A5106417644- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Crystallography and molecular interactions
- Porphyrin and Phthalocyanine Chemistry
- RNA Research and Splicing
- RNA modifications and cancer
- Metal complexes synthesis and properties
- Magnetism in coordination complexes
- RNA and protein synthesis mechanisms
- Organometallic Complex Synthesis and Catalysis
- Autophagy in Disease and Therapy
- Genomics and Chromatin Dynamics
- Metal-Catalyzed Oxygenation Mechanisms
- DNA Repair Mechanisms
- Ferrocene Chemistry and Applications
- Mitochondrial Function and Pathology
- Surface Chemistry and Catalysis
- Amyotrophic Lateral Sclerosis Research
- Synthetic Organic Chemistry Methods
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Epigenetics and DNA Methylation
- Molecular Junctions and Nanostructures
- Telomeres, Telomerase, and Senescence
- Ubiquitin and proteasome pathways
- Asymmetric Hydrogenation and Catalysis
Imperial College Healthcare NHS Trust
2024
Charing Cross Hospital
2024
Stella Therapeutics (United States)
2020-2021
Columbia University
2007-2020
Université Bourgogne Franche-Comté
2015
Maison des Sciences de l’Homme de Dijon
2015
Centre National de la Recherche Scientifique
1994-2014
Centre National pour la Recherche Scientifique et Technique (CNRST)
2014
Université Toulouse III - Paul Sabatier
2014
New York Proton Center
2014
Gene transcription in the cell nucleus is a complex and highly regulated process. Transcription eukaryotes requires three distinct RNA polymerases, each of which employs its own mechanisms for initiation, elongation, termination. Termination vary considerably, ranging from relatively simple to exceptionally complex. In this review, we describe present state knowledge on how polymerases terminates are conserved, or vary, yeast human.
Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in synthesis, telomerase has not yet been detected at telomeres. The RNA component of human (hTR) resides the nucleoplasmic Cajal bodies (CBs) interphase cancer cells. Here, situ hybridization demonstrates HeLa and Hep2 S phase cells, besides accumulating CBs, hTR specifically concentrates few telomeres also...
Koichi Ogami1,4, Patricia Richard1, Yaqiong Chen1, Mainul Hoque2, Wencheng Li2, James J. Moresco3, John R. Yates III3, Bin Tian2 and L. Manley1 1Department of Biological Sciences, Columbia University, New York, York 10027, USA; 2Department Microbiology, Biochemistry, Molecular Genetics, Rutgers Jersey Medical School, Newark, 07103, 3Department Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037, USA Corresponding author: jlm2{at}columbia.edu ↵4 Present address:...
XRN2 is a 5'-3' exoribonuclease implicated in transcription termination. Here we demonstrate an unexpected role for the DNA damage response involving resolution of R-loop structures and prevention double-strand breaks (DSBs). We show that undergoes damage-inducible nuclear re-localization, co-localizing with 53BP1 R loops, R-loop-dependent process. loss leads to increased genomic instability, replication stress, DSBs hypersensitivity cells various damaging agents. arise occur at...
Senataxin (SETX) is an RNA/DNA helicase implicated in transcription termination and the DNA damage response mutated two distinct neurological disorders: AOA2 (ataxia oculomotor apraxia 2) ALS4 (amyotrophic lateral sclerosis 4). Here we provide evidence that Rrp45, a subunit of exosome, associates with SETX manner dependent on sumoylation. We show interaction sumoylation are disrupted by mutations associated but not ALS4. Furthermore, Rrp45 colocalizes foci upon induction...
New (C^N) cyclometallated Au(<sc>iii</sc>) complexes with cytotoxic properties shown to be potent inhibitors of the zinc finger protein PARP-1.
We studied three patients with mutations in the senataxin gene (SETX). One had juvenile onset of ALS. The second case resembled hereditary motor neuropathy. third patient an overlap syndrome ataxia-tremor and neuron disease, phenotypes previously associated SETX mutations. Our were all apparently sporadic, no other affected relative. Two relatives no. 2 carried c.4660T > G transversion but did not manifest abnormal eye movements, ataxia, or tremor suggesting that genetic environmental...
SETX (senataxin) is an RNA/DNA helicase that has been implicated in transcriptional regulation and the DNA damage response through resolution of R-loop structures. Mutations result either two distinct neurodegenerative disorders. dominant mutations a juvenile form amyotrophic lateral sclerosis (ALS) called ALS4, whereas recessive are responsible for ataxia with oculomotor apraxia type 2 (AOA2). How same protein can lead to different phenotypes still unclear. To elucidate AOA2 disease...
