The combination of ascorbic acid and rutin is frequently used in oral preparations. However, despite numerous protective effects each component individually, their combined effect on ultraviolet (UV)-irradiated skin cells has never been evaluated. aim this study was to evaluate the human keratinocytes fibroblasts exposed UVA UVB radiation. Skin radiation were treated with or/and rutin. total antioxidant properties both components, as well cellular pro- status, lipid protein oxidation,...

Several epidemiological studies propose the association of rheumatoid arthritis (RA) with oxidative stress. The aim this study was to estimate possible onset systemic lipid peroxidation in RA patients and its relevance for pathophysiology monitoring RA. Seventy-three 73 healthy subjects were included study. Lipid estimated by measurement 4-hydroxynonenal (4-HNE), 4-hydroxyhexenal, malondialdehyde, acrolein, crotonaldehyde, 4-oxononenal, isoprostanes (8-isoPGF2α) levels. Cytosolic...

Psoriasis is a chronic inflammatory skin disease characterized by dysregulated keratinocyte differentiation, but oxidative stress also plays an important role in the pathogenesis of this disease. Here, we examined effect cannabidiol (CBD), phytocannabinoid with antioxidant and anti-inflammatory properties, on redox balance phospholipid metabolism UVA/UVB-irradiated keratinocytes isolated from psoriatic patients or healthy volunteers. CBD accumulates mainly membrane keratinocytes, especially...

We investigated the influence of cannabidiol (CBD) on blood pressure (BP) and heart rate (HR) in spontaneously (SHR) deoxycorticosterone (DOCA-salt) hypertensive rats. Hypertension was connected with increases cardiac plasma markers lipid peroxidation both models, whereas endocannabinoid levels decreased SHR increased DOCA-salt. CBD (10 mg/kg once a day for 2 weeks) did not modify BP HR hypertension but counteracted pro-oxidant effects. Moreover, it or anandamide, 2-arachidonoylglycerol...

Several studies suggested the association of COVID-19 with systemic oxidative stress, in particular lipid peroxidation and vascular stress. Therefore, this study aimed to evaluate antioxidant signaling plasma eighty-eight patients upon admission Clinical Hospital Dubrava Zagreb, which twenty-two died within a week, while other recovered. The differences between deceased survivors were found, especially reduction superoxide dismutases (SOD-1 SOD-2) activity, was accompanied by alteration...

Lipids and proteins of skin cells are the most exposed to harmful ultraviolet (UV) radiation contained in sunlight. There is a growing need for natural compounds that will protect these sensitive molecules from damage, without side effects. The aim this study was investigate effect sea buckthorn seed oil on redox balance lipid metabolism UV irradiated formed different layers examine whether it had protective effect. Human keratinocytes fibroblasts were subjected UVA (ultraviolet type A; 30...

The combination of ascorbic acid and rutin, commonly used in oral preparations for their antioxidant anti-inflammatory properties, can also be to protect skin cells from the effects UV radiation sunlight. Here, we tested potential protective effect rutin together UVB-irradiated human fibroblasts, assessed proteomic profile these cells, grown a three-dimensional (3D) system. Proteomic findings revealed combined UV-irradiated fibroblasts against overexpression pro-inflammatory signaling...

There is a great need for compounds with antioxidant and anti‐inflammatory properties protection against UV radiation, which the most prooxidative physical factor that skin cells are exposed to everyday. Therefore, aim of study was evaluate mechanism phytocannabinoid‐cannabidiol (CBD) action in vivo on lipid metabolism keratinocytes rat UVA/UVB radiation. Our results show CBD protects effects radiation by reducing peroxidation products: 4‐HNE 8‐isoPGF 2 α . In addition, significantly...

