A5051371499- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Nicotinic Acetylcholine Receptors Study
- Sleep and Wakefulness Research
- Neuroscience of respiration and sleep
- Neuroendocrine regulation and behavior
- Infant Health and Development
- Epilepsy research and treatment
- Pain Mechanisms and Treatments
- Diffusion and Search Dynamics
- Receptor Mechanisms and Signaling
- Cardiac electrophysiology and arrhythmias
- Botulinum Toxin and Related Neurological Disorders
- Ion Transport and Channel Regulation
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- RNA regulation and disease
- EEG and Brain-Computer Interfaces
- Fuzzy Logic and Control Systems
- Magnesium in Health and Disease
- Neural Networks and Applications
- Microtubule and mitosis dynamics
Sorbonne Université
2008-2022
Institut de Biologie Paris-Seine
2016-2022
Centre National de la Recherche Scientifique
2008-2022
Inserm
2014-2022
Neurosciences Paris-Seine
2014-2022
Université Paris-Seine
2015-2018
Gènes, synapses et cognition
2017
Centre National pour la Recherche Scientifique et Technique (CNRST)
2013
Université Paris Cité
2008-2011
Cardiff University
2011
Slow waves represent one of the prominent EEG signatures non-rapid eye movement (non-REM) sleep and are thought to play an important role in cellular network plasticity that occurs during this behavioral state. These slow natural currently considered be exclusively generated by intrinsic synaptic mechanisms within neocortical territories, although a for thalamus key physiological rhythm has been suggested but never demonstrated. Combining neuronal ensemble recordings, microdialysis,...
1. Intracellular Ca2+ concentration ([Ca2+]i) was monitored in single cells isolated from adult rat supraoptic (SO) nuclei. The great majority of (85%) were neurones and most immunoreactive to oxytocin or vasopressin (AVP). 2. resting [Ca2+]i the (80%) remained stable while 20% displayed spontaneous oscillations which disappeared low‐Ca2+ (100 nM) EGTA buffer. 3. Addition 100 nM increased both oscillating cells. Two types responses observed: (i) a sustained response with being maintained at...
Although it is well established that low-voltage-activated T-type Ca 2+ channels play a key role in many neurophysiological functions and pathological states, the lack of selective potent antagonists has so far hampered detailed analysis full impact these might have on single-cell neuronal network excitability as homeostasis. Recently, novel series piperidine-based molecules been shown to selectively block recombinant but not high-voltage-activated (HVA) affect number physiological...
The thalamic output during different behavioral states is strictly controlled by the firing modes of thalamocortical neurons. During sleep, their hyperpolarized membrane potential allows activation T-type calcium channels, promoting rhythmic high-frequency burst that reduces sensory information transfer. In contrast, in waking state neurons mostly exhibit action potentials at low frequency (i.e., tonic firing), enabling reliable transfer incoming inputs to cortex. Because nearly complete...
Initial anatomical and physiological studies suggested that sensory information relayed from the periphery by thalamus is serially processed in primary cortical areas. It thought to propagate layer 4 (L4) up L2/3 down L5, which constitutes main output of cortex. However, more recent experiments point toward existence a direct processing thalamic input L5 neurons. Therefore, role neurons operated now highly debated. Using cell type-specific reversible optogenetic manipulations somatosensory...
At the molecular level, our knowledge of low voltage-activated Ca 2+ channel (T-type) has made little progress. Using an antisense strategy, we investigated possibility that T-type channels have a structure similar to high channels. It is assumed are at least three components: pore forming α 1 subunit combined with cytoplasmic modulatory β and primarily extracellular 2 δ subunit. We examined effect transfecting cranial primary sensory neurons generic anti-β oligonucleotides. show in this...
T-type calcium channel isoforms expressed in heterologous systems demonstrate marked differences the biophysical properties of resulting currents. Such heterogeneity gating behaviour not only reflects structural but is also observed following regulation activity by a number ligands. However, physiological impact these parameters T channels has never been evaluated situ where unique interplay between and other intrinsic currents conserved, activation can be triggered synaptic stimulation....
Abstract Voltage‐dependent activity around the resting potential is determinant in neuronal physiology and participates definition of firing pattern. Low‐voltage‐activated T‐type Ca 2 + channels directly affect membrane control a number secondary ‐dependent permeabilities. We have studied ability cloned (α1G,H,I) to carry currents response mock action potentials. The relationship between spike duration current amplitude specific for each channels, reflecting their individual kinetic...
The formation of a biological seal around the transmucosal portion dental implants may be crucial for long‐term success these therapies. Data to date suggest that gingival epithelium attaches through hemidesmosomes. Biochemical and genetic data indicate laminin isoform, laminin‐5, component basement membranes, plays role in assembly maintenance We report use soluble laminin‐5 as coating titanium‐alloy promote cell attachment epithelial line, IHGK. Monoclonal antibodies reactive with depleted...
The GABAergic neurons of the nucleus reticularis thalami that control communication between thalamus and cortex are interconnected not only through axo-dendritic synapses but also gap junctions dendro-dendritic synapses. It is still unknown whether these dendritic processes may be triggered both by tonic T-type Ca(2+) channel-dependent high frequency burst firing action potentials displayed during wakefulness sleep, respectively. Indeed, while it known activation channels actively propagates...
Recently, two members of a new family Ca2+ channel alpha1 subunits, alpha1G (or CavT.1) and alpha1H CavT.2), have been cloned expressed. These subunits generate Ba2+ currents similar to the T-type present in sensory neurons. Here, we use three methods investigate whether T nodosus ganglion neurons are encoded by CavT family. PCR detected presence mRNA encoding both alpha1H, as well third highly related sequence, alpha1I. In situ hybridizations performed on ganglia demonstrate high expression...
Slow waves of non-REM sleep are suggested to play a role in shaping synaptic connectivity consolidate recently acquired memories and/or restore homeostasis. During slow waves, both GABAergic neurons the nucleus reticularis thalami (NRT) and thalamocortical (TC) discharge high-frequency bursts action potentials mediated by low-threshold calcium spikes due T-type Ca 2+ channel activation. Although such activity intrathalamic network characterized firing influx is highly suited modify efficacy,...