A5074008006- Cervical Cancer and HPV Research
- AI in cancer detection
- Oral Health Pathology and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Head and Neck Cancer Studies
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Salivary Gland Tumors Diagnosis and Treatment
- Gene expression and cancer classification
- Oral and Maxillofacial Pathology
- Cancer Cells and Metastasis
- Cancer Diagnosis and Treatment
- Genital Health and Disease
- Lung Cancer Treatments and Mutations
- Optical Coherence Tomography Applications
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Lung Cancer Diagnosis and Treatment
- Optical Imaging and Spectroscopy Techniques
- Genetic factors in colorectal cancer
- Thyroid Cancer Diagnosis and Treatment
- Cell Image Analysis Techniques
- Esophageal Cancer Research and Treatment
- Cancer Research and Treatments
- Prostate Cancer Treatment and Research
BC Cancer Agency
2010-2024
University of British Columbia
2012-2024
Occupational Cancer Research Centre
2012-2023
Breast Cancer Research Foundation
2019-2023
Spinal Cord Injury BC
2019-2021
Cancer Research Center
2008-2021
Workers Compensation Board of Alberta
2018
Memorial Sloan Kettering Cancer Center
2018
The University of Texas MD Anderson Cancer Center
2018
Allegheny General Hospital
2018
The <i>Journal of Biomedical Optics</i> (JBO) is a Gold Open Access journal that publishes peer-reviewed papers on the use novel optical systems and techniques for improved health care biomedical research.
Background: Results from preclinical studies have suggested that the organosulfur compound anethole dithiolethione (ADT) may be an effective chemopreventive agent for lung cancer. We conducted a phase IIb study to determine effects of ADT in smokers with bronchial dysplasia. Methods: One hundred twelve current and former smoking history at least 30 pack-years one site dysplasia identified by autofluorescence bronchoscopy-directed biopsy were randomly assigned receive placebo or 25 mg orally...
Abstract Oral cancer is one of the most commonly diagnosed cancers worldwide. Disease often at later stages, which associated with a poor 5‐year survival rate and high local recurrence. Micro RNA s (mi s), group small, noncoding s, can be isolated from blood serum samples have demonstrated utility as biomarkers in multiple types. The aim this study was to examine expression profiles circulating mi patients high‐risk oral lesions ( HRL s; or carcinoma situ) explore their potential biomarkers....
This paper addresses the problem of quantifying biomarkers in multi-stained tissues based on color and spatial information microscopy images tissue. A deep learning-based method that can automatically localize quantify regions expressing biomarker(s) any selected area a whole slide image is proposed. The learning network, which we refer to as Whole Image (WI)-Net, fully convolutional network whose input true RGB tissue output map showing locations each biomarker. WI-Net relies different...
Diagnosis and classification of oral epithelial dysplasia (OED) is critical to identifying prognosticating patients at risk squamous cell carcinoma (SCC). However, conventional 3-tiered 2-tiered grading systems suffer from poor inter-pathologist agreement, SCC may arise all grades OED. This study evaluated pathologist agreement in OED as p53 wildtype, abnormal, HPV-associated based on recent evidence demonstrating the utility p53/p16 immunohistochemistry (IHC) this setting increased abnormal...
The finite-difference time-domain (FDTD) method provides a flexible approach to studying the scattering that arises from arbitrarily inhomogeneous structures. We implemented three-dimensional FDTD program code model light biological cells. perfectly matched layer (PML) boundary condition has been used terminate computational grid. investigated differences in angle-dependent properties of normal and dysplastic cervical Specifically, patterns phase functions have computed for cells at three...
<h3>Background</h3> The tumor microenvironment (TME) is a complex mixture of epithelium, stroma and immune cells, the component TME highly prognostic for progression patient outcome. In lung cancer, anti-PD-1 therapy significantly improves survival through activation T cell cytotoxicity against cells. Direct contact between CD8+ cells target necessary activity, indicating that spatial organization within reflects critical process in anti-tumor immunity. Current immunohistochemistry (IHC)...
Confocal microscopy can provide real-time, 2-D and 3-D images of the cellular morphology tissue architecture features that pathologists use to detect precancerous lesions without need for removal, sectioning, staining. The utility confocal image stacks epithelial detecting dysplasia has not yet been explored. We aim extract morphometry information from reflectance fresh, unstained cervical biopsies compare their potential dysplasia. Nine are obtained eight patients; acquired pre- postacetic...
