A5021910052- Electron and X-Ray Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Ion-surface interactions and analysis
- Advanced Materials Characterization Techniques
- Advanced X-ray Imaging Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Cryospheric studies and observations
- Dendrimers and Hyperbranched Polymers
- nanoparticles nucleation surface interactions
- Advanced Polymer Synthesis and Characterization
- Climate change and permafrost
- Arctic and Antarctic ice dynamics
- Plant Disease Resistance and Genetics
- Geological and Geochemical Analysis
- High-pressure geophysics and materials
- Advancements in Photolithography Techniques
Thermo Fisher Scientific (Netherlands)
2010
Thermo Fisher Scientific (Japan)
2010
Summary The combination of focused ion beam and scanning electron microscopy with a cryo‐preparation/transfer system allows specimens to be milled at low temperatures. However, for biological in particular, the quality results is strongly dependent on correct preparation specimen surface. We demonstrate method deposition protective, planarizing surface layer onto cryo‐sample, enabling high‐quality cross‐sectioning using investigation structures nanoscale.
The introduction of cryo-techniques to the focused ion-beam scanning electron microscope (FIB-SEM) has brought new opportunities study frozen, hydrated samples from field Life Sciences. Cryo-techniques have long been employed in microscopy. Thin transparent sections are produced by cryo-ultramicrotomy for observation a cryo-transmission (TEM). Cryo-TEM is presently reaching imaging macromolecular structures. In parallel, cryo-fractured surfaces bulk materials investigated cryo-SEM. Both...
Abstract The structure of brine films in grain boundaries halite has been the subject much controversy over past 20 years; although a number innovative methods have developed to study these structures, is still unknown and fundamental information missing. In this study, we investigated different plunge‐freezing vitrify fill for natural salt polycrystal. This was followed by preliminary 3D morphology vitrified boundary rock sample with focused ion beam (FIB) excavation system. We shown that...
Naturally deformed ice contains subgrains with characteristic geometries that have recently been identified in etched surfaces using high-resolution light microscopy (LM). The probable slip systems responsible for these subgrain boundary types can be determined electron backscattered diffraction (EBSD), providing the etch features imaged reflected LM retained during EBSD data acquisition a scanning microscope (SEM). Retention of requires surface is stable. Depending on pressure and...
Summary The desire to study macromolecular complexes within their cellular context requires the ability produce thin samples suitable for cryo‐TEM (cryo‐transmission electron microscope) investigations. In this paper, we discuss two similar approaches, which were developed independently in Utrecht (the Netherlands) and Albany (USA). methods are particularly both tissue cell suspensions prepared by a high‐pressure freezer (HPF). workflows explained with particular attention potential...
Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – 5, 2010.
Extract Extended abstract of a paper presented at Microscopy and Microanalysis 2007 in Ft. Lauderdale, Florida, USA, August 5 – 9,
Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.
Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – 8, 2013.
Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – 5, 2010.