A5020861603- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Marine and coastal ecosystems
- Oil Spill Detection and Mitigation
- Microbial bioremediation and biosurfactants
- Viral gastroenteritis research and epidemiology
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Environmental DNA in Biodiversity Studies
- Marine Biology and Ecology Research
- Plant Virus Research Studies
- Marine Bivalve and Aquaculture Studies
- Microplastics and Plastic Pollution
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Coastal and Marine Management
- Marine and coastal plant biology
- Respiratory viral infections research
- Isotope Analysis in Ecology
- Marine Ecology and Invasive Species
- Identification and Quantification in Food
- Fluid Dynamics Simulations and Interactions
- Vibrio bacteria research studies
- Petroleum Processing and Analysis
- Fire dynamics and safety research
Bedford Institute of Oceanography
2016-2024
Fisheries and Oceans Canada
2017-2024
University of South Alabama
2009-2023
Government of Canada
2023
Dauphin Island Sea Lab
2009-2016
Montana State University
2006-2012
Planta
2007
Institute of Plant Biology
2007
University of British Columbia
2002-2005
The Yellowstone caldera contains the most numerous and diverse geothermal systems on Earth, yielding an extensive array of unique high-temperature environments that host a variety deeply-rooted understudied Archaea, Bacteria Eukarya. combination extreme temperature chemical conditions encountered in often results considerably less microbial diversity than other terrestrial habitats offers tremendous opportunity for studying structure function indigenous communities establishing linkages...
ABSTRACT Virus enumeration by epifluorescence microscopy (EFM) is routinely done on preserved, refrigerated samples. Concerns about obtaining accurate and reproducible estimates led us to examine procedures for counting viruses EFM. Our results indicate that aldehyde fixation in rapid decreases viral abundance. By 1 h postfixation, the abundance dropped 16.4% ± 5.2% ( n = 6), 4 h, was 20 35% lower. The average loss rates glutaraldehyde- formaldehyde-fixed samples over first 2 were 0.12 0.13...
ABSTRACT Little is known about the replication cycle of archaeal viruses. We have investigated ultrastructural changes Sulfolobus solfataricus P2 associated with infection by t urreted i cosahedral v irus (STIV). A time course a near synchronous STIV was analyzed using both scanning and transmission electron microscopy. Assembly particles, including particles lacking DNA, observed within cells, fully assembled were visible 30 h postinfection (hpi). determined to be lytic virus, causing cell...
ABSTRACT Icosahedral nontailed double-stranded DNA (dsDNA) viruses are present in all three domains of life, leading to speculation about a common viral ancestor that predates the divergence Eukarya , Bacteria and Archaea . This suggestion is supported by shared general architecture this group fold their major capsid protein. However, limited information on diversity replication archaeal viruses, general, has hampered further analysis. Sulfolobus turreted icosahedral virus (STIV), isolated...
Microarray analysis of infection by Sulfolobus turreted icosahedral virus (STIV) revealed insights into the timing and extent transcription, as well differential regulation host genes. Using a microarray containing genes from both virus, cycle STIV was studied. Following solfataricus strain 2-2-12 with STIV, transcription first detected at 8 h postinfection (p.i.), peak 24 p.i. Lysis cells 32 There little temporal control genes, although three open reading frames on noncoding strand were...
Most of the studies microbial processes in response to Deepwater Horizon oil spill focused on deep water plume, and not surface communities. The effects crude application dispersants coastal food web northern Gulf Mexico have been well characterized even though these regions support much fisheries production Gulf. A mesocosm experiment was carried out determine how community off coast Alabama may responded influx dispersants. While addition glucose or alone resulted an increase biomass...
Viruses are the largest reservoir of genetic material on planet, yet little is known about population dynamics any virus within its natural environment. Over a 2-year period, we monitored diversity two archaeal viruses found in hot springs Yellowstone National Park (YNP). Both temporal phylogeny and neutral biodiversity models reveal that these local environments not being maintained by mutation but rather high rates immigration from globally distributed metacommunity. These results indicate...
Marine environments harbour a vast diversity of micro-eukaryotic organisms (protists and other small eukaryotes) that play important roles in structuring marine ecosystems. However, micro-eukaryote is not well understood. Likewise, knowledge limited regarding spatial seasonal distribution, especially over long temporal scales. Given the importance this group for mobilizing energy from lower trophic levels near base food chain to larger organisms, assessing community stability, resilience...
Marine bacterial communities show strong spatial and seasonal patterns, often characterized by changes at high taxonomic levels. The Pelagibacteraceae are common members of communities, with well-documented biogeography the subclade level. To identify patterns within subclades, abundance diversity were analyzed over a two-year period four stations across an estuarine gradient. was most abundant family, averaging 27% community, but varying from 1% to 57% in any one sample. Highest abundances...
ABSTRACT Viruses utilize a diverse array of mechanisms to deliver their genomes into hosts. While great strides have been made in understanding the genome delivery eukaryotic and prokaryotic viruses, little is known about archaeal virus associated particle changes. The Sulfolobus turreted icosahedral (STIV) double-stranded DNA (dsDNA) that contains host-derived membrane sandwiched between proteinaceous capsid shell. Using cryo-electron microscopy (cryo-EM) different biochemical treatments,...
Where there is life, are viruses. The impact of viruses on evolution, global nutrient cycling, and disease has driven research their cellular molecular biology. Knowledge exists for a wide range viruses; however, major exception with archaeal hosts. Archaeal virus-host systems great interest because they have similarities to both eukaryotic bacterial often live in extreme environments. Here we report the first proteomics-based experiments host response viral infection. Sulfolobus Turreted...
The origin and evolutionary relationship of viruses is poorly understood. This makes archaeal virus-host particular interest because the hosts generally root near base phylogenetic trees, while some have clear structural similarities to those that infect prokaryotic eukaryotic cells. Despite advantageous position for use in studies, little known about or how they interact with their hosts, compared bacteria eukaryotes. In addition, many been isolated from extreme environments present a...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 504:13-26 (2014) - DOI: https://doi.org/10.3354/meps10783 Benthic nutrient fluxes and limited denitrification in a sub-tropical groundwater-influenced coastal lagoon Rebecca J. Bernard1,2, Behzad Mortazavi1,2,*, Lei Wang1,3, Alice C. Ortmann1,3, Hugh MacIntyre4, William Burnett5 1The Dauphin Island Sea Lab,...
Heavy crude oil transportation over land is increasing, yet the ecological impacts of spills, particularly diluted bitumen, in freshwater environments remain poorly understood. We simulated spills bitumen 1400 L land-based mesocosms containing water and sediments from a boreal, oligotrophic lake monitored response natural planktonic communities 11 days. Most species phytoplankton (chrysophytes dinoflagellates) zooplankton (copepods cladocerans) were sensitive to oil, exhibiting >70%...
Diluted bitumens are produced by adding lower viscosity diluent to highly viscous bitumen enable it flow through pipelines and thus may behave differently than conventional oils when spilled into coastal seawater. Simulated surface spills using three different diluted products were carried out in May, July November water column hydrocarbons monitored over a 14 day period. Volatile total petroleum varied the depending on season type of diluent. In summer, with synthetic crude or mixture...