A5084085223- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Herpesvirus Infections and Treatments
- Livestock and Poultry Management
- Virus-based gene therapy research
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Vector-Borne Animal Diseases
- Livestock Farming and Management
- Microbial infections and disease research
- Rabies epidemiology and control
- Plant Virus Research Studies
- Parvovirus B19 Infection Studies
- Zoonotic diseases and public health
- Cytomegalovirus and herpesvirus research
- Genetic and phenotypic traits in livestock
- Molecular Biology Techniques and Applications
- Animal Nutrition and Physiology
- Bat Biology and Ecology Studies
- Rabbits: Nutrition, Reproduction, Health
- Animal Genetics and Reproduction
- Agricultural Systems and Practices
The University of Queensland
2014-2023
Platinum Group Coatings (United States)
2021
Australian Centre for Disease Preparedness
2015
Commonwealth Scientific and Industrial Research Organisation
2015
Center for Indonesian Veterinary Analytical Studies
2015
National Institute of Veterinary Research
2015
Cape Town Science Centre
2006
Massey University
1997-2002
University of Veterinary Medicine in Košice
1998
Murdoch University
1992-1995
SUMMARY: Ammonia-limited Aerobacter aerogenes, Erwinia carotovora, Pseudomonas fluorescens, Bacillus subtilis and B. megaterium synthesized glutamate from NH3 2-oxoglutarate by a process that involved first the synthesis of glutamine then reductive transfer amide-nitrogen to 2-position 2-oxoglutarate. The latter step required recently reported enzyme ‘glutamine(amide): amino-transferase oxido-reductase (NADP)', some further properties which are described here. This enzyme, different...
Research Article| April 01 1970 Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route D W Tempest; Tempest Search for other works this author on: This Site PubMed Google Scholar J L Meers; Meers C M Brown Biochem (1970) 117 (2): 405–407. https://doi.org/10.1042/bj1170405 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn MailTo Cite Get Permissions Citation Tempest, Meers, Brown; route. 1 1970; doi:...
SUMMARY: The free amino acid pool contents of Gram-negative bacteria (Aerobacter aerogenes, Erwinia carotovora, Pseudomonas fluorescens) were studied as functions the growth environment and compared with those from correspondingly grown cultures Gram-positive (Bacillus subtilis var. niger, B. megaterium, polymyxa) yeast Saccharomyces cerevisiae. Although pools contained five to 20 times concentration acids present in bacteria, all similar containing only a limited range detectable acids....
Koala retrovirus (KoRV) is a newly described endogenous and unusual in that inserts comprise full-length replication competent genome. As koalas are known to suffer from an extremely high incidence of leukaemia/lymphoma, the association between this disease was examined. Using quantitative real-time reverse transcriptase PCR it demonstrated KoRV RNA levels plasma significantly increased animals suffering leukaemia or lymphoma when compared with healthy animals. Increased were also seen for...
Objective To determine the prevalence of koala retrovirus ( KoRV ) in selected populations and to estimate proviral copy number a subset koalas. Methods Blood or tissue samples from 708 koalas Q ueensland, N ew S outh W ales, V ictoria A ustralia were tested for pol provirus gene using standard polymerase chain reaction PCR ), nested real‐time qPCR ). Results Prevalence provirus‐positive was 100% four regions ueensland 72.2% mainland ictoria, 26.6% on ictorian islands 14.8% K angaroo I...
This study investigated the seroepidemiology of Hendra virus in a spectacled flying-fox (Pteropus conspicillatus) population northern Australia, near location an equine and associated human infection late 2004. The pattern was using serial cross-sectional serological over 25-month period, with blood sampled from 521 individuals six sampling sessions. Antibody titres to were determined by neutralisation test. In contrast expected episodic pattern, we observed that seroprevalence gradually...
Nipah virus (NiV) (Genus Henipavirus) is a recently emerged zoonotic that causes severe disease in humans and has been found bats of the genus Pteropus. Whilst NiV not detected Australia, evidence for NiV-infection pteropid some Australia's closest neighbours. The aim this study was to determine occurrence henipaviruses fruit bat (Family Pteropodidae) populations north Australia. In particular we tested hypothesis restricted west Wallace's Line. Fruit from Papua New Guinea, East Timor...
