A5017360241- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Cytomegalovirus and herpesvirus research
- Rabies epidemiology and control
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Malaria Research and Control
- Congenital Anomalies and Fetal Surgery
- Parasitic infections in humans and animals
- Travel-related health issues
- Heme Oxygenase-1 and Carbon Monoxide
- Vector-borne infectious diseases
- interferon and immune responses
- Diagnosis and treatment of tuberculosis
- Fungal Biology and Applications
- Peptidase Inhibition and Analysis
- Ocular Diseases and Behçet’s Syndrome
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Vector-Borne Animal Diseases
- Clinical Laboratory Practices and Quality Control
- Clinical Reasoning and Diagnostic Skills
- Genomics and Phylogenetic Studies
- Hepatitis Viruses Studies and Epidemiology
Université de Strasbourg
2009-2023
Hôpitaux Universitaires de Strasbourg
2007-2023
Institut d’Epidémiologie Neurologique et de Neurologie Tropicale
2002-2019
Centre Hospitalier Universitaire de Montpellier
2010-2015
Architecture et Réactivité de l'arN
2013
Institut de Biologie Moléculaire et Cellulaire
2013
Inserm
1999-2004
Biologie et Génétique des Interactions Plante-Parasite
2002
Centre National de la Recherche Scientifique
1999
To study the influence of Toxoplasma gondii genotypes on severity human congenital toxoplasmosis (asymptomatic, benign, or severe infection newborn fetal death), 8 microsatellite markers were used to analyze 86 T. isolates collected from patients with toxoplasmosis. Seventy-four different detected, some identical originating probably same source contamination. The 3 less polymorphic associated 6 isoenzymatic allowed a classification into classical types and detected atypical genotypes....
We report the genotyping analysis of Toxoplasma gondii isolates in samples collected from 88 immunocompromised patients, along with clinical and epidemiological data. Most these were France during current decade by Biological Resource Center. Lack specific anti-Toxoplasma treatment, pulmonary toxoplasmosis, involvement multiple organs 3 main risk factors associated death for this patient group. Genotyping results 6 microsatellite markers showed that type II predominant among patients who...
ABSTRACT Toxoplasmosis is a life-threatening infection in immunocompromised patients (ICPs). The definitive diagnosis relies on parasite DNA detection, but little known about the incidence and burden of disease HIV-negative patients. A 3-year retrospective study was conducted 15 reference laboratories from network French National Reference Center for Toxoplasmosis, order to record frequency Toxoplasma gondii detection ICPs review molecular methods used prevention measures implemented...
ABSTRACT Ocular toxoplasmosis is the major cause of posterior uvetis in European populations. The clinical diagnosis toxoplasmic chorioretinitis based upon ophthalmoscopic findings, which are often but not always typical. Laboratory testing therefore important to confirm etiology disease. In present 2-year prospective study, relative diagnostic sensitivities three analytical techniques (enzyme-linked immunosorbent assay [ELISA], immunoblotting, and PCR) were compared by using a group...
Although screening for maternal toxoplasmic seroconversion during pregnancy is based on immunodiagnostic assays, the diagnosis of clinically relevant toxoplasmosis greatly relies upon molecular methods. A problem that this subject to variation performances, mainly due a large diversity PCR methods and primers lack standardization. The present multicentric prospective study, involving eight laboratories proficient in prenatal toxoplasmosis, was first step toward harmonization among university...
Plasmodium vivax is considered to be absent from western Africa, where the prevalence of Duffy-negative red blood cell phenotype proves high. Several studies have, however, detected P. infection cases in this part raising question what actual local populations.The presence was investigated a large population healthy donors Benin using microscopy, serology and molecular detection. The seroprevalence measured with species-specific ELISA two recombinant proteins, namely rPvMSP1 rPvCSP1....
Malaria Is A Life-Threatening Pathology In Africa. Plasmodium Falciparum And Vivax Attract The Most Focus Because Of Their High Prevalence Mortality. Knowledge About Cryptic Pathogens Ovale Malariae Limited. Thanks To Recombinant Tools, Seroprevalence Was Measured For First Time, As Well Mixed Infections Malaria-Asymptomatic Population Benin, Malaria-Endemic Country.A Panel 1,235 Blood Donations Collected Over Ten Months Benin Used Validation Tools. P. Falciparum, Malariae, MSP1, AMA1 Were...
