A5083640058- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Selenium in Biological Systems
- RNA regulation and disease
- Trace Elements in Health
- Viral gastroenteritis research and epidemiology
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Fungal and yeast genetics research
- Nuclear Structure and Function
- Glycosylation and Glycoproteins Research
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- Sulfur Compounds in Biology
- Pancreatitis Pathology and Treatment
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
- Heat shock proteins research
- Cancer-related gene regulation
- Mercury impact and mitigation studies
- Redox biology and oxidative stress
Institut de Biologie Moléculaire et Cellulaire
2002-2024
Estonian University of Life Sciences
2024
Architecture et Réactivité de l'arN
2007-2024
Université de Strasbourg
2011-2024
Centre National de la Recherche Scientifique
1993-2018
Russian Academy of Sciences
2009
University of Edinburgh
1998-2005
Institut de Biologie Moléculaire des Plantes
1993-2005
Wellcome Centre for Cell Biology
2000-2005
Sapienza University of Rome
2002
Christine Allmang, Elisabeth Petfalski, Alexandre Podtelejnikov, Matthias Mann, David Tollervey, and Philip Mitchell Institute of Cell Molecular Biology, University Edinburgh, Edinburgh EH9 3JR UK; CEBI Odense University, DK-5230 M, Denmark
Very few of the enzymes required for eukaryotic precursor ribosomal RNA (pre-rRNA) processing have been identified. Ribonuclease (RNase) MRP was characterized as a nuclease that cleaves mitochondrial replication primers, but it is predominantly nucleolar. Previous genetic evidence revealed this ribonucleoprotein required, directly or indirectly, cleavage yeast pre-rRNA in vivo at site A 3 . Here, an vitro system accurately reproduces described. Biochemical purification and use extracts...
The yeast exosome is a complex of 3′→5′ exonucleases involved in RNA processing and degradation. All 11 known components the are required during 3′ end 5.8S rRNA. Here we report that depletion each individual inhibits early pre-rRNA cleavages at sites A0, A1, A2 A3, reducing levels 32S, 20S, 27SA2 27SA3 pre-rRNAs. 27SB pre-rRNAs were also reduced. Consequently, both 18S 25S rRNAs depleted. Since none these steps involves exonuclease activities, requirement for probably indirect. Correct...
RNA-binding proteins of the L7Ae family are at heart many essential ribonucleoproteins (RNPs), including box C/D and H/ACA small nucleolar RNPs, U4 nuclear RNP, telomerase, messenger RNPs coding for selenoproteins. In this study, we show that Nufip its yeast homologue Rsa1 key components machinery assembles these RNPs. We observed bind several tether them to other core in immature particles. Surprisingly, also link assembling with AAA + adenosine triphosphatases hRvb1 hRvb2 Hsp90 chaperone...
Almost all small eukaryotic RNAs are processed from transiently stabilized 3-extended forms.A key question is how and why such intermediates they can then be to the mature RNA.Here we report that yeast U3 also a precursor.The major forms of (U3-3I -II) lack cap trimethylation present in not associated with nucleolar RNP (snoRNP) proteins bind U3, i.e., Nop1p, Nop56p, Nop58p.Depletion Nop58p leads loss but increases level U3-3I -II, indicating requirement for snoRNP final maturation.Pre-U3...
The yeast exosome is a complex of 3' --> 5' exoribonucleases. Sequence analysis identified putative human homologues for components, although several were found only as expressed sequence tags. Here we report the cloning full-length cDNAs, which encode Rrp40p, Rrp41p, and Rrp46p components exosome. Recombinant proteins used to raise rabbit antisera. In Western blotting experiments, these decorated HeLa cell predicted sizes. All three enriched in cells nucleus nucleolus, but also clearly...
The pap1-5 mutation in poly(A) polymerase causes rapid depletion of mRNAs at restrictive temperatures.Residual are polyadenylated, indicating that Pap1-5p retains least partial activity.In strains lacking Rrp6p, a nucleus-specific component the exosome complex 3-5 exonucleases, accumulation ؉ mRNA was largely restored and growth improved.The catalytically inactive mutant Rrp6-1p did not increase strain conferred much less stabilization than rrp6⌬.This may indicate major function Rrp6p is RNA...
Mammalian mRNAs are generated by complex and coordinated biogenesis pathways acquire 5′-end m7G caps that play fundamental roles in processing translation. Here we show several selenoprotein not recognized efficiently translation initiation factor eIF4E because they bear a hypermethylated cap. This cap modification is acquired via maturation pathway similar to of the small nucle(ol)ar RNAs (sn- snoRNAs). Our findings also establish trimethylguanosine synthase 1 (Tgs1) interacts with for...