Significance Endogenous retroviruses (ERVs) are proviral sequences that result from host germ-line invasion by exogenous retroviruses. The majority of ERVs degraded. Using the koala retrovirus (KoRV) as a model system, we demonstrate recombination with an ancient retroelement disables KoRV, and occurs frequently early in process. Recombinant KoRVs (recKoRVs) then able to proliferate germ line. This may part explain generally degraded nature vertebrate genomes suggests degradation via is one...
Koala populations are in serious decline across many areas of mainland Australia, with infectious disease a contributing factor. retrovirus (KoRV) is gammaretrovirus present most wild koala and captive colonies. Five subtypes KoRV (A to E) have been identified based on amino acid sequence divergence hypervariable region the receptor binding domain envelope protein. However, analysis viral genetic diversity has conducted primarily koalas housed zoos Japan, United States, Germany. Wild within...
Following the SARS outbreak, extensive surveillance was undertaken globally to detect and identify coronavirus diversity in bats. This study sought prevalence of coronaviruses bats Australasian region. We identified four different genotypes coronavirus, three which (an alphacoronavirus two betacoronaviruses) are potentially new species, having less than 90% nucleotide sequence identity with most closely related described viruses. did not any SARS-like betacoronaviruses, despite targeting...
A study was conducted to investigate the persistence of rabbit haemorrhagic disease virus (RHDV) in environment. Virus impregnated onto two carrier materials (cotton tape and bovine liver) exposed environmental conditions on pasture during autumn New Zealand. Samples were collected after 1, 10, 44 91 days viability determined by oral inoculation susceptible 11- 14-week-old Zealand White rabbits. Evidence RHDV infection based clinical pathological signs and/or seroconversion RHDV. cotton...
Objective To determine the genetic variants of canine parvovirus‐2 (CPV) present in domestic dogs Australia and to investigate 26 cases apparent vaccine failure. Design Thirty‐three samples faeces or intestinal tissues 16 cell culture virus isolates collected over a period from 1980 2005 five Australian states were analysed. Procedure DNA was extracted 1975 bp fragment VP1/2 gene CPV amplified by polymerase chain reaction (PCR) sequenced. Sequences compared published strains CPV‐2, CPV‐2a,...
Abstract In Java, Indonesia, during March 2007–March 2008, 96 farms with scavenging ducks that were not vaccinated against highly pathogenic avian influenza (HPAI) monitored bimonthly. Bird-level (prevalence among individual birds) H5 seroprevalence was 2.6% for and 0.5% chickens in contact ducks. At least 1 seropositive bird detected 19.5% 2.0% of duck- chicken-flock visits, respectively. Duck flocks 12.4× more likely than chicken to have birds. During 21.4% farm ≥1 sampled duck when all...
SUMMARY The influence of Mg2+-limitation on the growth a typical Gram-positive organism—Bacillus subtilis—was investigated and data compared with that obtained Aerobacter aerogenes grown under similar conditions. magnesium contents both organisms varied rate but were very at corresponding rates. With Mg2+-limited chemostat cultures each organism, uptake Mg2+ was almost complete specific rates less than 0·7 × maximum. Cellular tightly bound, none being removed by suspension 20° in 0·85% (w/v)...
SUMMARY: Magnesium-limited mixed cultures, each containing two microbial species, were produced by exchange of small numbers organisms between magnesium-limited chemostat cultures Aerobacter aerogenes, Pseudomonas fluorescens, Bacillus subtilis, B. megaterium, Staphylococcus epidermis and Torula utilis. The fate species in these was determined following cross-inoculation. Gram-negative invariably outgrew the Gram-positive ones, but ability subtilis megaterium to outgrow other, or yeast,...
Gibbon ape leukaemia virus (GALV) and koala retrovirus (KoRV) share a remarkably close sequence identity despite the fact that they occur in distantly related mammals on different continents. It has previously been suggested infection of their respective hosts may have occurred as result species jump from another, yet unidentified vertebrate host. To investigate possible sources these retroviruses Australian context, DNA samples were obtained 42 screened using PCR order to detect proviral...
Feline panleukopenia (FPL), a frequently fatal disease of cats, is caused by feline parvovirus (FPV) or canine (CPV). We investigated simultaneous outbreaks FPL between 2014 and 2018 in Australia, New Zealand the United Arab Emirates (UAE) where had not been reported for several decades. Case data from 989 cats clinical samples additional 113 were obtained to determine cause epidemiological factors involved. Most with shelter-housed, 9 10 weeks old at diagnosis, unvaccinated, completed...