Processing from pre-mRNA introns is a widespread mechanism to generate human box C/D and H/ACA snoRNAs. Recent studies revealed that an optimal position relative the 3′ splice site important for efficient processing of most snoRNAs assembly snoRNPs stimulated by splicing factors likely bound branch point region. Here we have investigated another major class intron-encoded RNAs, Analysis 80 RNA genes RNAs possess no preferential localization close or 5′ site. In vivo experiments confirmed...
Post-transcriptional pseudouridylation and 2′-O-methylation of splicesomal small nuclear ribonucleic acids (snRNAs) is mediated by box H/ACA C/D Cajal body (CB)-specific ribonucleoproteins (scaRNPs), respectively. The WD-repeat protein 79 (WDR79) has been proposed to interact with both classes modification scaRNPs target them into the CB. scaRNAs carry common CAB motif (consensus, ugAG) that required for WDR79 binding CB-specific accumulation. Thus far, no cis-acting CB-localization element...
Functions of Kub5-Hera (In Greek Mythology Hera controlled Artemis) (K-H), the human homolog yeast transcription termination factor Rtt103, remain undefined. Here, we show that K-H has functions in both and DNA double-strand break (DSB) repair. forms distinct protein complexes with factors repair DSBs (e.g. Ku70, Ku86, terminate RNA polymerase II). loss resulted increased basal R-loop levels, DSBs, activated DNA-damage responses enhanced genomic instability. Significantly lowered Artemis...
A GGGGCC repeat expansion in the C9ORF72 (C9) gene is most common known cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. Several mechanisms have been proposed to account for its toxicity, including possibility that reduced C9 protein levels contribute disease. To investigate this possibility, we examined effects several cell systems. We first showed knockdown (KD) U87 glioblastoma cells results striking morphological changes, vacuolization alterations size....
Growing evidence suggests that human gut bacteria, which comprise the microbiome, are linked to several neurodegenerative disorders. An imbalance in bacterial population of Parkinson’s disease (PD) and Alzheimer’s (AD) patients has been detected studies. This dysbiosis very likely decreases or increases microbiome-derived molecules protective detrimental, respectively, body those changes communicated brain through so-called ‘gut-brain-axis’. The molecule queuine is a hypermodified nucleobase...
Cell-surface proteins are central for the interaction of cells with their surroundings and also associated numerous diseases. These molecules exposed to mechanical forces, but exact relation between force functions pathologies cell-surface is unclear. An important protein CD4, primary receptor HIV-1. Here we show that activates conformational chemical changes on CD4 may be during viral attachment. We have used single-molecule spectroscopy analysis HIV-1 infectivity demonstrate extension...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetalloprophyrins containing .sigma.-bonded nitrogen axial ligands. 2. Synthesis and characterization of iron(III) tetrazolato triazolato porphyrin complexes. Molecular structure (5-methyltetrazolato)(2,3,7,8,12,13,17,18-octaethylporphinato)iron(III)R. Guilard, I. Perrot, A. Tabard, P. Richard, C. Lecomte, Y. H. Liu, Karl M. KadishCite this: Inorg. Chem. 1991, 30, 1, 27–37Publication Date (Print):January 1991Publication History Published online1...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTin(II) porphyrins: synthesis and spectroscopic properties of a series divalent tin porphyrins. X-ray crystal structure (2,3,7,8,12,13,17,18-octaethylprophinato)tin(II)J. M. Barbe, C. Ratti, P. Richard, Lecomte, R. Gerardin, GuilardCite this: Inorg. Chem. 1990, 29, 20, 4126–4130Publication Date (Print):October 1, 1990Publication History Published online1 May 2002Published inissue 1 October...
Transcription-related proteins are frequently identified as targets of sumoylation, including multiple subunits the RNA polymerase II (RNAPII) general transcription factors (GTFs). However, it is not known how sumoylation affects GTFs or whether they sumoylated when assemble at promoters to facilitate RNAPII recruitment and initiation. To explore can regulate genome-wide, we performed SUMO ChIP-seq in yeast found, agreement with others, that most chromatin-associated detected genes encoding...
The reaction of [RuCl2(cod)(bpzm)] (1; cod = 1,5-cyclooctadiene, bpzm bis(pyrazol-1-yl)methane) with MeMgCl gives [RuMeCl(cod)(bpzm)] (2). This complex reacts AgCF3SO3, affording [RuMe(CF3SO3)(cod)(bpzm)] (3) and AgCl. [RuTpMe(cod)] (4) [RuTp*Me(cod)] (5) were prepared from the 3 KTp (Tp hydridotris(pyrazolyl)borate) KTp* (Tp* hydridotris(3,5-dimethylpyrazolyl)borate), respectively. Compound 5 was characterized by X-ray diffraction. molecule shows unusual coordination...