A5003623560- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Cancer-related Molecular Pathways
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Ocular Diseases and Behçet’s Syndrome
- Systemic Lupus Erythematosus Research
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Reproductive tract infections research
- Lung Cancer Research Studies
- Cancer Research and Treatments
- Viral Infections and Immunology Research
- Vasculitis and related conditions
- Gestational Trophoblastic Disease Studies
- Virology and Viral Diseases
- Immunodeficiency and Autoimmune Disorders
- Blood disorders and treatments
- Oral Health Pathology and Treatment
- Genetic Mapping and Diversity in Plants and Animals
The University of Sydney
2023
Griffith University
2014-2023
Royal Hobart Hospital
2023
Peter Doherty Institute
2023
The University of Melbourne
2023
RMIT University
2023
University of the Sunshine Coast
2023
Ecosystem Sciences
2023
University of New England
2023
Sheffield Hallam University
2023
MUC13 is a transmembrane mucin glycoprotein that over produced by many cancers, although its functions are not fully understood. Nuclear factor-κB (NF-κB) key transcription factor promoting cancer cell survival, but therapeutically targeting this pathway has proved difficult because NF-κB pleiotropic functions. Here, we report prevents colorectal death two distinct pathways of NF-kB activation, consequently upregulating BCL-XL. promoted tumor necrosis (TNF)-induced activation interacting...
RNA interference (RNAi) is an emerging and promising therapy for a wide range of respiratory viral infections. This highly specific suppression can be achieved by the introduction short-interfering (siRNA) into mammalian systems, resulting in effective reduction load. Unfortunately, this has been hindered lack good delivery system, especially via intranasal (IN) route. Here, we have developed IN siRNA encapsulated lipid nanoparticle (LNP) vivo system that efficient at targeting severe acute...
Gibbon ape leukaemia virus (GALV) and koala retrovirus (KoRV) share a remarkably close sequence identity despite the fact that they occur in distantly related mammals on different continents. It has previously been suggested infection of their respective hosts may have occurred as result species jump from another, yet unidentified vertebrate host. To investigate possible sources these retroviruses Australian context, DNA samples were obtained 42 screened using PCR order to detect proviral...
OBJECTIVES--To assess the incidence and significance of anticardiolipin antibodies after myocardial infarction in unstable angina. DESIGN--A prospective study all patients under 60 admitted to coronary care unit over a 12 month period with diagnosis acute who were followed up for further months. Patients angina similarly assessed but not up. Anticardiolipin antibody concentrations compared those age matched controls. SETTING--A district general hospital. PATIENTS--307 160...
Human papillomavirus (HPV) is the causative agent in cervical cancer. HPV oncogenes are major drivers of transformed phenotype, and cancers remain addicted to these oncogenes. A screen human kinome has identified inhibition Aurora kinase (AURKA) as being synthetically lethal on background E7 expression. The investigational AURKA inhibitor MLN8237/Alisertib selectively promoted apoptosis cancers. was driven by an extended mitotic delay Alisertib-treated E7-expressing cells. This had effect...
There is a dramatic rise in the incidence of Human papillomavirus (HPV) - associated head and neck squamous cell carcinoma (HNSCC) world, with considerable variation by geography, gender ethnicity. Little known about situation Bangladesh, where tobacco- areca nut-related cancers (HNCs) are most common men. We aimed to determine prevalence HPV HNSCC Bangladesh explore possible value cycle markers clinical diagnostic settings.One hundred ninety six archival tissue samples were analysed for...
Liposomes are versatile and well-proven as a means to deliver nucleic acids into cells. Most of the formulation procedures used labour intensive result in unstable end products. We have previously reported on development simple, yet efficient, hydration-of-freeze-dried-matrix (HFDM) method entrap siRNA within lipid particles. Here we show that particles stable up 12 months after storage at room temperature (RT), 4°C or -20°C. While RT results changes particle size polydispersity, gene...