Abstract The importance of early diagnosis in improving mortality and morbidity rates oral squamous cell carcinoma (SCC) has long been recognized. However, a major challenge for is our limited ability to differentiate premalignant lesions (OPL) at high risk progressing into invasive SCC from those low risk. We investigated the potential quantitative tissue phenotype (QTP), measured by high-resolution image analysis, identify severe dysplasia/carcinoma situ (CIS; known have an increased...
<h3>Objective</h3> Long noncoding RNAs (lncRNAs) are a unique class of messenger RNA–like transcripts at least 200 nucleotides in length with no significant protein-coding capacity. Aberrant lncRNA expression is emerging as major component the cancer transcriptome. Here, we sought to determine if differential feature human cervical intraepithelial neoplasia (CIN) <h3>Methods</h3> Sequence data were derived from 16 long serial analyses gene (L-SAGE) libraries constructed specimens...
Current diagnostic capabilities and limitations of fluorescence endomicroscopy in the cervix are assessed by qualitative quantitative image analysis. Four cervical tissue types investigated: normal columnar epithelium, precancerous squamous stromal tissue. This study focuses on perceived variability within subtle differences between four groups context endomicroscopic vivo pathology. Conclusions drawn general ability to distinguish diagnose types, need for imaging depth control enhance...
Abstract Background Background: As part of a project to evaluate emerging optical technologies for cervical neoplasia, our group is performing quantitative histopathological analyses biopsy specimens from 1,190 patients. Objectives in the interim analysis are (a) quantitatively assessing progression neoplastic process intraepithelial neoplasia (CIN)/squamous lesions (SIL), (b) detecting malignancy‐associated changes (MACs), and (c) phenotypically measuring human papillomavirus (HPV) detected...
Because 80% of cervical cancers arise in low-resource settings, many inexpensive strategies are being tested. In that spirit, the authors testing large-scale genomic or DNA ploidy measurements as an and semiautomated strategy.Patients entered either a screening diagnostic study several optical technologies: quantitative cytology, histopathology, fluorescence reflectance spectroscopy using point probe, multispectral digital colposcope, combination two. We calculated sensitivities,...
Testing emerging technologies involves the evaluation of biologic plausibility, technical efficacy, clinical effectiveness, patient satisfaction, and cost-effectiveness. The objective this study was to select an effective classification algorithm for optical spectroscopy as adjunct colposcopy obtain preliminary estimates its accuracy detection CIN 2 or worse. We recruited 1,000 patients from screening prevention clinics 850 at two comprehensive cancer centers a community hospital. Optical...
We use an extensive set of quantitative histopathology data to construct realistic three-dimensional models normal and dysplastic cervical cell nuclei at different epithelial depths. then employ the finite-difference time-domain method numerically simulate light scattering response these representative as a function polar azimuthal angles. The results indicate that intensity shape metrics computed from two-dimensional patterns can be used distinguish between diagnostic categories. Our...
Cervical cancer remains a major health problem, especially in developing countries. Colposcopic examination is used to detect high-grade lesions patients with history of abnormal pap smears. New technologies are needed improve the sensitivity and specificity this technique. We propose test potential fluorescence confocal microscopy identify lesions. examined quantification ex vivo differentiate among normal cervical tissue, low-grade Intraepithelial Neoplasia (CIN), CIN. sought (1) quantify...
Objectives : As part a Program Project to evaluate emerging optical technologies for cervical neoplasia, our group is performing quantitative histopathological analysis of biopsies from 1800 patients. Several methodological issues have arisen with respect this analysis: (1) Finding the most efficient way compensate staining intensity variation out losing diagnostic information; (2) Assessing inter‐ and intra‐observer variability semi‐interactive data collection; (3) use non‐overlapping cells...
The highest rates of cervical cancer are found in developing countries. Frontline monitoring has reduced these developed countries and present day screening programs primarily identify precancerous lesions termed intraepithelial neoplasias (CIN). CIN described as mild dysplasia (CIN I) likely to spontaneously regress while III (severe dysplasia) progress if untreated. Thoughtful consideration gene expression changes paralleling the progressive pre invasive neoplastic development will yield...
Accurate cervical intra-epithelial neoplasia (CIN) lesion grading is needed for effective patient management. We applied computer-assisted scanning and analytic approaches to immuno-stained CIN sections more accurately delineate disease states decipher cell proliferation impacts from HPV smoking within individual epithelial layers. A cohort undergoing screening was identified (n = 196) biopsies of varying grades with intact basement membranes layers were obtained 261). Specimens sectioned,...