A5023139260- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Advanced Materials Characterization Techniques
- Hydrocarbon exploration and reservoir analysis
- Advanced X-ray Imaging Techniques
- Planarian Biology and Electrostimulation
- Ion channel regulation and function
- Dielectric materials and actuators
- Wound Healing and Treatments
- Geophysical and Geoelectrical Methods
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Algal biology and biofuel production
- Non-Destructive Testing Techniques
- Advanced battery technologies research
- Plant Water Relations and Carbon Dynamics
- Planetary Science and Exploration
- Robotics and Sensor-Based Localization
- Viral Infections and Outbreaks Research
- Supercapacitor Materials and Fabrication
- Viral gastroenteritis research and epidemiology
- Advanced Sensor and Energy Harvesting Materials
- Nuclear Physics and Applications
- Mitochondrial Function and Pathology
University of California, Santa Cruz
2024
Wadsworth Center
2002-2020
New York State Department of Health
1998-2020
Howard Hughes Medical Institute
2006
National Tsing Hua University
2001
Nippon Shokubai (Japan)
2001
East China University of Science and Technology
2001
Osaka National Hospital
2001
Kanazawa University
2001
Mitsubishi Materials (Japan)
2001
Summary The feasibility of using a focused ion beam (FIB) for the purpose thinning vitreously frozen biological specimens transmission electron microscopy (TEM) was explored. A concern whether heat transfer beyond direct interaction layer might devitrify ice. To test this possibility, we milled water on standard TEM grid with 30‐keV Ga + beam, and cryo‐transferred to examination. Following FIB milling vitreous ice from thickness approximately 1200 nm 200–150 nm, changes characteristic...
The architectural design of electrodes offers new opportunities for next-generation electrochemical energy storage devices (EESDs) by increasing surface area, thickness, and active materials mass loading while maintaining good ion diffusion through optimized electrode tortuosity. However, conventional thick increase length cause larger concentration gradients, limiting reaction kinetics. We demonstrate a strategy building interpenetrated structures that shortens reduces inhomogeneity. This...
Abstract The ability to deliver drugs with precise dosages at specific time points can significantly improve disease treatment while reducing side effects. Drug encapsulation for gradual delivery has opened the doors a superior regimen. To expand on this ability, programming bioelectronic devices small molecules enables ad‐hoc personalized therapeutic profiles that are more complex than release. Here, wearable device an integrated electrophoretic ion pump affords on‐demand drug dose control...
Abstract Manganese dioxide (MnO 2 ) deposition/dissolution (Mn 2+ /MnO chemistry, involving a two‐electron‐transfer process, holds promise for safe and eco‐friendly large‐scale energy storage. However, challenges like electrode/electrolyte interface environment fluctuations (H + H O activity), irreversible Mn degradation, limited understanding of degradation mechanisms hinder the reversibility conversion. This study demonstrates vanadyl/pervanadyl (VO /VO redox‐mediated designed high‐energy...
Electrical signaling plays a crucial role in the cellular response to tissue injury wound healing and an external electric field (EF) may expedite process. Here, we have developed standalone, wearable, programmable electronic device administer well-controlled exogenous EF, aiming accelerate vivo mouse model provide pre-clinical evidence. We monitored process by assessing re-epithelization rate ratio of M1/M2 macrophage phenotypes through histology staining. Following three days treatment,...
Summary The desire to study macromolecular complexes within their cellular context requires the ability produce thin samples suitable for cryo‐TEM (cryo‐transmission electron microscope) investigations. In this paper, we discuss two similar approaches, which were developed independently in Utrecht (the Netherlands) and Albany (USA). methods are particularly both tissue cell suspensions prepared by a high‐pressure freezer (HPF). workflows explained with particular attention potential...
Wound healing is a complex physiological process that requires precise control and modulation of many parameters. Therapeutic ion biomolecule delivery has the capability to regulate wound beneficially. However, achieving controlled through compact device with ability deliver multiple therapeutic species can be challenge. Bioelectronic devices have emerged as promising approach for delivery. Here, we present pro-reparative bioelectronic designed ions biomolecules applications. The...
Abstract Electron microscopic tomography is providing important new insights about the internal structure of mitochondrion. In particular, infoldings mitochondrial inner membrane (cristae), which are usually rendered as lamelliform baffles, revealed to have considerable tubular nature. Rather than opening wide peripheral compartment (between and outer membranes), cristae connect outside each other through narrow (20-30 nm) segments, can be hundreds nanometers long. This suggests that...
Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – 5, 2010.
Extract HTML view is not available for this content. However, as you have access to content, a full PDF via the ‘Save PDF’ action button. Extended abstract of paper presented at Microscopy and Microanalysis 2005 in Honolulu, Hawaii, USA, July 31--August 4,
Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – 7,
Abstract In skeletal muscle, depolarization of the plasma membrane, which is initiated at neuromuscular junction, transduced to a rise in cytoplasmic calcium specialized structures known as triad junctions (TJs). TJs occur myofiber’s interior regions near z-lines, where transversely oriented tubular invaginations membrane (T-tubules) form with two elements sarcoplasmic reticulum (SR). Isolation fractions that are enriched junctional complexes and retain function has been reported. Figure 1...
Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – 5, 2010.
Extended abstract of a paper presented at Microscopy and Microanalysis 2005 in Honolulu, Hawaii, USA, July 31--August 4,
Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.
Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – 5, 2010.
Journal Article Electron Tomographic Comparison of Frozen-Hydrated and Freeze-Substituted Sections High-Pressure Frozen Rat-Liver Tissue Get access C-E Hsieh, Hsieh Resource for Visualization Biological Complexity, Wadsworth Center, Albany, NY 12201 Search other works by this author on: Oxford Academic Google Scholar M Marko, Marko A Leith, Leith J Frank, Frank 12201Howard Hughes Medical Institute, Empire State Plaza, CA Mannella Microscopy Microanalysis, Volume 9, Issue S02, 1 August 2003,...
Abstract The multi-layered architecture of alphaviruses is built up with the registration nucleocapsid (NC) proteins and envelope spike across a lipid bilayer. At surface NC, capsid are organized as 42 capsomers such that three tails from each complex bind capsomers. Alphaviruses have been extensively studied using cryo-EM X-ray crystallography at near-atomic resolution (see review by Sedzik et al.). This work involves averaging images many isolated virions, makes use symmetry particle....
Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – 8, 2013.
Abstract Electron microscopic tomography is proving to be a valuable tool for investigating the 3D structure and organization of cellular organelles. Important progress being made in application technique frozen-hydrated material, but it likely that success with thick specimens will limited by low contrast beam sensitivity naked biological material. Thus, optimizing procedures fixing, embedding, staining, selectively labelling cells electron microscopy remains priority. Tomography chemically...
Extract Extended abstract of a paper presented at Microscopy and Microanalysis 2007 in Ft. Lauderdale, Florida, USA, August 5 – 9,
Extended abstract of a paper presented at Microscopy and Microanalysis 2007 in Ft. Lauderdale, Florida, USA, August 5 – 9,
Extract HTML view is not available for this content. However, as you have access to content, a full PDF via the ‘Save PDF’ action button. Extended abstract of paper presented at Microscopy and Microanalysis 2005 in Honolulu, Hawaii, USA, July 31--August 4,
Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005