To develop hydrogels with high mechanical strength for cell encapsulation and three-dimensional culture is a critical challenge cartilage tissue engineering. In this study, novel double network (DN) were fabricated phosphate buffer solution (PBS) was used as the solvent to facilitate encapsulation. The tough DN prepared through two-step photopolymerization using neutral oligo(2,2-dimethyltrimethylene carbonate)–poly(ethylene glycol)–oligo(2,2-dimethyltrimethylene carbonate)–diacrylate...

Various scaffolding systems have been attempted to facilitate vascularization in tissue engineering by optimizing biophysical properties (e.g., vascular-like structures, porous architectures, surface topographies) or loading biochemical factors growth factors, hormones). However, during ossification remains an unmet challenge that hampers the repair of large bone defects. In this study, reconstructing vascularized bones situ against critical-sized defects is endeavored using newly developed...

We introduce another type of Pearcey beam, namely, dual (DP) beams, based on the function catastrophe theory. DP beams are experimentally generated by applying Fresnel diffraction bright elliptic rings. Form-invariant Bessel distribution can be regarded as a special case beams. Subsequently, basic propagation characteristics identified. result interference two half instead classical Moreover, we also verified that (including special-case parabolic-like beams) elliptical rings (circular...

One bottleneck in tissue regeneration with hydrogel scaffolds is the limited understanding of crucial factors for controlling hydrogel's physical microenvironments to regulate cell fate. Here, effects permeability and living space hydrogels on encapsulated cells' behavior were evaluated, respectively. Three model hydrogel-based constructs are fabricated by using photo-crosslinkable hyaluronic acid as precursor chondrocytes type. The better permeable facilitate viability rapid proliferation,...

Dedifferentiation of chondrocyte greatly restricts its function and application, however, it is poorly understood except a small number canonical markers. The non-cell-adhesive property endows polysaccharide hydrogel with the ability to maintain phenotype, which can serve as platform identify new molecular markers therapeutic targets dedifferentiation. In this study, high-throughput RNA sequencing (RNA-seq) was first performed on articular chondrocytes at primary (P0) passage 1 (P1) stages...

Hydrogel scaffold is a popular cell delivery vehicle in tissue engineering and regenerative medicine due to its capability encapsulate cells as well modifiable properties. However, the inherent submicron- or nano-sized polymer networks of conventional hydrogel will produce spatial constraints on cellular activities encapsulated cells. In this study, we endeavor develop an innovative encapsulatable cryogel (CECG) platform with interconnected macro-pores, by combining cryopreservation...

Polydimethylsiloxane (PDMS) is a common biomaterial in the fabrication of microfluidic devices due to its various beneficial properties. However, inherent highly hydrophobic surfaces often limit PDMS-based for myocardial differentiation pluripotent stem cells. In order improve cell interactive properties PDMS, gelatin-dopamine was synthesized by grafting dopamine onto gelatin backbone. The conjugation verified using proton nuclear magnetic resonance spectroscopy (1H NMR). Compared with...

A new iterative method for creating a pure phase hologram to diffract light into two arbitrary two-dimensional intensity profiles in output planes is presented. This combines the Gerchberg-Saxton (GS) algorithm and compensation algorithm. Numerical simulation indicates that outperforms most frequently used accuracy when it generate large size images. preliminary experiment of optical reconstruction has been taken verify feasibility our method.

A robust methodology, APSC-8-oxoGua-seq, for the sequencing of 8-oxoGua in microRNA transcriptome at single-base resolution has been developed.

A series of biodegradable hydrogels based on oligo(2,2-dimethyltrimethylene carbonate)-block-poly(ethylene glycol)-block-oligo(2,2-dimethyltrimethylene carbonate) diacrylate (DPD-DA) precursor with varied length hydrophilic poly(ethylene glycol) (PEG) segment and hydrophobic (ODTC) were prepared by photopolymerization. Hydrophobic interaction was found to affect the properties hydrogel. The elastic modulus toughness hydrogel could be tuned altering lengths ODTC as well PEG segment. In a...

Based on Talbot effect of optical microgratings, we report an ultra-compact sensor for displacement and vibration measurement with resolution down to sub-nanometer level. With no need components such as reflectors, splitters, polarizers, wave plates, the proposed based a common-path structure shows high compactness. Using gratings period 3 µm, within range 1 mm is demonstrated experimentally. Associated interpolation circuit subdividing factor 4096, 0.73 nm obtained. The experimental results...

By using the diffractive optical elements written onto a spatial light modulator, we experimentally obtain regular triple-cusp beams. Their propagation characteristics and topological structures are subsequently investigated. The experimental results demonstrate that each cusp of an beam, similar to main lobe Airy propagates along curved paths in free space, hence tends adopt "transverse acceleration" property. Moreover, prove beams can resist local distorted deformation. Such thus be...

The transport of nutrients and oxygen by vascular networks into engineered tissue constructs is critical to their successful integration host tissues. Hydrogel has achieved some promising results as scaffolds for vascularization. However, the vascularization hydrogel still constrained its inherent submicron- or nano-sized pores. In this study, two gelatin-based micro-cavitary gel (Gel-MCG) with varying densities micro-cavities were developed a photocrosslinkable gelatin methacrylate (Gel-MA)...