A5088343289- Antiplatelet Therapy and Cardiovascular Diseases
- Edible Oils Quality and Analysis
- Advanced Glycation End Products research
- Eicosanoids and Hypertension Pharmacology
- Atrial Fibrillation Management and Outcomes
- Pharmaceutical Practices and Patient Outcomes
- Inflammatory mediators and NSAID effects
- Cholesterol and Lipid Metabolism
- Pharmaceutical studies and practices
- Biochemical effects in animals
- Fatty Acid Research and Health
- Retinal Diseases and Treatments
- Sulfur Compounds in Biology
- Venous Thromboembolism Diagnosis and Management
- Antibiotics Pharmacokinetics and Efficacy
- Antioxidant Activity and Oxidative Stress
- Liver Disease Diagnosis and Treatment
- Medication Adherence and Compliance
- Nitric Oxide and Endothelin Effects
- Free Radicals and Antioxidants
- Cardiac Arrhythmias and Treatments
- Hepatitis B Virus Studies
- Folate and B Vitamins Research
- Liver Disease and Transplantation
- Health Systems, Economic Evaluations, Quality of Life
Universidad de Málaga
2016-2025
Instituto de Investigación Biomédica de Málaga
2015-2025
Andalusian Centre for Nanomedicine and Biotechnology
2024
Faculdade de Medicina do ABC
2016
Instituto de la Grasa
2008
Universidad de Sevilla
2008
Marymount University
2007
Hospital Materno-Infantil
2003
Clinics Hospital of Ribeirão Preto
2002
Hospital Doctor José Molina Orosa
1989
Pharmacology has broadened its scope considerably in recent decades. Initially, it was of interest to chemists, doctors and pharmacists. In years, however, been incorporated into the teaching biologists, molecular biotechnologists, chemical engineers many health professionals, among others. Traditional methods, such as lectures or laboratory work, have superseded by use new pedagogical approaches enable a better conceptualization understanding discipline. this article, we present several...
In the current public health framework, importance of medication as a determinant citizens' has emerged factor warranting special attention. Most studies investigating relationship between and quality life do so from perspective adherence. However, other medication-related factors identified at home visits may be associated with health-related life. Methods design Objective: To describe in patients older than 65 years who use multiple medications (polypharmacy). Design: Cross-sectional...
Virgin olive oil (VOO) contains the polyphenols hydroxytyrosol (HT) and acetate (HT-AC). This study investigated antiplatelet effect of HT HT-AC in healthy rats compared their effects to acetylsalicylic acid (ASA). All compounds were administered orally for 7 days. inhibited platelet aggregation whole blood, with a 50% inhibitory dose (ID50) 48.25 mg/kg per day HT, 16.05 HT-AC, 2.42 ASA. Platelet synthesis thromboxane B2 was by up 30% 37% HT-AC; ID50 this ASA 1.09 day. Vascular prostacyclin...
Abstract We investigated how virgin olive oil (VOO) affected platelet and hypoxic brain damage in rats. Rats were given VOO orally for 30 days at 0.25 or 0.5 mL kg −1 per day (doses A B, respectively). Platelet aggregation, thromboxane B 2 , 6‐keto‐PGF 1α nitrites + nitrates measured, was evaluated a hypoxia–reoxygenation assay with fresh slices. Oxidative stress, prostaglandin E nitric oxide pathway activity lactate dehydrogenase (LDH) also measured. Dose inhibited aggregation by 36% 19%;...
Hydroxytyrosol acetate (HT-AC) is a polyphenol present in virgin olive oil (VOO) at proportion similar to hydroxytyrosol (HT) (160–479 μmol/kg oil). The study was designed measure the vitro platelet antiaggregating activity of HT-AC human whole blood, and compare this effect with that HT acetylsalicylic acid (ASA). experiments were according standard procedure investigate ASA. inhibited aggregation induced by ADP, collagen or arachidonic both blood platelet-rich plasma (PRP). ASA had greater...
