A5027334841- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Advanced Chemical Sensor Technologies
- HER2/EGFR in Cancer Research
- Electrochemical sensors and biosensors
- SARS-CoV-2 detection and testing
- Sulfur-Based Synthesis Techniques
- High-pressure geophysics and materials
- Nanoplatforms for cancer theranostics
- Photonic and Optical Devices
- Mechanical and Optical Resonators
- Neurobiology and Insect Physiology Research
- 3D Printing in Biomedical Research
- Peptidase Inhibition and Analysis
- Photochromic and Fluorescence Chemistry
- Advanced Biosensing Techniques and Applications
- Chemical Synthesis and Reactions
- Pulsars and Gravitational Waves Research
ETH Zurich
2020-2025
Bioengineering (Switzerland)
2022-2025
Imperial College London
2019-2025
NIHR Imperial Biomedical Research Centre
2021-2025
Institute for Biomedical Engineering
2025
University College London
2015-2023
Albuquerque Academy
2022-2023
Rutherford Appleton Laboratory
2022-2023
University of Birmingham
2021
Liverpool Hope University
2021
Paper-based lateral flow immunoassays (LFIAs) are one of the most widely used point-of-care (PoC) devices; however, their application in early disease diagnostics is often limited due to insufficient sensitivity for requisite sample sizes and short time frames PoC testing. To address this, we developed a serum-stable, nanoparticle catalyst-labeled LFIA with surpassing that both current commercial published sensitivities paper-based detection p24, earliest conserved biomarkers HIV. We report...
Recent advances in nanomedicine have shown that dramatic improvements nanoparticle therapeutics and diagnostics can be achieved through the use of disease specific targeting ligands.
Enabling oriented installation of non-engineered antibody fragments on nanoparticle surfaces to create next-generation antibody–nanoparticle conjugates.
Driven by complex and interconnected factors, including population growth, climate change, geopolitics, infectious diseases represent one of the greatest healthcare challenges 21st century. Diagnostic technologies are first line defense in fight against disease, providing critical information to inform epidemiological models, track diseases, decide treatment choices, ultimately prevent epidemics. The diagnosis disease at genomic level using nucleic acid biomarkers has proven be most...
Microfluidic paper-based analytical devices (µPADs) are indispensable tools for disease diagnostics. The integration of electronic components into µPADs enables new device functionalities and facilitates the development complex quantitative assays. Unfortunately, current electrode fabrication methods often hinder capillary flow, considerably restricting µPAD design architectures. Here, laser-induced graphenization is presented as an approach to fabricate porous electrodes embedded cellulose...
Herein we report novel protocols for the generation and application of dibromopyridazinediones, an exciting class disulfide bridging reagents.
The role of monoclonal antibodies as vehicles to deliver payloads has evolved a powerful tool in cancer therapy recent years. clinical development therapeutic antibody conjugates with precise holds great promise for targeted interventions. use affinity-peptide mediated functionalization native off-the-shelf offers an effective approach selectively modify IgG drug-antibody ratio (DAR) 2. Here, we report the traceless, peptide-directed attachment two hydroxylamines IgGs followed by...
A novel reagent/strategy enables the controlled assembly of antibody conjugates with a loading two modules without engineering.
The development of low-cost, disposable electrochemical sensors is an essential step in moving traditionally inaccessible quantitative diagnostic assays toward the point need. However, a major remaining limitation current technologies reliance on standardized reference electrode materials. Integrating these electrodes considerably restricts choice substrate and drastically increases fabrication costs. Herein, we demonstrate that adoption two-electrode detection systems can circumvent...
Semiconducting polymer nanoparticles (SPNs) are explored for applications in cancer theranostics because of their high absorption coefficients, photostability, and biocompatibility. However, SPNs susceptible to aggregation protein fouling physiological conditions, which can be detrimental vivo applications. Here, a method achieving colloidally stable low-fouling is described by grafting poly(ethylene glycol) (PEG) onto the backbone fluorescent semiconducting polymer,...
Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated Protein (CRISPR-Cas) systems have evolved several mechanisms to specifically target foreign DNA. These properties made them attractive as biosensors. The primary drawback associated with contemporary CRISPR-Cas biosensors is their weak signaling capacity, which typically compensated for by coupling the nucleic acid amplification. An alternative strategy improve capacity engineer reporter, i.e., design new...
Despite their simplicity, lateral flow immunoassays (LFIAs) remain a crucial weapon in the diagnostic arsenal, particularly at point-of-need. However, methods for analysing LFIAs still rely heavily on sub-optimal human readout and rudimentary end-point analysis. This negatively impacts both testing accuracy times, ultimately lowering throughput. Herein, we present an automated computational imaging method processing multiple real-time parallel. relies detection of signal intensity test line,...
CRISPR-based diagnostics have gained increasing attention as biosensing tools able to address limitations in contemporary molecular diagnostic tests. To maximise the performance of assays, much effort has focused on optimizing chemistry and biology reaction. However, less been paid improving techniques used analyse data. date, decisions typically involve various forms slope-based classification. Such methods are superior traditional based assessing absolute signals, but still limitations....
Immunoreagents, most commonly antibodies, are integral components of lateral flow immunoassays. However, the use antibodies comes with limitations, particularly relating to their reproducible production, and poor thermal chemical stability. Here, we employ phage display develop affibodies, a class nonimmunoglobulin affinity proteins based on small three-helix bundle scaffold, against SARS-CoV-2 Spike protein. Subsequently, demonstrate utility viability affibodies directly replace in In...
CRISPR-based diagnostics have gained increasing attention as biosensing tools able to address limitations in contemporary molecular diagnostic tests. To maximize the performance of assays, much effort has focused on optimizing chemistry and biology reaction. However, less been paid improving techniques used analyze data. date, decisions typically involve various forms slope-based classification. Such methods are superior traditional based assessing absolute signals, but still limitations....
Due to their ability selectively target pathogen-specific nucleic acids, CRISPR-Cas systems are increasingly being employed as diagnostic tools. "One-pot" assays that combine acid amplification and (NAAT–CRISPR-Cas) in a single step have emerged one of the most popular biosensing formats. However, operational simplicity comes at cost, with one-pot typically less sensitive than corresponding two-step NAAT–CRISPR-Cas often failing detect targets low concentrations. It is thought these...
Approximately 1200 nucleotides of sequence data from the promoter and 5'-flanking regions each three pea (Pisum sativum L.) legumin genes (legA, legB legC) are presented. The all were found to be identical including "TATA box", "CAAT box', sequences showing homology SV40 enhancers. legA begins diverge others about 300bp start codon, whereas other two remain for another 550bp. partial exhibit deletions or insertions some short, comparatively well conserved sequences. significance these...
A facile, one-pot procedure for the conversion of aromatic aldehydes to esters, as well thioesters and amides, <italic>via</italic> acyl hydrazide intermediates.
Antibody-targeted nanoparticles have shown exceptional promise as delivery vehicles for anticancer drugs, although manufacturability challenges hampered clinical progress. These include the potential uncontrolled and random antibody conjugation, resulting in masked or inactive paratopes unwanted Fc domain interactions. To circumvent these issues, we show that interchain disulfide of cetuximab F(ab) may be selectively re-bridged with a strained alkyne handle, to permit 'click' coupling...