Tuberculosis treatment consists of a fixed dose combination rifampicin (RIF), isoniazid (INH), pyrazinamide (PYZ), and ethambutol hydrochloride (EMB). The combined using various drugs is necessary for patient curing, without recrudescence, prevention drug-resistant mutants, which may occur during treatment. An HPLC-diode array detector (DAD) method the simultaneous determination RIF, INH, PYZ, EMB in tablets was developed validated. Chromatographic experiments were performed on an Agilent...

A validated sub minute capillary zone electrophoresis method with direct ultraviolet absorption detection for simultaneous determination of isoniazid and rifampicin in fixed-dose combination tablets was developed. Background electrolyte defined based on the analytes effective mobility curve it composed by 20 mmol/L sodium carbonate/sodium bicarbonate at pH 10.2. careful validation procedure considering main figures merit performed. Regression models were satisfactory rifampicin, showing no...

The present study developed and validated an HPLC method for the simultaneous determination of artesunate (AS) mefloquine hydrochloride (MQ) in fixed-dose combination tablets, according to ICH guidelines. chromatographic separation was carried out on XBridge C18 (250 x 4.6 mm i.d., 5 µm particle size, Waters) analytical column. mobile phase included a 0.05 M monobasic potassium phosphate buffer (pH adjusted 3.0 with phosphoric acid) acetonitrile (50 + 50, v/v). flow rate 1.0 mL/min, run time...

The potential of capillary electrophoresis was demonstrated to the determination fatty acids in food and cosmetic samples a validated method is proposed palmitic, stearic, oleic linoleic quantification these samples.

The presence of many biologically active components makes pumpkins extremely attractive to the phytochemical manufacturing industry. Studies have demonstrated that oil extracted from seeds has different biological activities. This study aimed determine fatty acid composition and total tocopherol content pumpkin seed (Cucurbita moschata – Jacarezinho cultivar) by supercritical carbon dioxide (Sc-CO2) hexane/isopropanol. acids tocopherols were determined GC, GC/FID, HPLC, respectively. We also...

In this study, herbal tinctures prescribed by the Brazilian public health system were analyzed for their Cd and Cr concentrations direct analysis using electrothermal graphite furnace atomic absorption spectrometry (GF-AAS). The acidic decomposition assisted microwave radiation was employed to evaluate efficiency of proposed method, demonstrating no significant differences between methods. accuracy method evaluated obtaining adequate recoveries (106 ± 4) % (108 Cd, as well precision...

A novel method was proposed for simultaneous determination of artesunate (ATS) and mefloquine (MFQ) in fixed-dose combination tablets by capillary zone electrophoresis with direct indirect detection ultraviolet (CZE-UV).

Experimental design as an optimization protocol of mass spectrometry parameters towards the acquisition high quality datasets for metabolomics-driven investigations.

Tuberculosis is the second most deadly infectiousdisease, surpassed only by HIV/AIDS, and hasresulted in over 1 billion deaths last200 years. The World Health Organization estimatesthat 2014, 9.6 million people were infected bythis disease 1.5 had died. First-choicetreatment consists of fixed-dose combinationtablets containing rifampicin, isoniazid,pyrazinamide, ethambutol hydrochloride(4-FDC). There are pharmacopeial protocolsavailable to test 4-FDC, but they prolonged,two-step methods. One...

Abstract Tuberculosis is the second most deadly infectious disease, surpassed only by HIV/AIDS, and has resulted in over 1 billion deaths last 200 years. The World Health Organization estimates that 2014, 9.6 million people were infected this disease 1.5 had died. First-choice treatment consists of fixed-dose combination tablets containing rifampicin, isoniazid, pyrazinamide, ethambutol hydrochloride (4-FDC). There are pharmacopeial protocols available to test 4-FDC, but they prolonged,...

DIAGNOSTIC TESTS FOR SARS-COV-2: A CRITICAL REFLECTION. The new coronavirus, called Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), was discovered in late December 2019 after cases were reported the city of Wuhan, China. In January 2020, World Health Organization (WHO) officially declared Disease (COVID-19) as a pandemic, which has an official record around 500 million and more than 6.0 deaths worldwide. An important factor controlling pandemic is development effective...

Capillary electrophoresis (CE) is an electromigration-based separation technique that emerged in the early 90s and has been evolving since then. Over recent decades, association of CE with new technologies detections strategies enhancing its intrinsic mightiness. Today, a part main high-throughput analytical platform used for medical-related applications, together traditional gas chromatography, high-performance liquid fingerprinting spectroscopic methods. New assessing human disorders...

ABSTRACT Novel methods were proposed for determining primaquine (PQN) in tablets by multiple‐injection capillary zone electrophoresis (MI‐CZE) and short‐end injection CZE (SEI‐CZE), both with ultraviolet detection. The background electrolyte (BGE), consisting of 20 mmol/L tris (hydroxymethyl) aminomethane 30 hydrochloric acid at pH 2.0, was selected considering a comprehensive study involving the effective mobility versus curves analytes BGE components. Experimental designs applied...

Trichomoniasis is the most common non-viral sexually transmitted infection (STI) in world. It caused by protozoan Trichomonas vaginalis, and infections are asymptomatic – making difficult diagnosis allowing parasite to spread. Due COVID19 pandemic 2020, social isolation measures were taken prevent transmission of SARS-Cov-2. Interestingly, some studies have shown an increase number STIs during after lockdown ended. In this context, we evaluated impact that had on percentage positive urine...