Depletion of any the essential Lsm proteins, Lsm2–5p or Lsm8p, delayed pre-rRNA processing and led to accumulation many aberrant intermediates, indicating that an complex is required maintain normally strict order events. In addition, high levels degradation products derived from both precursors mature rRNAs accumulated in Lsm-depleted strains. proteins reduced apparent processivity 5′ 3′ exonuclease activities involved 5.8S rRNA processing, intermediates were consistent with inefficient...
Depletion of any the five essential proteins Lsm2p to Lsm5p and Lsm8p leads strong accumulation all tested unspliced pre-tRNA species, as well 5 3 unprocessed species.Aberrant 3-extended pre-tRNAs were detected, presumably due stabilization transcripts that fail undergo correct transcription termination, truncated tRNA fragments was also observed.Tandem affinity purification-tagged Lsm3p associated with primary and, less efficiently, other intermediates but not mature tRNAs.Association...
Selenoprotein synthesis in eukaryotes requires the selenocysteine insertion sequence (SECIS) RNA, a hairpin 3' untranslated region of selenoprotein mRNAs. The SECIS RNA is recognized by SECIS-binding protein 2 (SBP2), which key player this specialized translation machinery. objective work was to obtain structural insight into SBP2-SECIS complex. Multiple alignment revealed that SBP2 and U4 snRNA-binding 15.5 kD/Snu13p share same binding domain L7A/L30 family, also found box H/ACA snoRNP...
Selenoproteins contain the amino acid selenocysteine which is encoded by a UGA Sec codon. Recoding requires complex mechanism, comprising cis-acting SECIS RNA hairpin in 3′UTR of selenoprotein mRNAs, and trans-acting factors. Among these, Binding Protein 2 (SBP2) central to mechanism. SBP2 has been so far functionally characterized only rats humans. In this work, we report characterization Drosophila melanogaster (dSBP2). Despite its shorter length, it retained same synthesis-promoting...
Escherichia coli ribosomal protein S8 was previously shown to bind a 16S rRNA fragment (nucleotides 584–756) with the same affinity as complete rRNA, and shield an irregular helical region (region C) [Mougel, M., Eyermann, F., Westhof, E., Romby, P., Expert‐Bezançon, Ebel, J. Ehresmann, B. & C. (1987). Mol. Biol. 198 , 91–107]. Region C postulated display characteristic features: three bulged adenines (A595, A640 A642), non‐canonical U598–U641 pair surrounded by two G · pairs. In order...
By binding to SECIS elements located in the 3′-UTR of selenoprotein mRNAs, protein SBP2 plays a key role assembly selenocysteine incorporation machinery. contains an L7Ae/L30 RNA-binding domain similar that 15.5K/Snu13p, which binds K-turn motifs with 3-nt bulge loop closed by tandem G.A and A.G pairs. Here, SELEX experiments, we demonstrate capacity bind such protruding U residue. However, show conversion into internal reinforces affinity greater extent RNP stability. Opposite variations...
Selenoprotein synthesis requires the co-translational recoding of a UGASec codon. This process involves an RNA structural element, called Selenocysteine Insertion Sequence (SECIS) and SECIS binding protein 2 (SBP2). Several selenoprotein mRNAs undergo unusual cap hypermethylation by trimethylguanosine synthase 1 (Tgs1), which is recruited ubiquitous Survival MotoNeurons (SMN) protein. SMN, involved in spinal muscular atrophy, part chaperone complex that collaborates with methylosome for RNP...
In homeostasis, which plays an important role in the proper functioning and maintenance of internal body, kidneys play a key being responsible for homeostasis glucose. Among glucose transporters, sodium-dependent co-transporters (SGLTs) have major kidney's ability to reabsorb Although localization these transporters has been extensively studied mammals, there are still gaps knowledge SGLTs birds different age groups. The aim this study was immunolocalize hen chickens ages SGLT1 SGLT2,...
Shigella spp. are the causative agents of shigellosis, which remains a leading cause death in children under age 5. Symptoms shigellosis include bloody diarrhea, associated to colon hemorrhage; more severe cases , bacteremia is induced. These clinical features indicate that exposed and survive exposure plasma, locally systemically, although this has not yet been studied at molecular level. In report, we confirmed guinea pig model both S. flexneri 5a sonnei induced local hemorrhages...