We used an animal model of extrahepatic biliary obstruction 7 days' duration to study the production thiobarbituric acid reactive substances (TBARS), total glutathione (TG), reduced (GSH), and oxidized (GSSG), enzymatic activities GSH- peroxidase, GSSG-reductase, GSH-transferase. Four groups six rats each were treated with saline, drug solvent, S-adenosyl-L-methionine (SAM) 5 mg/kg/d, subcutaneously, or SAM 10 subcutaneously. Extrahepatic increased TBARS. had dose-dependent effects...
The aim of this study was to analyze the possible nephroprotective effect 3’,4’-dihydroxyphenylglycol (DHPG), a polyphenolic compound extra virgin olive oil (EVOO), on renal lesions in an experimental model type 1 diabetes. Rats were distributed as follows: healthy normoglycemic rats (NDR), diabetic treated with saline (DR), and DR 0.5 mg/kg/day or DHPG. showed significantly higher serum oxidative nitrosative stress profile than NDR, well reduced prostacyclin production damage (defined...
A series of nitroderivatives has been synthesized from hydroxytyrosol, the natural olive oil phenol, to increase assortment compounds with putative effects against Parkinson's disease. Nitrohydroxytyrosyl esters were obtained nitrohydroxytyrosol using a chemoselective one-step, high-yield, transesterification procedure. The antioxidant activity these new nitrocatechols was evaluated FRAP, ABTS, and ORAC assays compared that free hydroxytyrosol. nitro functional group induced significant in...
The neuroprotective effect of virgin olive oil (VOO) polyphenols has been related to their antioxidant effect. main objective was analyze how tyrosol and hydroxytyrosol contribute the effects VOO in a model hypoxia–reoxygenation rat brain slices. Rats were treated per os (po) (10 or 20 mg/kg/day) with ethyl ether (HTEE), (TEE), 3,4-di-o-methylidene-hydroxytyrosol (MHTEE), used as negative control for effects. Lipid peroxidation inhibited HTEE, TEE, MHTEE (from 5.0 ± 1.5 2.6 1.5, 4.5 4.8...
The aim of the study was to analyze possible neuroprotective effect hydroxytyrosol (HT) in diabetic animals a model hypoxia-reoxygenation. Rats (10 animals/group) were distributed five groups: nondiabetic rats, control rats (DR), and DR treated for 2 months with 1, 5, or 10 mg/kg/day po HT. At end follow-up, an experimental hypoxia-reoxygenation brain slices tested. group showed increased cell death, oxidative nitrosative stress, increase inflammatory mediators. These alterations...
Acetylsalicylic acid (ASA) reduces the incidence of ischemic stroke mainly through its antithrombotic action; however, it also has a direct neuroprotective effect. The present study was designed to evaluate effect ASA on oxidative stress and activity nitric oxide synthase (NOS) in an vitro model hypoxia rat brain slices. Rat slices were perfused with nitrogen (hypoxia) for maximum 120 min, after which we measured lipid peroxidation, glutathione levels, glutathione-related enzyme activities,...
The aim of the study was to test neuroprotective effect hydroxytyrosol (HT) on experimental diabetic retinopathy. Animals were divided in four groups: (1) control nondiabetic rats, (2) streptozotocin-diabetic rats (DR), (3) DR treated with 1 mg/kg/day p.o. HT, and (4) 5 HT. Treatment HT started 7 days before inducing diabetes maintained for 2 months. In group, total area occupied by extracellular matrix increased, retinal cells decreased; both returned near-control values number ganglion...
The aim of this study was to assess the possible neuroprotective effect 3',4'-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model diabetes and whether is modified by presence another EVOO polyphenol, hydroxytyrosol (HT). assessed hypoxia-reoxygenation brain slices quantifying retinal nerve cells. animals were distributed as follows: (1) normoglycemic rats (NDR), (2) diabetic (DR), (3) DR treated with HT (5 mg/kg/day p.o.), (4) DHPG (0.5...