A5079635130- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Animal Virus Infections Studies
- Cancer-related gene regulation
- Plant Molecular Biology Research
- RNA regulation and disease
- Electrochemical Analysis and Applications
- Aluminum toxicity and tolerance in plants and animals
- CRISPR and Genetic Engineering
- Analytical Chemistry and Chromatography
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Telomeres, Telomerase, and Senescence
- RNA Interference and Gene Delivery
- Free Radicals and Antioxidants
- DNA Repair Mechanisms
- Protein Degradation and Inhibitors
HUN-REN Szegedi Biológiai Kutatóközpont
2006-2022
Université Toulouse III - Paul Sabatier
2011-2022
Centre National de la Recherche Scientifique
2007-2022
Hungarian Academy of Sciences
2007-2019
Agricultural Institute
2019
Centre for Agricultural Research
2019
Université de Toulouse
2011-2018
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2015
Lithuanian Ornithological Society
2014
Laboratoire de Biologie Moléculaire des Eucaryotes
1997-2013
Eukaryotic cells contain a large number of small nucleolar RNAs (snoRNAs). A major family snoRNAs features consensus ACA motif positioned 3 nucleotides from the 3' end RNA. In this study we have characterized nine novel human (U64-U72). Structural probing U64 RNA followed by systematic computer modeling all known box revealed that class is defined phylogenetically conserved secondary structure. The fold into two hairpin structures connected single-stranded hinge region and short tail....
Telomerase is a ribonucleoprotein reverse transcriptase that uses its RNA component as template for synthesis of telomeric DNA repeats at the ends linear eukaryotic chromosomes. Here, fluorescence in situ hybridization demonstrates HeLa cancer cells, human telomerase (hTR) accumulates nucleoplasmic Cajal bodies (CBs). Localization transiently expressed hTR to CBs supported by short sequence motif (411-UGAG-414) located 3′-terminal box H/ACA RNA-like domain and structurally functionally...
RNA-binding proteins of the L7Ae family are at heart many essential ribonucleoproteins (RNPs), including box C/D and H/ACA small nucleolar RNPs, U4 nuclear RNP, telomerase, messenger RNPs coding for selenoproteins. In this study, we show that Nufip its yeast homologue Rsa1 key components machinery assembles these RNPs. We observed bind several tether them to other core in immature particles. Surprisingly, also link assembling with AAA + adenosine triphosphatases hRvb1 hRvb2 Hsp90 chaperone...
Pseudouridine, the most abundant modified nucleoside in RNA, is synthesized by posttranscriptional isomerization of uridines. In eukaryotic RNAs, site-specific synthesis pseudouridines directed primarily box H/ACA guide RNAs. this study, we have identified 61 novel putative pseudouridylation RNAs construction and characterization a cDNA library human The majority new are predicted to direct pseudouridine rRNAs spliceosomal small nuclear We can attribute RNA-directed modification 79 97 sites...
Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in synthesis, telomerase has not yet been detected at telomeres. The RNA component of human (hTR) resides the nucleoplasmic Cajal bodies (CBs) interphase cancer cells. Here, situ hybridization demonstrates HeLa and Hep2 S phase cells, besides accumulating CBs, hTR specifically concentrates few telomeres also...
The carboxy-terminal domain (CTD) of the large subunit RNA polymerase II (Pol II) comprises multiple heptapeptide repeats consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation Ser2, Ser5, and Ser7 during transcription cycle mediates sequential recruitment transcription/RNA processing factors. Phosphorylation is required for gene type-specific Integrator complex to Pol II-transcribed small nuclear (sn)RNA genes. Here, we show that II-associated protein 2 (RPAP2)...
Site-specific 2′-O-ribose methylation of mammalian rRNAs and RNA polymerase II-synthesized spliceosomal small nuclear RNAs (snRNAs) is mediated by nucleolar Cajal body (CB)-specific box C/D ribonucleoprotein particles (RNPs) in the nucleolus nucleoplasmic CBs, respectively. Here, we demonstrate that 2′-O-methylation C34 wobble cytidine human elongator tRNA Met (CAT) achieved collaboration a CB-specific RNP carrying SNORD97 SCARNA97 guide RNAs. Methylation prevents site-specific cleavage...
Many small nucleolar RNAs (snoRNAs) in vertebrates are encoded within introns of protein genes. We have reported previously that two isoforms human U17 snoRNA the cell-cycle regulatory gene, RCC1. now investigated mechanism processing and another intron-encoded snoRNA, U19. Experiments which intronic RNA substrates was tested HeLa cell extracts suggest exonucleases rather than endonucleases involved excision U19 RNAs: (1) Cutoff products would be expected from endonucleolytic cleavages were...
The nucleolus has long been known as a functionally highly specialized subnuclear compartment where synthesis, posttranscriptional modification, and processing of cytoplasmic rRNAs take place. In this study, we demonstrate that the contains all thetrans-acting factors are responsible for accurate efficient synthesis eight 2′-O-methylated nucleotides three pseudouridine residues carried by mammalian U6 spliceosomal small nuclear RNA. Factors mediating formation in U3 nucleolar RNA also...