A5075642180- Cytomegalovirus and herpesvirus research
- Cellular transport and secretion
- Immune Cell Function and Interaction
- Herpesvirus Infections and Treatments
- Toxoplasma gondii Research Studies
- Calcium signaling and nucleotide metabolism
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- Complement system in diseases
- Mosquito-borne diseases and control
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Higher Education Learning Practices
- HIV Research and Treatment
- Erythrocyte Function and Pathophysiology
- Cardiovascular and exercise physiology
- Immune Response and Inflammation
- Adenosine and Purinergic Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Caveolin-1 and cellular processes
- Retinal Development and Disorders
- Human Health and Disease
- Extracellular vesicles in disease
University of Rijeka
2014-2025
University North
2018-2025
Ludwig-Maximilians-Universität München
1998
Heidelberg University
1994-1995
Universität Ulm
1992-1995
Reactivation from latent cytomegalovirus (CMV) infection is often associated with conditions of immunosuppression and can result in fatal disease. Whether the maintenance systemic CMV latency mainly governed by factors infected cell or immune control functions unknown. Likewise, putative mechanisms which could prevent induction spread recurrent are not clearly identified. We took advantage latently B cell-deficient mice a sensitive method for virus detection to study reactivation after...
Virus shedding from the epithelial cells of serous acini salivary glands is a major source for horizontal transmission cytomegalovirus. These are, different to other tissues, exempt CD8 T lymphocyte control. CD4 lymphocytes are essential terminate productive infection. Here, we prove that T-B cooperation and production antibodies not required this process. For infection with murine cytomegalovirus, mutant mice were used which do produce because disrupted membrane exon immunoglobulin mu chain...
Cytomegalovirus (CMV), similar to other members of the Herpesviridae family, can establish both persistent and latent infections. Each CMVs that are found in many animal species replicates salivary gland, oral secretion represents a source horizontal transmission. Locally restricted replication characterizes immunocompetent individual, whereas immunocompromised host, protean disease manifestations occur due virus dissemination. The is cleared by immune surveillance, CD8+ T lymphocytes play...
Although the relative contribution of different immune effector functions to clearing tissues cytomegalovirus is controversial, CD8+ T lymphocytes has generally been accepted as essential. In this report, we show that under certain conditions T-lymphocyte subset can be dispensable for clearance cytomegalovirus. Mice depleted eliminated infectious virus with a kinetics similar normal mice. Adoptive transfer studies revealed limitation spread required cooperation between CD4+ and other cells....
Selective expression of murine cytomegalovirus (MCMV) immediate-early (IE) genes leads to the presentation by major histocompatibility complex (MHC) class I molecule Ld a peptide derived from MCMV IE protein pp89 (Reddehase, M.J., J. B. Rothbard, and U.H. Koszinowski. 1989. Nature (Lond.). 337:651). Characterization endogenous antigenic peptides identified as nonapeptide 168YPHFMPTNL176 (del Val, M., H.-J. Schlicht, T. Ruppert, M.J. Reddehase, 1991. Cell. 66:1145). Subsequent early prevents...
Cytomegalovirus (CMV) infection reorganizes early endosomes (EE), recycling endosome (RE), and trans-Golgi network (TGN) expands their intermediates into a large perinuclear structure that forms the inner part of cytoplasmic assembly complex (AC). The reorganization begins results with basic configuration (known as pre-AC) in (E) phase infection, but sequence developmental steps is not yet well understood. One first signs establishment pre-AC, which can be observed by immunofluorescence,...
We have shown previously that the antiviral function of CD4+ T lymphocytes against murine cytomegalovirus (MCMV) is associated with release interferon- (IFN-γ). now demonstrate IFN-γ and tumour necrosis factor alpha (TNF-α) display synergism in their activity. As little as 2 ng/ml TNF-α reduced virus yield by about three orders magnitude. There was no effect on immediate early (IE) (E) gene expression far candidate genes IE1, El those encoding major DNA- binding protein DNA polymerase were...
