A5042448262- Muscle metabolism and nutrition
- Muscle Physiology and Disorders
- Diet and metabolism studies
- Nutrition and Health in Aging
- Sports Performance and Training
- Adipose Tissue and Metabolism
- Exercise and Physiological Responses
- Cardiovascular and exercise physiology
- Metabolism and Genetic Disorders
- Muscle activation and electromyography studies
- Metabolomics and Mass Spectrometry Studies
- Genetics and Physical Performance
- Cystic Fibrosis Research Advances
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Mitochondrial Function and Pathology
- Pharmacological Effects and Assays
- Body Composition Measurement Techniques
- Advanced biosensing and bioanalysis techniques
- Neonatal Respiratory Health Research
- Genetics, Aging, and Longevity in Model Organisms
- Vitamin D Research Studies
- Ion channel regulation and function
- Clinical Nutrition and Gastroenterology
- Children's Physical and Motor Development
University of Nottingham
2016-2025
Royal Derby Hospital
2016-2025
Versus Arthritis
2015-2025
Nottingham Biomedical Research Centre
2017-2025
NIHR Biomedical Research Centre at The Royal Marsden and the ICR
2017-2025
Medical Research Council
2013-2024
National Institute for Health Research
2017-2024
Duke Medical Center
2024
Queen's Medical Centre
2023-2024
Nottingham University Hospitals NHS Trust
2024
The cystic fibrosis transmembrane conductance regulator (CFTR) is associated with expression of a chloride that defective in (CF). Xenopus oocytes injected RNA coding for CFTR contained mutations the first nucleotide binding fold (NBF1) expressed currents response to raising adenosine 3′,5′-monophosphate (cAMP) forskolin and 3-isobutyl-1-methylxanthine (IBMX). mutant CFTRs were less sensitive than wild-type this activating stimulus, reduction sensitivity correlated severity patients carrying...
Maintenance of skeletal muscle mass is contingent upon the dynamic equilibrium (fasted losses-fed gains) in protein turnover. Of all nutrients, single amino acid leucine (Leu) possesses most marked anabolic characteristics acting as a trigger element for initiation synthesis. While mechanisms by which Leu 'sensed' have been subject great scrutiny, branched-chain acid, can be catabolized within muscle, thus posing possibility that metabolites could involved mediating effect(s) Leu. Our...
Resistance exercise training (RET) is widely used to increase muscle mass in athletes and also aged/cachectic populations. However, the time course metabolic molecular control of hypertrophy remain poorly defined. Using newly developed deuterium oxide (D2O)-tracer techniques, we investigated relationship between long-term protein synthesis (MPS) hypertrophic responses RET. A total 10 men (23 ± 1 yr) undertook 6 wk unilateral (1-legged) RET [6 × 8 repetitions, 75% repetition maximum (1-RM)...
Key points Resistance exercise training (RET) is one of the most effective strategies for preventing declines in skeletal muscle mass and strength with age. Hypertrophic responses to RET age are diminished compared younger individuals. In response 6 weeks RET, we found blunted hypertrophic underpinned by chronic deficits long‐term protein synthesis. We show this likely be result multifactorial anabolic hormones translational efficiency capacity. These results provide great insight into...
Quantification of muscle protein synthesis (MPS) remains a cornerstone for understanding the control mass. Traditional [ 13 C]amino acid tracer methodologies necessitate sustained bed rest and intravenous cannulation(s), restricting studies to ∼12 h, thus cannot holistically inform on diurnal MPS. This limits insight into regulation habitual metabolism in health, aging, disease while querying utility techniques predict long-term efficacy anabolic/anticatabolic interventions. We tested D 2 O...
Dysregulated anabolic responses to nutrition/exercise may contribute sarcopenia; however, these characteristics are poorly defined in female populations. We determined the effects of two feeding regimes older women (66 ± 2.5 yr; n = 8/group): bolus whey protein (WP-20 g) or novel low-dose leucine-enriched essential amino acids (EAA) [LEAA; 3 g (40% leucine)]. Using [(13)C6]phenylalanine infusions, we quantified muscle (MPS) and albumin (APS) synthesis at baseline response both (FED) plus...
Impaired anabolic responses to nutrition and exercise contribute loss of skeletal muscle mass with ageing (sarcopenia). Here, we tested protein synthesis (MPS), in the under represented group older women, leucine-enriched essential amino acids (EAA) comparison a large bolus whey (WP).Twenty-four women (65 ± 1 y) received (N = 8/group) 1.5 g EAA supplements (LEAA_1.5), 6 LEAA (LEAA_6) 40 WP. A primed constant I.V infusion 13C6-phenylalanine was used determine MPS at baseline response feeding...
The Vitamin D receptor (VDR) has been positively associated with skeletal muscle mass, function and regeneration. Mechanistic studies have focused on the loss of receptor, in vivo whole-body knockout models demonstrating reduced myofibre size impaired development. To understand mechanistic role upregulation VDR elicits mass/health, we studied impact over-expression (OE) before exploring importance expression upon hypertrophy humans. Wistar rats underwent electrotransfer (IVE) to overexpress...
Despite males typically exhibiting greater muscle strength and fatigability than females, it remains unclear if there are sex-based differences in neuromuscular recruitment strategies e.g. modulation of motor unit firing rate (MU FR) at normalized forces during progressive increases force.The study includes 29 healthy male 31 female participants (18-35 years). Intramuscular electromyography (iEMG) was used to record individual potentials (MUPs) near-fibre MUPs from the vastus lateralis (VL)...
Abstract Cotadutide is a dual glucagon-like peptide 1 and glucagon receptor agonist under development for the treatment of non-alcoholic steatohepatitis type 2 diabetes mellitus (T2DM) chronic kidney disease. Non-alcoholic complex disease with no approved pharmacotherapies, arising from an underlying state systemic metabolic dysfunction in association T2DM obesity. has been shown to improve glycaemic control, body weight, lipids, liver fat, inflammation fibrosis. We conducted two-part,...
Focal adhesion kinase (FAK) is an attachment complex protein associated with the regulation of muscle mass through as-of-yet unclear mechanisms. We tested whether FAK functionally important for hypertrophy, hypothesis that knockdown (FAK-KD) would impede cell growth a trophic stimulus. C 2 12 skeletal cells harboring FAK-targeted or scrambled (SCR) shRNA were created using lentiviral transfection techniques. Both FAK-KD and SCR myotubes incubated 24 h IGF-I (10 ng/ml), additional (±IGF-1)...
We recently reported that the greatest distinguishing feature between eccentric (ECC) and concentric (CON) muscle loading lays in architectural adaptations: ECC favors increases fascicle length (Lf), associated with distal vastus lateralis (VL) hypertrophy, CON pennation angle (PA). Here, we explored interactions structural morphological remodeling, assessed by ultrasound dual x-ray absorptiometry (DXA), long-term protein synthesis (MPS), evaluated deuterium oxide (D2O) tracing technique....
Mechanisms underlying the relationship between systemic inflammation and age-related decline in muscle mass are poorly defined. The purpose of this work was to investigate inflammatory marker CRP elderly identify mechanisms by which mediates its effects on skeletal muscle, in-vitro.Muscle serum level were determined a cohort 118 older women (67±1.7 years). Human cells differentiated into myotubes exposed CRP. size after immunofluorescent staining using troponin. Muscle protein synthesis...