor expression (<i>J Biol Chem</i><b>277:</b>2614–2619, 2002). In addition, a component signaling, but not...
The EP<sub>2</sub> and EP<sub>4</sub> prostanoid receptor subtypes are G-protein-coupled receptors for prostaglandin E<sub>2</sub> (PGE<sub>2</sub>). Both known to couple the stimulatory guanine nucleotide binding protein (Gα<sub>s</sub>) and, after stimulation with PGE<sub>2</sub>, can increase formation of intracellular cAMP. In addition, PGE<sub>2</sub> activate phosphatidylinositol 3-kinase (PI3K) leading phosphorylation extracellular signal-regulated kinases (ERKs) induction early...
Prostaglandin F<sub>2α</sub>(PGF<sub>2α</sub>) exerts its biological effects by binding to and activating FP prostanoid receptors. These receptors, which include two isoforms, the FP<sub>A</sub> FP<sub>B</sub>, have been cloned from a number of species are members superfamily G-protein-coupled Previous studies shown that activation receptors leads phosphatidylinositol hydrolysis, intracellular calcium release, protein kinase C. Here, we demonstrate PGF<sub>2α</sub> treatment 293-EBNA...
We examined the antitumour effect of a combination betulinic acid (BA) and vincristine (VCR) on murine melanoma B16F10 cells in vitro vivo. Betulinic acid, pentacyclic triterpene, showed synergistic cytotoxic by combinational use VCR. VCR induced cell cycle arrest at different points (BA G1 phase G2/M phase) caused apoptosis cells. In vivo study, inhibited metastasis tumour to lung. The addition BA augmented suppression experimental lung C57BL/6 mice. number nodules more than 1 mm diameter...
Abstract Background Transient receptor potential channel melastatin 8 ( TRPM 8) is activated by cold temperatures and cooling agents (menthol icilin). Recent studies showed expressed in visceral organs peripheral sensory pathways. However, the role of hyperalgesia poorly understood pathological states such as inflammatory bowel disease. Hence, we investigated distribution its involvement experimental colitis mice. Methods immunoreactivity was detected using immunohistochemical staining with...
Renal tubulointerstitial injury, an inflammation-associated condition, is a major cause of chronic kidney disease (CKD). Levels activated factor X (FXa), blood coagulation factor, are increased in various inflammatory diseases. Therefore, we investigated the protective effects FXa inhibitor against renal injury using unilateral ureteral obstruction (UUO) mice (a fibrosis model) and Food Drug Administration Adverse Events Reporting System (FAERS) database. The expression levels FX receptors...
FP prostanoid receptors are G-protein-coupled (GPCR) that consist of two known isoforms, FPA and FPB. These which generated by alternative mRNA splicing, identical except for their carboxyl-terminal domains. Previously we have shown stimulation both isoforms with prostaglandin F2α(PGF2α) activates the small G-protein Rho, leading to morphological changes consisting cell rounding formation aggregates. Following removal PGF2α, however, FPA-expressing cells show rapid reversal rounding, whereas...
A positive association between vascular endothelial growth factor-C (VEGF-C) expression and lymph node metastasis has been reported in several cancers. However, the relationship of VEGF-C some cancers, including non-small cell lung cancer (NSCLC), is controversial. We evaluated factor receptor-3 (VEGFR-3) NSCLC samples from patients who had undergone surgery 1998 2002 using real-time quantitative RT-PCR immunohistochemical staining. failed to find a VEGFR-3 mRNA NSCLC. An immunohistological...
The significant correlation between nasal symptom scores and level of histamine H1 receptor (H1R) mRNA in mucosa was observed patients with pollinosis, suggesting that H1R gene is an allergic disease sensitive gene. We demonstrated interleukin (IL)-9 are the rhinitis (AR)-sensitive genes protein kinase Cδ (PKCδ) signaling nuclear factor activated T-cells (NFAT) involved their expressions, respectively. Honey bee products have been used to treat diseases. However, pathological mechanism...
Drug repositioning is a cost-effective method to identify novel disease indications for approved drugs; it requires shorter developmental period than conventional drug discovery methods. We aimed prophylactic drugs oxaliplatin-induced peripheral neuropathy by using data from large-scale medical information and life science databases.Herein, we analyzed the reported between 2007 2017 retrieved FDA's database of spontaneous adverse event reports (FAERS) LINCS provided National Institute...
It is known that there are some bidirectional interactions between the nervous and immune systems via neurotransmitters cytokines. To clarify whether any modulate lymphocyte functions, we examined effects of oxotremorine-M (Oxo-M) on interleukin-2 (IL-2) production in human peripheral blood lymphocytes by using enzyme-linked immunosorbent assays, Northern blot analyses, reverse transcriptase-polymerase chain reaction, fluorescence-activated cell sorter. Pretreatment cells with Oxo-M (10 nm...