A5089321571- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Analytical Chemistry and Sensors
- Advanced Sensor and Energy Harvesting Materials
- Planarian Biology and Electrostimulation
- 3D Printing in Biomedical Research
- Advanced Memory and Neural Computing
- Conducting polymers and applications
- Electrochemical sensors and biosensors
- Gene Regulatory Network Analysis
- Pluripotent Stem Cells Research
- Neural dynamics and brain function
- Cephalopods and Marine Biology
- Cell Image Analysis Techniques
- Electrochemical Analysis and Applications
- Fuel Cells and Related Materials
- Plant and Biological Electrophysiology Studies
- Dielectric materials and actuators
- Wound Healing and Treatments
- bioluminescence and chemiluminescence research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced NMR Techniques and Applications
- Solid-state spectroscopy and crystallography
- Proteoglycans and glycosaminoglycans research
- Microfluidic and Capillary Electrophoresis Applications
University of California, Santa Cruz
2017-2024
Tufts University
2020
Continuous glucose monitoring from sweat and tears can improve the quality of life diabetic patients provide data for more accurate diagnosis treatment. Current continuous sensors use enzymes with a one-to-two week lifespan, which forces periodic replacement. Metal oxide are an alternative to enzymatic longer lifetime. However, metal do not operate in because they function at high pH (pH > 10), neutral = 7). Here, we introduce non-enzymatic sensor that functions fluids by electronically...
Precise modulation of brain activity is fundamental for the proper establishment and maturation cerebral cortex. To this end, cortical organoids are promising tools to study circuit formation underpinnings neurodevelopmental disease. However, ability manipulate neuronal with high temporal resolution in remains limited. overcome challenge, we introduce a bioelectronic approach control organoid selective delivery ions neurotransmitters. Using approach, sequentially increased decreased potassium (K
Abstract Organ-on-a-chip systems combine microfluidics, cell biology, and tissue engineering to culture 3D organ-specific in vitro models that recapitulate the biology physiology of their vivo counterparts. Here, we have developed a multiplex platform automates individual organoids isolated microenvironments at user-defined media flow rates. Programmable workflows allow use multiple reagent reservoirs may be applied direct differentiation, study temporal variables, grow cultures long term....
Abstract The ability to deliver drugs with precise dosages at specific time points can significantly improve disease treatment while reducing side effects. Drug encapsulation for gradual delivery has opened the doors a superior regimen. To expand on this ability, programming bioelectronic devices small molecules enables ad‐hoc personalized therapeutic profiles that are more complex than release. Here, wearable device an integrated electrophoretic ion pump affords on‐demand drug dose control...
Proton conductivity is important in many natural phenomena including oxidative phosphorylation mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, proton actuated bioluminescence dinoflagellate. In all of these phenomena, conduction protons occurs along chains hydrogen bonds between water hydrophilic residues. These are also present hydrated biopolymers macromolecule collagen, keratin, chitosan, various proteins such as reflectin. All materials conductors....
From the simplest unicellular organisms to complex animals, feedback control based on sensing and actuation is a staple of self‐regulation in biological processes key life itself. Malfunctioning this loop can often lead disease or death. Bioelectronic devices that interface electronics with systems be used for have potential novel therapeutic applications. Due complexity challenge affecting their innate self‐regulation, closing between bioelectronics difficult achieve. Herein, bioelectronic...
Simultaneous longitudinal imaging across multiple conditions and replicates has been crucial for scientific studies aiming to understand biological processes disease. Yet, systems capable of accomplishing these tasks are economically unattainable most academic teaching laboratories around the world. Here, we propose Picroscope, which is first low-cost system simultaneous made primarily using off-the-shelf 3D-printed materials. The Picroscope compatible with standard 24-well cell culture...
The development of wearable bioelectronic systems is a promising approach for optimal delivery therapeutic treatments. These can provide continuous ions, charged biomolecules, and an electric field various medical applications. However, rapid prototyping controlled specific treatments with scalable fabrication process challenging. We present system comprised polydimethylsiloxane (PDMS) device cast in customizable 3D printed molds circuit board (PCB), which employs commercially available...
Exactly 50 years ago, the ground-breaking discovery of dibenzo[18]crown-6 (DB18C6) by Charles Pedersen led to use DB18C6 as a receptor in supramolecular chemistry and host host–guest chemistry. We have demonstrated proton conductivity Tröger's base-linked polymers through hydrogen-bonded networks formed from adsorbed water molecules on oxygen atoms under humid conditions. polymers—poly(TBL-DB18C6)-t poly(TBL-DB18C6)-c—synthesized situ alkylation cyclization either trans- or...