ABSTRACT We evaluated the effect of vaccination with SAG1 protein Toxoplasma gondii against congenital toxoplasmosis in mice different genetic backgrounds. In BALB/c ( H-2 d ), reduced number infected fetuses by 50% and was associated a mixed type 1 2 immunity. CBA/J k increased predominant response. Our results indicate that is controlled background mouse.
Protective immunity in mice infected with Toxoplasma gondii is mainly mediated by NK cells, CD4 and CD8 T type 1 cytokines, such as gamma interferon (IFN-gamma). To clarify the roles of cells IFN-gamma protection against primary congenital toxoplasmosis, we used recombination activating gene 2 knockout (RAG-2(-/-)) mice, which lack B lymphocytes, comparison wild-type BALB/c model. RAG-2(-/-) had a significantly lower risk fetal toxoplasmosis than (25 versus 63.9%; P = 0.003). This was...
ABSTRACT Over the past few years, a number of new nucleic acid extraction methods and platforms using chemistry combined with magnetic or silica particles have been developed, in combination instruments to facilitate procedure. The objective present study was investigate suitability these automated for isolation Toxoplasma gondii DNA from amniotic fluid (AF). Therefore, three procedures were compared two commercialized manual methods. MagNA Pure Compact (Roche), BioRobot EZ1 (Qiagen),...
ABSTRACT Three PCR targets (18S ribosomal DNA, B1, and AF146527) mouse inoculation were compared for 83 samples in the context of congenital toxoplasmosis. These four techniques are not statistically different terms sensitivity specificity. However, further analysis highlighted problems sometimes encountered with diagnosis
A 72-year-old man consulted in November 2012 for abdominal pain the right upper quadrant. The patient had a history of suspected hepatic amebiasis treated Senegal 1985 and has not traveled to endemic areas since 1990. Abdominal CT scan revealed liver abscess. At first, no parasitological tests were performed was with broad-spectrum antibiotics. Only after failure this therapy, serology PCR abscess puncture established diagnosis amebiasis. metronidazole tiliquinol-tilbroquinol. Amebic is most...
To describe the lesions detected by prenatal ultrasound examination in congenital toxoplasmosis (CT).We retrospectively analyzed all cases of fetal infection with Toxoplasma gondii anomalies described medicine experts 2009 to 2019 30 French centers.Eighty-eight CT were included. Forty-five (51.1%) had one or more cerebral signs only, 35 (39.8%) plus extracerebral and 8 (9.1%) only. The main intracranial hyperechogenic nodular foci (n = 60) which 20 isolated, ventriculomegalies 44) generally...
ABSTRACT The report presents two cases where diagnosis of alveolar echinococcosis was confirmed by Echinococcus multilocularis and granulosus PCR. extrahepatic osseous involvement the absence initial hepatic are unusual in both cases. Due to limitations serological interpretation, PCR useful diagnose atypical echinococcosis.
The detection of Toxoplasma gondii in amniotic fluid is an essential tool for the prenatal diagnosis congenital toxoplasmosis and currently essentially based on use PCR. Although some consensus emerging, this molecular suffers from a lack standardization extreme diversity laboratory-developed methods. Commercial kits T. by PCR were recently developed offer certain advantages; however, they must be assessed comparison with optimized reference assays. present multicentric study aimed to...
ABSTRACT We evaluated the molecular diagnosis of congenital toxoplasmosis (CT) on neonatal amniotic fluid samples from 488 mother-child pairs. Maternal infection during pregnancy was diagnosed and dated or could not be ruled out. Forty-six cases CT were defined according to European Research Network classification system case definitions. Neonatal testing had an overall sensitivity 54% (95% confidence interval [95% CI], 39 69%) a specificity 100% CI, 99 100%). Its 33% 13 59%) when antenatal...
In the BALB/c mouse model, primary infection with Toxoplasma gondii during second third of gestation leads to a high percentage infected foetuses. However, immunity induced by contracted before pregnancy prevents parasites from crossing placenta and completely protects foetuses, as well pregnant women. order clarify roles CD4+, CD8+ T lymphocytes IFN-gamma in this protection, mice were treated depleting monoclonal antibodies against CD4, CD8, IFN-gamma, or control antibody. Only foetuses...
We examined 785 placentas, including 51 from documented cases of congenital toxoplasmosis. Toxoplasma was detected in 16 1 which toxoplasmosis ruled out. Placental screening had poor sensitivity (25%) but good specificity (99%), positive predictive value (93%), and negative (95%).