A5100335079- Cell death mechanisms and regulation
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Enzyme Production and Characterization
- Bioactive Compounds and Antitumor Agents
- NF-κB Signaling Pathways
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Autophagy in Disease and Therapy
- Seaweed-derived Bioactive Compounds
- Ubiquitin and proteasome pathways
- Amino Acid Enzymes and Metabolism
- Retinoids in leukemia and cellular processes
- Geometric Analysis and Curvature Flows
- Genomics and Chromatin Dynamics
- Plant Pathogens and Fungal Diseases
- Melanoma and MAPK Pathways
- Agriculture, Soil, Plant Science
- Biochemical effects in animals
- Protein Tyrosine Phosphatases
- Protein Hydrolysis and Bioactive Peptides
- Developmental Biology and Gene Regulation
- Cancer Mechanisms and Therapy
Kyungpook National University
2015-2024
Korea Food Research Institute
2017
Kosin University
2010
Hanyang University
2007
National Institutes of Health
1999-2005
National Heart Lung and Blood Institute
2005
Georgetown University
1999
Soonchunhyang University Hospital
1990
Transcriptional repression by sequence-specific DNA binding factors is mediated the recruitment of a corepressor complex to promoter region. The NK-3 homeodomain protein transcriptional repressor that recruits nuclear kinase, interacting kinase 2 (HIPK2). Here we show HIPK2 component containing Groucho and histone deacetylase complex. Groucho, like HIPK2, acts as for binds HIPK2. Moreover, appears regulate activity Groucho. relieved inhibitor trichostatin A, both directly interact with HDAC1...
Groucho function is essential for Drosophila development, acting as a corepressor specific transcription factors that are downstream targets of various signaling pathways. Here we provide evidence phosphorylated by the DHIPK2 protein kinase. Phosphorylation modulates activity attenuating its protein-protein interaction with DNA-bound factor. During eye modifies activity, and phenotypes generated overexpression differ depending on phosphorylation state. Moreover, analysis nuclear extracts...
The cytoprotective mechanism of l-serine against oxidative stress-mediated neuronal apoptosis was investigated in mouse hippocampal HT22 cells. Treatment with the reactive oxygen species (ROS) inducer 2,3-dimethoxy-1,4-naphthoquinone (DMNQ) increased cytosolic and mitochondrial ROS apoptosis, without necrosis, ROS-mediated accompanied by induction endoplasmic reticulum (ER) apoptotic pathway, involving CHOP/GADD153 upregulation, JNK p38 MAPK activation, caspase-12 caspase-8 subsequent...
<i>NK-4</i> (<i>tinman</i>) encodes an NK-2 class homeodomain transcription factor that is required for development of the <i>Drosophila</i> dorsal mesoderm, including heart. Genetic evidence suggests its important role in mesoderm subdivision, yet properties NK-4 as a transcriptional regulator and mechanism gene by are not completely understood. Here, we describe interaction with p300 coactivator Groucho corepressor. We demonstrate can activate or repress target genes cultured cells,...
A flavonoid antioxidant quercetin promotes dose-dependent activation of the ATM-CHK-p53 pathway, downregulation antiapoptotic survivin, and upregulation proapoptotic NOXA in human T cell acute lymphoblastic leukemia Jurkat clones (J/Neo J/BCL-XL). However, BAG3 MCL-1 occurred J/Neo cells but not J/BCL-XL overexpressing BCL-XL. Additionally, several BCL-XL-sensitive intrinsic mitochondrial apoptotic events including sub-G1 accumulation, TUNEL-positive DNA fragmentation, BAK activation,...
Human lysosomal-associated protein multispanning membrane 5 (LAPTM5) was identified by an ordered differential display-polymerase chain reaction (ODD-PCR) as up-regulated cDNA fragment during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of U937 cells into monocytes/macrophages. After TPA-treatment, the levels LAPTM5 mRNA and increased reached a maximum at 18–36 h. In healthy human tissues, expressed high in hematopoietic low lung fetal liver, not detected other...
Neoagarobiose (NA2) is the repeating disaccharide unit of agarose and possesses various promising biological activities. To identify an efficient exolytic β-agarase required for NA2 production from agarose, GH50A gene agar-degrading Cellvibrio sp. KY-GH-1 was overexpressed as a recombinant His-tagged protein using Escherichia coli expression system. that consists 797 amino acids able to produce predominantly at optimal temperature pH 35 °C 7.5, respectively. The enzyme stable up within range...
In vitro antitumor activity of the CDK7 inhibitor BS-181 against human T-ALL Jurkat cells was determined. Treatment clones (JT/Neo) with caused cytotoxicity and several apoptotic events, including TRAIL/DR4/DR5 upregulation, c-FLIP down-regulation, BID cleavage, BAK activation, ΔΨm loss, caspase-8/9/3 PARP cleavage. However, BCL-2-overexpressing clone (JT/BCL-2) abrogated these responses. catalyzed activating phosphorylation CDK1 (Thr161) CDK2 (Thr160), CDK-directed retinoblastoma attenuated...