Pulmonary arterial hypertension (PAH) is a rare multifactorial disease with an unfavorable prognosis. Sildenafil therapy can improve functional capacity and pulmonary hemodynamics in PAH patients. Nowadays, it increasingly recognized that the effects of sildenafil are pleiotropic may also involve changes pro-/antioxidant balance, lipid peroxidation autonomic control. In present study we aimed to assess on fatty acids (FAs) status, level hydroxynonenal (HNE) heart rate variability (HRV)...

Background: The purpose of this study was to assess the processes lipid peroxidation with prostaglandin derivatives and reactive aldehydes being its major indicators in cerebrospinal fluid (CSF), plasma urine patients tick-borne encephalitis (TBE). Materials methods: This included 60 TBE 56 healthy subjects. Lipid estimated by measurement 4-hydroxynonenal (4-HNE), 4-hydroxyhexenal (4-HHE), malondialdehyde (MDA), acrolein, crotonaldehyde, 4-oxononenal (4-ONE), determined GC-MS,...

The combination of ascorbic acid and rutin, often used in oral preparations, due to antioxidant anti-inflammatory properties, can be protect skin cells against the effects UV radiation from sunlight. Therefore, aim this study was investigate synergistic effect rutin on proteomic profile UVA UVB irradiated keratinocytes cultured a three-dimensional (3D) system. Results showed that protects UV-induced changes. In particular, alterations were observed expression proteins involved response, DNA...

As a result of SARS-CoV-2 infection, inflammation develops, which promotes oxidative stress, leading to modification phospholipid metabolism. Therefore, the aim this study is compare effects COVID-19 on levels and free polyunsaturated fatty acids (PUFAs) their metabolites produced in response reactions with reactive oxygen species (ROS) enzymes (cyclooxygenases-(COXs) lipoxygenase-(LOX)) plasma patients who either recovered or passed away within week hospitalization. In patients, especially...

UVA exposure disturbs the metabolism of skin cells, often inducing oxidative stress and inflammation. Therefore, there is a need for bioactive compounds that limit such consequences without causing undesirable side effects. The aim this study was to analyse in vitro effects phytocannabinoids cannabigerol (CBG) cannabidiol (CBD), which differ terms biological Furthermore, combined use both (CBG+CBD) has been analysed order increase their effectiveness human fibroblasts keratinocytes...

Ticks are vectors of various pathogens, including tick-borne encephalitis virus and bacteria such as B. burgdorferi A. phagocytophilum, causing infections/co-infections, which still a diagnostic therapeutic problem. Therefore, the aim this study was to compare effects TBEV infection/bacterial co-infection on metabolic changes in blood patients before after treatment. It found that those infections promote plasma ROS enhanced generation antioxidant defence reduction, especially relation...

Excessive daily exposure of human skin to natural UVA radiation leads impaired redox homeostasis in epidermal keratinocytes, resulting changes their proteome. Commonly used antioxidants usually exhibit protection a narrowed range, which makes it necessary combine effects. Therefore, the aim this study was analyze protective effect cannabigerol (CBG) and 3-O-ethyl ascorbic acid (EAA), separately together, on proteomic profile irradiated keratinocytes. Proteomic analysis with use Q Exactive HF...

Psoriasis is accompanied by disturbed redox homeostasis, with systemic and local oxidative stress promoting the modification of basic components cellular membranes. Therefore, aim study was to investigate effect development psoriasis vulgaris psoriatic arthritis on composition physicochemical properties skin cell membranes (keratinocytes fibroblasts) blood cells (lymphocytes, granulocytes erythrocytes). Both forms are characterized decreased levels changes in localization membrane...

Phospholipids and their metabolites play an important role in maintaining the membrane integrity metabolic functions of keratinocytes under physiological conditions regeneration process after exposure to high-energy UVB radiation. Therefore, search for compounds with a protective regenerative effect on keratinocyte phospholipids, effectiveness two antioxidant has been tested: stable derivative ascorbic acid, 3-O-ethyl acid (EAA) cannabigerol (CBG), both which are primarily located structures...