A5086761669- Advanced Electron Microscopy Techniques and Applications
- Neuroscience and Neural Engineering
- Mitochondrial Function and Pathology
- Electrochemical Analysis and Applications
- Advanced Fluorescence Microscopy Techniques
- Hydrogels: synthesis, properties, applications
- Cell Image Analysis Techniques
- Nuclear Structure and Function
- Neural dynamics and brain function
- Metabolomics and Mass Spectrometry Studies
- Pickering emulsions and particle stabilization
- Heat shock proteins research
- Genetics and Neurodevelopmental Disorders
- Ocular Surface and Contact Lens
- 3D Printing in Biomedical Research
- Plant and Fungal Interactions Research
- Advanced Drug Delivery Systems
- Advancements in Transdermal Drug Delivery
- Proteins in Food Systems
- Plant Virus Research Studies
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Metabolism and Genetic Disorders
- Cellular Mechanics and Interactions
- Epigenetics and DNA Methylation
Massachusetts Institute of Technology
2020-2025
Harvard University
2016-2018
Center for Pain and the Brain
2016-2018
Wadsworth Center
2010-2013
New York State Department of Health
2013
Abstract Nuclear pore complexes (NPCs) create large conduits for cargo transport between the nucleus and cytoplasm across nuclear envelope (NE) 1–3 . These multi-megadalton structures are composed of about thirty different nucleoporins that distributed in three main substructures (the inner, cytoplasmic nucleoplasmic rings) around central channel 4–6 Here we use cryo-electron tomography on DLD-1 cells were prepared using cryo-focused-ion-beam milling to generate a structural model human NPC...
Resolving patterns of synaptic connectivity in neural circuits currently requires serial section electron microscopy. However, complete circuit reconstruction is prohibitively slow and may not be necessary for many purposes such as comparing neuronal structure among multiple animals. Here, we present an alternative strategy, targeted specific types. We used viral vectors to deliver peroxidase derivatives, which catalyze production electron-dense tracer, genetically identify neurons,...
Abstract Deep brain stimulation with implanted electrodes has transformed neuroscience studies and treatment of neurological psychiatric conditions. Discovering less invasive alternatives to deep could expand its clinical research applications. Nanomaterial-mediated transduction magnetic fields into electric potentials been explored as a means for remote neuromodulation. Here we synthesize magnetoelectric nanodiscs (MENDs) core–double-shell Fe 3 O 4 –CoFe 2 –BaTiO architecture (250 nm...
Lithium–metal batteries employing solid electrolytes (ceramics or polymers) could surpass the energy and power densities of current state-of-the-art lithium-ion batteries. Unfortunately, ceramic electrolyte/electrode interfaces suffer from poor interfacial contact, polymer show insufficient ionic conductivities for practical uses. Composite electrolytes, comprised mixtures mitigate these challenges by combining high conductivity with good contact. However, it is imperative to understand...
Patchy particle interactions are predicted to facilitate the controlled self-assembly and arrest of particles into phase-stable morphologically tunable "equilibrium" gels, which avoids arrested phase separation subsequent aging that is typically observed in traditional gels with isotropic interactions. Despite these promising traits patchy interactions, such equilibrium have yet be realized laboratory due experimental limitations associated synthesizing high yield. Here, we introduce a...
Abstract Lesion verification and quantification is traditionally done via histological examination of sectioned brains, a time-consuming process that relies heavily on manual estimation. Such methods are particularly problematic in posterior cortical regions ( e . g visual cortex), where sectioning leads to significant damage distortion tissue. Even more challenging the post hoc localization micro-electrodes, which same techniques, suffers from similar drawbacks requires even higher...
The interaction between excitons and photons underlies a range of emergent technologies, such as directional light emission, molecular lasers, photonic circuits, polaritonic devices. Two the key parameters that impact exciton–photon coupling are binding energy relative orientations exciton dipole photon field. Tightly bound typically found in crystals, where nevertheless angular relationship with fields is difficult to control. Here, we demonstrate dipoles fields, anchored by metal–ligand...
Abstract This study provides evidence for the Golgi‐like activity of multilayered interlaced network ( MIN ) and new ultrastructural observations in sporoplasm Anncaliia algerae , a microsporidium that infects both insects humans. The is attached to end polar tubule upon extrusion from germinating spore. It surrounds sporoplasm, immediately below its plasma membrane, most likely maintains integrity as it pulled through everting tube. Furthermore, appears deposit dense contents on surface...
SUMMARY Three-dimensional (3D) visualization of vitrified cells can uncover structures subcellular complexes without chemical fixation or staining. Here, we present a pipeline integrating three imaging modalities to visualize the same specimen at cryogenic temperature different scales: cryo-fluorescence confocal microscopy, volume cryo-focused ion beam scanning electron and transmission cryo-electron tomography. Our proof-of-concept benchmark revealed 3D distribution organelles in whole...
Lesion and electrode location verification are traditionally done via histological examination of stained brain slices, a time-consuming procedure that requires manual estimation. Here, we describe simple, straightforward method for quantifying lesions locating electrodes in the is less laborious yields more detailed results. Whole brains with osmium tetroxide, embedded resin, imaged micro-CT scanner. The scans result 3D digital volumes resolutions virtual section thicknesses dependent on...
Abstract Studies on the function of Methyl CpG binding protein 2 (MECP2) and consequence MECP2 deficiency duplication have largely focused neurons. The in human glia, along with comprehensive understanding glial neurodevelopmental disorders, is much less understood. Using female male embryonic stem cell (hESC) lines to model loss-of-function (LOF) Rett Syndrome (RTT) developing brain, we investigated molecular underpinnings astrocyte (AST) development dysfunction, mechanisms by which AST...
ABSTRACT All cells are subject to geometric constraints, such as surface area-to-volume (SA/V) ratio, that impact cell functions and force biological adaptations. Like the SA/V ratio of a sphere, it is generally assumed decreases size increases. Here, we investigate this in near-spherical mammalian using single-cell measurements mass proteins, well imaging plasma membrane morphology. We find remains surprisingly constant grow larger. This observation largely independent cycle amount growth....
Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – 5, 2010.
ARTICLES DISCUSSED: Truong, C. D. et al. Sample preparation using a lipid monolayer method for electron crystallographic studies. Journal of Visualized Experiments. (177), e63015 (2021). Johnson, M. C., Grant, A. J., Schmidt-Krey, I. The peel-blot technique: A cryo-EM sample to separate single layers from multi-layered or concentrated biological samples. (184), e64099 (2022). Chang, Y.-C., Chen, C.-Y., Tsai, M.-D. Preparation high-temperature grids cryo-EM. (173), e62772 Kang, M.-H., Lee,...