A5004475432- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Circadian rhythm and melatonin
- Neonatal Respiratory Health Research
- Epigenetics and DNA Methylation
- Pregnancy and Medication Impact
- Air Quality and Health Impacts
- Tissue Engineering and Regenerative Medicine
- Bone fractures and treatments
- Connective tissue disorders research
- Reproductive System and Pregnancy
- Autophagy in Disease and Therapy
- Renal and related cancers
- Hydrogen's biological and therapeutic effects
- Noise Effects and Management
- Spaceflight effects on biology
- Sleep and related disorders
- Sexual function and dysfunction studies
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Pain Mechanisms and Treatments
- Hormonal and reproductive studies
- Adolescent and Pediatric Healthcare
- Endometriosis Research and Treatment
- Adipose Tissue and Metabolism
McGill University
2019-2022
Institut National de la Recherche Scientifique
2014-2021
Universidade Federal de Ciências da Saúde de Porto Alegre
2010-2021
Armand Frappier Museum
2016-2020
Université du Québec à Montréal
2018-2020
Triangle
2019
Indianapolis Zoo
2019
Kentucky Science Center
2019
John Wiley & Sons (United States)
2019
Universidade Federal do Rio Grande do Sul
2010
Abstract Melatonin is highly produced in the placenta where it protects against molecular damage and cellular dysfunction arising from hypoxia/re‐oxygenation‐induced oxidative stress as observed primary cultures of syncytiotrophoblast. However, little known about melatonin its receptors human throughout pregnancy their role villous trophoblast development. The purpose this study was to determine melatonin‐synthesizing enzymes, arylalkylamine N‐acetyltransferase (AANAT) hydroxyindole...
Melatonin has been proposed as a possible treatment for the deleterious effects of hypoxia/reoxygenation (H/R), such autophagy, inflammation, and apoptosis. Pathological pregnancies, preeclampsia, are associated with placental H/R, decreased melatonin synthesis well lower levels in placenta maternal plasma. However, exogenous on inflammation autophagy induced by pregnancy complications H/R await investigation. This study aimed to determine whether protects human primary villous trophoblasts...
Introduction: Several experimental and epidemiological studies have demonstrated the neurological adverse effects caused by exposure to air pollution, specifically in relation pollutant particulate matter (PM). The objective of this study was investigate direct effect PM increased concentrations different brain regions, as well mechanisms involving its neurotoxicity, evaluating oxidative stress parameters vitro.Methods: Olfactory bulb, cerebral cortex, striatum, hippocampus cerebellum rats...
Abstract The syncytiotrophoblast is a multinucleated layer that plays critical role in regulating functions of the human placenta during pregnancy. Maintaining relies on ongoing fusion mononuclear cytotrophoblasts throughout pregnancy, and errors this process are associated with complications such as preeclampsia. While biochemical factors known to drive fusion, disease-specific extracellular biophysical cues remains undefined. Since substrate mechanics play crucial several diseases,...
Melatonin has protective roles in normal cells and cytotoxic actions cancer cells, with effects involving autophagy nuclear factor (erythroid-derived 2)-like 2 (Nrf2) transcription pathways. Hypoxia/reoxygenation (H/R) induces oxidative damage apoptosis. These consequences activate autophagy, which degrades damaged cellular content, as well activates Nrf2 the factor, thereby expression of genes. The current study shows melatonin to differentially modulate pathways tumor placental exposed...
The aims of this study were to evaluate whether air pollution during pre-natal and post-natal phases change habituation short-term discriminative memories if oxidants are involved in process. As secondary objectives, it was the filtered nonfiltered environment could protect cortex rats against oxidative stress as well modify behavior these animals. Wistar, male divided into four groups (n = 12/group): pre exposure until adulthood (FA); period (NFA-FA); (21st day) (PND21); PND21 (FA-NFA);...
This protocol describes how villous cytotrophoblast cells are isolated from placentas at term by successive enzymatic digestions, followed density centrifugation, media gradient isolation and immunomagnetic purification. As observed in vivo, mononucleated primary culture differentiate into multinucleated syncytiotrophoblast after 72 hr. Compared to normoxia (8% O2), that undergo hypoxia/reoxygenation (0.5% / 8% O2) increased oxidative stress intrinsic apoptosis, similar vivo pregnancy...
The placental syncytiotrophoblast is a multinucleated layer that regulates transport between the mother and fetus. Fusion of trophoblasts essential to form this layer, but process can be disrupted in pregnancy-related disorders such as preeclampsia. Disease progression also associated with changes extracellular matrix (ECM), whether disease-specific ECM compositions play any causal role establishing disease phenotypes remains unknown. Here, we develop decellularization-based platform isolate...
The placental syncytiotrophoblast is a giant multinucleated cell that forms tree-like structure and regulates transport between mother baby during development. It maintained throughout pregnancy by continuous fusion of trophoblast cells, disruptions in are associated with considerable adverse health effects including diseases such as preeclampsia. Developing predictive control over culture models hence critical importance drug discovery studies, but this can currently be only partially...
To evaluate the effects of testosterone (T) on maintenance corpus cavernosum (CC) structure and apoptosis.Animals were divided into three groups: sham operation group (n = 8) underwent operation; Orchiectomized (Orchiec)+ oily vehicle bilateral orchiectomy received a single dose by intramuscular injection (i.m.) 30 days after orchiectomy; Orchiec + T undecanoate 100 mg/kg i.m. surgery. Animals euthanized 60 beginning experiment with an anesthetic overdose ketamine xylazine. Blood samples...
This protocol describes how villous cytotrophoblast cells are isolated from placentas at term by successive enzymatic digestions, followed density centrifugation, media gradient isolation and immunomagnetic purification. As observed in vivo, mononucleated primary culture differentiate into multinucleated syncytiotrophoblast after 72 hr. Compared to normoxia (8% O2), that undergo hypoxia/reoxygenation (0.5% / 8% O2) increased oxidative stress intrinsic apoptosis, similar vivo pregnancy...
Highlights H/R induces autophagy and Nrf2 in tumoral primary trophoblast cells. Melatonin inhibits under H/R, inducing BeWo cell death. increases conditions, promoting villous cells survival. Abstract Hypoxia/reoxygenation (H/R) oxidative damage apoptosis. These consequences activate autophagy, which degrades damaged cellular content, as well the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) transcription factor, thereby expression of protective genes. has roles normal cytotoxic actions...