Bioelectronic devices have shown their unprecedented potential in a wide range of biomedical applications due to vast functionality. To fully unleash potential, bioelectronic should be able precisely respond real-time changes the environment drive biological systems' response towards desired goals. However, controlling with is challenging presence uncertainties, stochasticity, unmodeled dynamics, and complex nonlinearities. Here, we demonstrate promise leveraging tools synthetic biology an...
A potentiostat is an essential piece of analytical equipment for studying electrochemical devices and reactions. As the design evolve, applications systems with multiple working electrodes have become more common. These drive a need low-cost multi-channel systems. We developed portable, scalable system modular that can support 8 to 64 channels at cost as low $8 per channel. This replace functionality commercial potentiostats which upwards $10k certain applications. Each channel in has...
Translation between ionic currents and measurable electronic signals is essential for the integration of natural systems artificial bioelectronic devices. Chloride ions (Cl−) play a pivotal role in bioelectricity, they are involved several brain pathologies, including epilepsy disorders autistic spectra, as well cancer birth defects. As such, controlling [Cl−] solution can actively influence biochemical processes be used therapies. Here, we demonstrate device that uses Ag/AgCl contacts to...
Abstract Bioelectronic devices that modulate pH can affect critical biological processes including enzymatic activity, oxidative phosphorylation, and neuronal excitability. A major challenge in controlling is the high buffering capacity of many media. To overcome this challenge, need to be able store deliver a large number protons on demand. Here, bioelectronic modulator controls using palladium nanoparticles contacts with surface area as proton storage medium developed. Reversible...
Bioelectronic devices that interface electronics with biological systems can actuate and control processes. The potassium ion plays a vital role in cell membrane physiology, maintaining the potential (Vmem) generating action potentials. In this work, we present two bioelectronic pumps use an electronic signal to modulate concentration solution. first pump is designed integrate directly six-well culture plates for optimal ease of integration vitro culture, second on-chip provides high spatial...
Abstract A balanced concentration of ions is essential for biological processes to occur. For example, [H + ] gradients power adenosine triphosphate synthesis, dynamic changes in [K and [Na create action potentials neuronal communication, [Cl − contributes maintaining appropriate cell membrane voltage. Sensing ionic thus important monitoring regulating many processes. This work demonstrates an ion‐selective microelectrode array that simultaneously independently senses ], electrolyte...
Bioelectronics integrates electronic devices and biological systems with the ability to monitor control processes. From homeostasis sensorimotor reflexes, closed-loop feedback is a staple of most fundamental life itself. Apart from few examples in bioelectronic medicine, processes using bioelectronics not as widespread nature. We note that adoption has been slow because traditional methods are difficult apply complex dynamics their sensitivity environmental changes. Here, we postulate...
Wound healing is a complex physiological process that requires precise control and modulation of many parameters. Therapeutic ion biomolecule delivery has the capability to regulate wound beneficially. However, achieving controlled through compact device with ability deliver multiple therapeutic species can be challenge. Bioelectronic devices have emerged as promising approach for delivery. Here, we present pro-reparative bioelectronic designed ions biomolecules applications. The...
Bioelectronic devices can provide an interface for feedback control of biological processes in real-time based on sensor information tracking response. The main challenges are guaranteeing system convergence the presence saturating inputs into bioelectronic device and complexities from indirect systems. In this paper, we first derive a saturated-based robust sliding mode design partially unknown nonlinear with disturbance. Next, develop data informed model silico simulations. Our controller...
ABSTRACT Precise modulation of brain activity is fundamental for the proper establishment and maturation cerebral cortex. To this end, cortical organoids are promising tools to study circuit formation underpinnings neurodevelopmental disease. However, ability manipulate neuronal with high temporal resolution in remains limited. overcome challenge, we introduce a bioelectronic approach control organoid selective delivery ions neurotransmitters. Using approach, sequentially increased decreased...
Bioelectronics In article number 2000140, Michael Levin, Marcella Gomez, Marco Rolandi, and co-workers present bioelectronics coupled with machine learning for intelligent control of cellular function. The authors set maintain the membrane voltage human pluripotent stem cells H+-conducting bioelectronic devices. H+-devices are actuated in a closed feedback loop respond to required adjustments cell as measured fluorescent reporters.
ABSTRACT Organ-on-a-chip systems combine microfluidics, cell biology, and tissue engineering to culture 3D organ-specific in vitro models that recapitulate the biology physiology of their vivo counterparts. Here, we have developed a multiplex platform automates individual organoids isolated microenvironments at user-defined media flow rates. Programmable workflows allow use multiple reagent reservoirs may be applied direct differentiation, study temporal variables, grow cultures long term....
In article number 1800935, Marco Rolandi and co-workers present a bioelectronic proton pump based on palladium hydride that changes pH in physiological conditions.