A5014689092- Protein Structure and Dynamics
- Spectroscopy and Quantum Chemical Studies
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Infective Endocarditis Diagnosis and Management
- Computational Drug Discovery Methods
- Cardiac Valve Diseases and Treatments
- Cardiac, Anesthesia and Surgical Outcomes
- Acute Kidney Injury Research
- Aortic Disease and Treatment Approaches
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Chronic Kidney Disease and Diabetes
- Phagocytosis and Immune Regulation
- X-ray Diffraction in Crystallography
- Hormonal Regulation and Hypertension
- Crystallization and Solubility Studies
- Molecular spectroscopy and chirality
- Peripheral Artery Disease Management
- Renin-Angiotensin System Studies
- Cellular transport and secretion
- Cardiac Structural Anomalies and Repair
- Machine Learning in Bioinformatics
- Eicosanoids and Hypertension Pharmacology
- Vascular Procedures and Complications
Dokkyo Medical University
2019-2024
Hospital for Sick Children
2005-2023
Sanyo-Onoda City University
2020-2021
Hitachi (Japan)
2010-2019
University of Miyazaki Hospital
2013-2019
Kyushu University
2004-2019
RIKEN
2012-2018
The Nakamura Hajime Eastern Institute
2012-2018
Nippon Soken (Japan)
2014-2016
Kōchi University
2009-2016
Autophagy is a cellular recycling process induced in response to many types of stress. However, little known the signaling pathways that regulate autophagy during acute kidney injury (AKI). Bcl-2/adenovirus E1B 19 kDa-interacting protein (BNIP)3 and sestrin-2 are target proteins hypoxia-inducible factor (HIF)-1α p53, respectively. The aim this study was investigate roles BNIP3 oxidative stress-induced AKI. We used rat ischemia-reperfusion cultured renal tubular (NRK-52E) cells as vivo vitro...
The electrochemical reduction of CO2 to CO by an ionic liquid EMIM-BF4 is one the most promising processes proposed so far with its high Faradaic efficiency and low overpotential. However, details reaction mechanism are still unknown due absence fundamental understandings. In this study, probable stable geometries were calculated quantum chemistry in combination exhaustive search. A possible pathway from catalyzed EMIM-BF4, including plausible intermediates corresponding transition states,...
Plasmalemmal phosphatidylinositol (PI) 4,5-bisphosphate (PI4,5P2) synthesized by PI 4-phosphate (PI4P) 5-kinase (PIP5K) is key to the polymerization of actin that drives chemotaxis and phagocytosis. We investigated means whereby PIP5K targeted membrane its fate during phagosome formation. Homology modeling revealed all isoforms feature a positively charged face. Together with substrate-binding loop, this polycationic surface proposed constitute coincidence detector targets PIP5Ks...
<h3>Background</h3> Bardet–Biedl syndrome is a pleiotropic disorder with 14 BBS genes identified. BBS1, BBS2, BBS4, BBS5, BBS7, BBS8, and BBS9 form complex called the BBSome, which believed to recruit Rab8<sup>GTP</sup> primary cilium promote ciliogenesis. The second group, chaperonin-like proteins BBS6, BBS10, BBS12, have been defined as vertebrate-specific branch of type II chaperonin superfamily. These may play role in regulation BBSome assembly. <h3>Methods results</h3> Using sequence...
The molecular dynamics simulation is reported. latest data on photosystem II structure, a thylakoid membrane model with the same lipid class distribution and fatty acid composition as native membrane, are used. results indicate that transfer of water, oxygen protons has different pathways. root mean square (rms)-fluctuation analysis trajectory revealed residues surrounding oxygen-evolving center (OEC) show small fluctuations most water molecules there large fluctuation proposed pathways for...
Background Heat shock protein beta-1 (HSPB1, also known as HSP27) is a small heat involved in many cellular processes and reportedly protects cells against oxidative stress. Autophagy from types of stress thought to play key role preventing acute kidney injury (AKI). However, little about the HSPB1 autophagy apoptosis pathogenesis AKI. Methods We used rat ischemia/reperfusion AKI model cultured renal tubular an vitro model. To elucidate regulation HSPB1, we evaluated promoter activity...
The enzyme urease is an essential colonizing factor of the notorious carcinogenic pathogen Helicobacter pylori (H. pylori), conferring acid resistance to bacterium. Recently, antibiotic resistant strains have emerged globally with little no alternative treatment available. In this study we propose novel inhibitors capable controlling infection by H. and other pathogenic bacteria. We employed hierarchal computational approaches screen new from commercial chemical databases followed in vitro...
Background/Aims Nephrotoxicity is a frequent and major limitation in cisplatin (CDDP)-based chemotherapy. 5-Aminolevulinic acid (ALA) widely distributed animal cells, it precursor of tetrapyrole compounds such as heme that fundamentally important aerobic energy metabolism. The aim this study to evaluate the protective role ALA CDDP-induced acute kidney injury (AKI). Method We used AKI rat model cultured renal tubular cells (NRK-52E). divided four groups rats: control, CDDP only, +...
A structure-based lead optimization procedure is an essential step to finding appropriate ligand molecules binding a target protein structure in order identify drug candidates. This takes known of protein-ligand complex as input, and structurally similar compounds with the query are designed consideration all possible combinations atomic species. task is, however, computationally hard since such combinatorial problems belong non-deterministic nonpolynomial-time (NP-hard) class. In this...
A set of conserved water positions making direct contacts with the alpha1 and alpha2 domains MHC class-I protein was identified by a cluster analysis in 12 high-resolution crystal structures proteins from different allele types species, comprising human, mouse rat. The revealed total 63 clusters, corresponding to molecules, whose are half or more analyzed structures. Analysis these clusters shows that most positions-those appearing largest fraction structures-were also accurately defined, as...
A consideration of the surrounding environment is necessary for a meaningful analysis reaction activity in large molecular systems. We propose an approach to perform unrestricted Hartree-Fock (UHF) calculations within framework fragment orbital (FMO) method (FMO-UHF) study systems with unpaired electrons. Prior energy one has optimize geometry, which requires accurate analytic gradient. derive FMO-UHF and its gradient implement them into GAMESS. The performance evaluated solvated organic...
In photosynthesis, calcium is crucial for oxygen evolution. the absence of Ca2+, Kok cycle has been proven to stop at S2 with Yz•. To explore reason, photosystem II (PSII) models (in total 32452 atoms) different metal ions (Ca2+, Sr2+, and K+) or without Ca2+ involved in evolution complex (OEC) have theoretically studied based on previous dynamic study PSII. It found that portion Mn1 d-orbital decreases highest occupied molecular orbitals Ca2+-depleted This feature unfavorable electron...