Human metapneumovirus (hMPV) is an important human respiratory pathogen and implicated in array of illnesses, ranging from asymptomatic infection to severe bronchiolitis. Currently, there no reliable vaccine or specific antiviral therapy for hMPV treatment supportive. The use ribonucleic acid interference has the potential change that with targeting essential viral genes via small interfering RNAs (siRNAs) offering ability directly rapidly treat infections.The lung carcinoma epithelial cell...
The prevalence of koala retrovirus (KoRV) provirus (DNA) and the average number proviral insertions per cell vary in different free-ranging (Phascolarctos cinereus) populations across Australia. Populations northern states Queensland New South Wales have 100% mean copy 140–165 cell. In contrast, southern Victoria Australia differs among populations, with a these states’ mainland 73% 38%, respectively on island ranging from 0–50%. load is comparatively low, some having an less than 1 KoRV RNA...
Human papilloma virus (HPV) is the main culprit in cervical cancers. Although HPV vaccine now available, slow and gradual process for cancers to form means little will change, even vaccinated individuals. This warrants development of new therapeutic strategies both newly diagnosed recurrent patients. We have previously shown that Alisertib (MLN8237), an Aurora A kinase inhibitor, potently selectively kills HPV-positive cancer cells. However, known its unfavorable side effects when...
Context: In a global environment of escalating antimicrobial resistance and limited new antibiotic development, there is desire to optimize use across all settings preserve options for the future. Despite this, antibiotics continue be prescribed inappropriately in many settings. Patients with spinal cord injury (SCI) are particularly vulnerable receiving multiple courses antibiotics, yet data reviewing stewardship (AMS) programs this patient population.Hypothesis: Adopting AMS rounds will...
The genome of koala retrovirus (KoRV) has striking similarity to the gibbon ape leukemia virus (GALV) genome, suggesting two viruses may share a common ancestor. Screening DNA from range potential hosts this putative ancestor revealed retroviral sequence grassland melomys (Melomys burtoni) that was closely related both KoRV and GALV. This novel been named Melomys burtoni (MbRV). As koalas habitat, it is possible there cross-species transmission in past. Although causative relationship...
Abstract MUC13 is a transmembrane mucin glycoprotein that overexpressed by many cancers, although its functions are not fully understood. NF-κB key transcription factor promoting cancer cell survival, but therapeutically targeting this pathway has proved difficult because pleiotropic functions. Here, we report prevents colorectal death two distinct pathways of NF-kB activation, consequently up-regulating BCL-XL. promoted TNF-induced activation interacting with TNFR1 and the E3 ligase, cIAP1,...
Many viruses have evolved mechanisms to antagonize the interferon (IFN) system, targeting all major components involved in receptor binding and signaling. Although a number of these vital proteins are homologous cellular IFN downregulation (e.g., viral regulatory factors [vIRFs]), many share little resemblance known proteins. To determine IFN-blocking properties proteins, functional assays required. Here, we present new rapid screening method, based on 2fTGH cell line, which is able gene...
Abstract RNA interference (RNAi) is an emerging and promising therapy for a wide range of respiratory viral infections. This highly specific suppression can be achieved by the introduction short-interfering (siRNA) into mammalian systems, resulting in effective reduction load. Unfortunately, this has been hindered lack good delivery system, especially via intranasal (IN) route. Here, we have developed IN siRNA encapsulated lipid nanoparticle (LNP) vivo system that efficient at targeting...
In the second semester of 2020, despite need to pivot online learning in response COVID-19 pandemic, our academic teaching team commenced delivery a brand-new, bespoke parasitology subject third year Bachelor Biomedicine and Science students at The University Melbourne. This purposefully designed subject, ‘Medical Microbiology: Parasitology’, was developed collaboration with internationally renowned parasitologists global health researchers take broad, multi-disciplinary approach student...
The Sustainable Development Goals (SDGs) adopted by the United Nations in 2015 constitute a 'universal call to action end poverty, protect planet, and ensure that 2030 all people enjoy peace prosperity'.The 17 SDGs are integrated target development of 'balance social, economic environmental sustainability' recognise 'action one area will affect outcomes others'.'The creativity, knowhow, technology financial resources' from every sector society required for timely delivery SDGs.The major...