A5013431924- CRISPR and Genetic Engineering
- Cancer Research and Treatments
- Occupational and environmental lung diseases
- Oral and Maxillofacial Pathology
- Insect Resistance and Genetics
- Protein Degradation and Inhibitors
- Microtubule and mitosis dynamics
- Hedgehog Signaling Pathway Studies
- Acute Myeloid Leukemia Research
- Advanced biosensing and bioanalysis techniques
- Reproductive Biology and Fertility
- Agriculture, Water, and Health
- Multiple Myeloma Research and Treatments
- Medical Imaging and Pathology Studies
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Wireless Body Area Networks
- Single-cell and spatial transcriptomics
- Manufacturing Process and Optimization
- Cancer Mechanisms and Therapy
- Agricultural Practices and Plant Genetics
- Plant Pathogens and Fungal Diseases
- Molecular Communication and Nanonetworks
- Retinal Development and Disorders
- Enzyme function and inhibition
Emory University
2022-2024
Biomedical Research Foundation
2024
Aichi Medical University
2018-2023
American International University-Bangladesh
2017-2021
Vellore Institute of Technology University
2021
Bangladesh Agricultural Research Institute
2018-2019
Shaheed Suhrawardy Medical College
2018
University of Chittagong
2016-2017
The purpose of this article is to provide information on the history, accomplishments, and future direction Bt brinjal (eggplant) program in Bangladesh, formerly under Agricultural Biotechnology Support Project II, now South Asia Eggplant Improvement Partnership (SAEIP). India-based Maharashtra Hybrid Seed Company (Mahyco) developed an eggplant expressing Cry1Ac (EE-1) for control fruit shoot borer (EFSB). In a partnership among Mahyco, USAID, Sathguru Management Consultants Cornell...
Dysregulation of Hedgehog (Hh) signaling pathway has been documented in mammary gland development and breast cancer (BC) progression. Despite the remarkable progress therapeutic interventions, BC related mortality Bangladesh increased last decade. Triple negative (TNBC) still presents a critical challenge. Thus effective targeted therapy is urgently needed. In this study, we report clinicopathological characteristics prognosis patients from Bangladesh. Routine immunohistochemical analysis...
Abstract Serum from one hundred and ten breast cancer patients thirty healthy female volunteers, were prospectively collected evaluated for serum levels of Shh IL-6 using human specific enzyme-linked immunoassays. All regularly monitored event free survival (EFS) overall (OS). Overall outcome analysis was based on levels. In with progressive metastatic BC, both concentrations elevated in 44% (29 65) 63% (41 patients, respectively, at a statistically significant level [Shh (p = 0.0001)...
We are living in the fourth industrial revolution. Our life is becoming more comfortable and smarter with help of rapid upgrade technology. Internet things (IoT) playing a massive role this. One major sides IoT smart home. As we era never-ending growth internet its application, home system or automation highly increasing to provide comfort improving quality life. In this paper, present an based low-cost system. This on web portal which controlled by ESP32 Wi-Fi module. Also, custom-made...
Abstract 5′ adenosine monophosphate–activated protein kinase–related kinase 5 (ARK5) is involved in mitochondrial ATP production and associated with poor prognosis of multiple myeloma (MM). However, the molecular mechanisms ARK5 MM remain largely unknown. This study examined pathogenic role mitochondria by using genetically modified isogenic cell clones or without human lines, KMS-11 Sachi, which overexpress . The biallelic knockout (ARK5-KO) inhibited proliferation, colony formation,...
Highlights•Tandem paired nicking promotes targeted knockin with substantial efficiency•Tandem scarcely creates indels at the edited genomic loci•DNA recombination elicited by tandem is analogous to Holliday's model•Targeted shows no reciprocal interference p53SummaryTargeted mediated double-stranded DNA cleavage accompanied unwanted insertions and deletions (indels) on-target off-target sites. A nick-mediated approach generates but exhibits reduced efficiency of knockin. Here, we demonstrate...
Eggplant or brinjal (Solanum melongena) is a popular vegetable grown throughout Asia where it attacked by fruit and shoot borer (BFSB) (Leucinodes orbonalis). Yield losses in Bangladesh have been reported up to 86% farmers rely primarily on frequent insecticide applications reduce injury. has developed released four varieties producing Cry1Ac (Bt brinjal) the first country do so. We report replicated field trials comparing Bt their non-Bt isolines, with without standard spray regimes....
Abstract Ameloblastoma is a rare odontogenic benign tumor accounting for less than 1% of head and neck tumors. Advanced next generation sequencing (NGS) analyses identified high frequency BRAF V600E SMO L412F mutations in ameloblastoma. Despite the existence whole genomic sequence information from patients with ameloblastoma, entire molecular signature characteristics ameloblastoma cells are still obscure. In this study, we sought to uncover basis determine cellular phenotype mutations. Our...
Abstract Loss of heterozygosity or mutation the family with sequence similarity 46, member C ( FAM46C ) gene on chromosome band 1p12 is associated shorter overall survival patients multiple myeloma (MM). In this study, using human MM cell lines (KMS‐11, OCI‐My5, and ANBL‐6), we generated −/− clones examined effect disruption cellular signaling. Cell proliferation assays showed increased clonogenicity KMS‐11 cells compared to WT cells. Xenograft experiments significantly mice harboring...