An immediate-early protein of murine cytomegalovirus (MCMV), pp89, elicits an immunodominant and protective major histocompatibility complex (MHC) class I Ld-restricted CD8+ T-lymphocyte response. Remarkably, presentation the naturally processed peptide nonapeptide YPHFMPTNL, is abolished during permissive MCMV infection in vitro. This defect pp89 due to expression early gene functions that specifically block transport peptide-charged MHC complexes cell surface (M. Del Val, H. Hengel,...
Interferon gamma (IFN gamma) represents an essential cytokine involved in murine cytomegalovirus (MCMV) clearance from the salivary gland and control of horizontal transmission. Because IFN cannot be responsible for all effects during recovery MCMV infection we have now tested potential participation tumour necrosis factor alpha (TNF alpha) antiviral defence. Neutralization endogenous TNF abolished activity CD4 T cells immunocompetent as well CD8 subset-deficient mice. These data suggest...
Mouse cytomegalovirus (MCMV) functions expressed at the beginning of early phase viral replication cycle interfere with major histocompatibility complex (MHC) class I-restricted pathway antigen presentation (M. J. Reddehase, M. R. Fibi, G. Keil, and U. H. Koszinowski, Virol. 60:1125-1129, 1986; Del Val, K. Münch, Cell 58:305-315, 1989). Nascent MHC I heavy chains associate beta 2-microglobulin peptide, but assembled trimolecular is retained in endoplasmatic reticulum/cis-Golgi compartment...
Several herpesviruses, including cytomegalovirus, induce receptors for the Fc domain of murine immunoglobulin G (IgG) molecules. Viral genes coding these have been characterized only alphaherpesviruses. In this report, we describe a new approach that led to identification an receptor (FcR) cytomegalovirus (MCMV). The fragment IgG precipitated glycoproteins (gp) 86 88 and 105 kDa from MCMV-infected cells. Deglycosylation by endoglycosidase F resulted in protein with molecular mass 64 kDa....
Infections are leading causes of increased morbidity and mortality severe traumatic brain-injured (STBI) patients. The mechanism underlying the susceptibility to infections is still unexplained. purpose study was investigate changes in frequency leucocytes subpopulations peripheral blood patients with STBI during course intensive care treatment. Twenty were included study. Healthy age- sex- volunteers served as control. Peripheral samples taken from these at day 1, 4 7, mononuclear cells...
Background/Objectives: Cytomegalovirus (CMV) infection expands early endosomes (EEs) into tubular extensions that may contribute to the control of virus replication and virion assembly. Sequential recruitment protein coats sorting nexins (SNXs) creates membrane zones at EEs serve as scaffolds for tubulation retrieval cargo proteins, including host cell signaling proteins viral glycoproteins. This study aims investigate whether SNX3-dependent zone contributes CMV Methods: Protein localization...
Background/Objectives: Cytomegalovirus (CMV) infection expands early endosomes (EEs) into tubular extensions that may contribute to the control of virus replication and virion assembly. Sequential recruitment protein coats sorting nexins (SNXs) creates membrane zones at EEs serve as scaffolds for tubulation retrieval cargo proteins, including host cell signaling proteins viral glycoproteins. This study aims investigate whether SNX3-dependent zone contributes CMV Methods: Protein localization...
It has been claimed that MHC class I proteins serve as receptors for murine cytomegalovirus (MCMV) and this interaction is the most important mechanism virus entry in cells. This claim based on observation haplotype contributes to susceptibility (CMV) infection vivo. Results from vitro studies support concept stable expression of correctly folded molecules infection, since individual properties alleles, availability β 2-microglobulin (β 2m) also degree peptide charging heavy chain 2m...
Introduction Cytomegaloviruses (CMVs) extensively reorganize the membrane system of cell and establish a new structure as large nucleus called assembly compartment (AC). Our previous studies on murine CMV (MCMV)-infected fibroblasts indicated that inner part AC contains rearranged early endosomes, recycling endosomal compartments trans-Golgi structures are tubulated, including expansion retention tubular Rab10 elements. An essential process initiates Rab10-associated tubulation is cargo...