Malignant pleural mesothelioma ( MPM ), a highly refractory tumor, is currently incurable due to the lack of an early diagnosis method and medication, both which are urgently needed improve survival and/or quality life patients. NF 2 tumor suppressor gene frequently mutated in . Using CRISPR /Cas9 system, we generated 2‐ knockout human mesothelial cell line, MeT‐5A KO ). In clones, growth, clonogenic activity, migration invasion activity significantly increased compared with those WT clones....
Abstract Malignant pleural mesothelioma (MPM) is an aggressive malignancy of the pleura that currently incurable due to lack effective early diagnostic method and specific medication. The CDKN2A ( p16 ) NF2 genes are both frequently mutated in MPM. To understand how these mutations contribute MPM tumor growth, we generated NF2/p16 double-knockout (DKO) cell clones using human MeT-5A HOMC-B1 mesothelial lines. Cell growth migration activities were significantly increased DKO compared with...
Abstract Internal tandem duplication (ITD) of FMS-like tyrosine kinase 3 ( FLT3 ) confers poor prognosis and is found in approximately 25% cases acute myeloid leukemia (AML). Although inhibitors have shown clinical benefit patients with AML harboring -ITD, the therapeutic effect limited. Here, to explore alternative therapeutics, we established a cellular model monoallelic ITD/WT cells using CRISPR-Cas9 system human cell line, K562. cDNA microarray analysis revealed elevated CD52 expression...
[Figure: see text] Multiple myeloma (MM) remains an intractable hematological malignancy, despite recent advances in anti-MM drugs. Here, we show that role of PDZ binding kinase (PBK) MM tumor growth. We identified interleukin-6 (IL-6) readily increases PBK expression. Kaplan-Meier analysis showed the patients with higher expression have a significant shorter survival time compared those moderate/lower PBK. Knockout dramatically suppressed vivo growth cells, while genome editing changing...
Malignant mesothelioma (MMe) is a rare but aggressive malignancy. Although the molecular genetics of MMe known, including BRCA1-associated protein-1 (BAP1) gene alterations, prognosis patients remains poor. Here, we generated BAP1 knockout (BAP1-KO) human mesothelial cell clones to develop molecular-targeted therapeutics based on genetic alterations in MMe. cDNA microarray and quantitative RT-PCR (qRT-PCR) analyses revealed high expression calcium/calmodulin-dependent protein kinase type II...
CRISPR/Cas9 is a powerful genome editing system that has remarkably facilitated gene knockout and targeted knock-in. To accelerate the practical use of CRISPR/Cas9, however, it remains crucial to improve efficiency, precision, specificity editing, particularly knock-in, achieved with this system. researchers should first have molecular assay allows sensitive monitoring events simple procedures. In current study, we demonstrate occurring in L1CAM, an X-chromosome encoding cell surface...
Abstract Tandem paired nicking (TPN) is a method of genome editing that enables precise and relatively efficient targeted knock-in without appreciable restraint by p53-mediated DNA damage response. TPN initiated introducing two site-specific nicks on the same strand using Cas9 nickases in such way encompass site are located within homologous region between donor genome. This design results creation genome, leading to homology-directed recombination these fragments. In this study, we sought...
There has been a great deal of research on cell division and its mechanisms; however, processes still have many unknowns. To find novel proteins that regulate division, we performed the screening using siRNAs and/or expression plasmid target genes identified leucine zipper protein 1 (LUZP1). Recent studies shown LUZP1 interacts with various stabilizes actin cytoskeleton; function in mitosis is not known. In this study, found colocalized chromosomal passenger complex (CPC) at centromere...
In this study, an on-body Planar Inverted-F Antenna (PIFA) has been designed for biomedical purposes operated in Medical Implant Communication Service (MICS) (401-406MHz) band. The primary reasons behind choosing MICS band are its only application is found medical sector due to world wide availability, acceptable propagation and characteristics. main features of antenna minimized sized compare than recent antennas. total dimension 29mm×29mm×1.04mm. order make bio medically suitable use on...
PDZ-binding kinase (PBK) has been reported as a poor prognostic factor and is promising molecular target for anticancer therapeutics. Here, we aimed to investigate the effect of specific PBK inhibitor OTS514 on survival OSCC cells.
Abstract Malignant pleural mesothelioma (MPM) is an uncommon yet deadly cancer linked to asbestos exposure. The lack of effective early diagnosis and treatment leads reduced life expectancy among patients with MPM. This study aimed identify a novel molecular target inhibitor develop more therapeutics for Our drug screening assay showed that the fatty acid synthase (FASN) cerulenin demonstrates strong selective anti-proliferative properties against NF2/CDKN2A(p16) -deficient MPM cells,...
Abstract ZNHIT3 (zinc finger HIT type containing protein 3) is an evolutionarily conserved required for ribosome biogenesis by mediating the assembly of small nucleolar RNAs (snoRNAs) class C/D into ribonucleoprotein complexes (snoRNPs). Missense mutations in gene encoding have been previously reported to cause PEHO syndrome, a severe neurodevelopmental disorder typically presenting after birth. We discuss here case two fetuses from single family who presented with isolated hydrops during...