A5061529001- Carcinogens and Genotoxicity Assessment
- Toxic Organic Pollutants Impact
- Mycotoxins in Agriculture and Food
- Genomics, phytochemicals, and oxidative stress
- Thyroid Disorders and Treatments
- Pharmacogenetics and Drug Metabolism
- Animal Behavior and Welfare Studies
- Pharmacological Effects and Assays
- Plant Toxicity and Pharmacological Properties
- Biochemical effects in animals
- Milk Quality and Mastitis in Dairy Cows
- Drug Transport and Resistance Mechanisms
- Hormonal and reproductive studies
- Animal testing and alternatives
- Animal Nutrition and Physiology
- Wheat and Barley Genetics and Pathology
- Agriculture and Biological Studies
- Liver physiology and pathology
- Pharmaceutical and Antibiotic Environmental Impacts
- Probiotics and Fermented Foods
- PI3K/AKT/mTOR signaling in cancer
- Healthcare and Venom Research
- Animal Disease Management and Epidemiology
- Animal health and immunology
- Cassava research and cyanide
University of Turin
2016-2025
Bitron (Italy)
2015
Candiolo Cancer Institute
2005-2011
AstraZeneca (Italy)
2010
Ionizing radiation (IR) is effectively used in cancer therapy. However, subsets of patients, a few radioresistant cells survive and cause disease relapse with metastatic progression. The MET oncogene encodes the hepatocyte growth factor (HGF) receptor known to drive "invasive growth", regenerative prosurvival program unduly activated metastasis.Human tumor cell lines (MDA-MB-231, MDA-MB-435S, U251) were subjected therapeutic doses IR. mRNA, protein expression signal transduction compared...
Due to the climatic change, an increase in aflatoxin B1 (AFB1) maize contamination has been reported Europe. As alternative mineral binders, natural phytogenic compounds are increasingly used counteract negative effects of AFB1 farm animals. In cows, even low dietary concentrations may result milk excretion genotoxic carcinogen metabolite M1 (AFM1). this study, we tested ability turmeric powder (TP), extract from Curcuma longa (CL) rich curcumin and curcuminoids, reducing AFM1 mammary...
Aflatoxin B1 (AFB1) causes hepatotoxicity, immunotoxicity, and kidney damage, it is included in group I of human carcinogens. The European Commission has established maximum limits AFB1 feed, ranging from 5 to 20 μg/kg. Chicken moderately sensitive AFB1, which results reduced growth performance economic losses. Oxidative stress triggered by plays a crucial role damage the antioxidant activity Curcumin (CURC) could help preventing such adverse effect. Twenty-days-old broilers were treated for...
Sorghum plants naturally produce dhurrin, a cyanogenic glycoside that may be hydrolysed to cyanide, resulting in often-lethal toxicoses. Ruminants are particularly sensitive glycosides due the active role of rumen microbiota dhurrin hydrolysis. This work provides an overview poisoning outbreak occurred 5 farms Northwest Italy August 2022; total 66 cows died, and many others developed acute toxicosis after being fed on either cultivated (Sorghum bicolor) or wild halepense). Clinical signs...
Aflatoxin B1 (AFB1) can impair the growth of chickens and reduce quality eggs meat, resulting in significant economic losses. The inclusion mycotoxin detoxifying agents (MyDA) with binding properties diet is an efficient tool to their absorption rate gastrointestinal tract. Our aim was investigate ability two innovative MyDA (SeOX, a feed additive featuring tri-octahedral smectite mixed lignocellulose, CHS, di-octahedral functionalized organic non-toxic modifier) both reducing...
Oleander is a spontaneous shrub widely occurring in Mediterranean regions. Poisoning sporadically reported livestock, mainly due to the ingestion of leaves containing toxic cardiac glycosides (primarily oleandrin). In this study, 50 lactating Fleckvieh cows were affected after being offered diet dry oleander pruning wastes accidentally mixed with fodder. Clinical examination, electrocardiogram, and blood sampling conducted. Dead animals necropsied, heart, liver, kidney, spleen, intestine...
We examined whether inhibition of Src tyrosine kinase, a downstream effector the MET oncogene, can hinder malignant properties gastric tumors dependent on Met for growth and survival.Sensitivity to was determined in vitro by measuring clonogenic survival (anchorage-independent growth) vivo establishing xenograft models. Four "Met-addicted" carcinoma cell lines (GTL16, MKN45, HS746T, SNU5) three Met-independent (KATO III, AGS, NCI-N87) were treated with inhibitor saracatinib (AZD0530). In...
An ad hoc questionnaire was designed in order to investigate AMR knowledge amongst Italian dog owners, owner expectations concerning pharmacological treatment of canine AD, and client attitudes towards compliance with alternative strategies antimicrobial administration. A total 250 questionnaires were returned. Most respondents female, aged 36-70 workers. More than a half participants owned one mixed breed, Labrador retriever, golden dachshund, border collie being the most represented...
Dexamethasone is a potent synthetic corticosteroid widely employed as therapeutic agent in cattle. Besides this legal use, corticosteroids are also administered at low dosages growth-promoters either alone or combination with other steroids beta-agonists. For reason, appropriate control plans established to survey misuse, using liver urine biological matrices. Since few data available about the kinetics of dexamethasone excretion meat cattle, an experimental study was designed assess drug...
Canine acute enteropathies (AE) are common morbidities primarily managed with supportive therapy. However, in some cases, unnecessary courses of antibiotics empirically prescribed. Recent studies humans have hypothesized the use antioxidants as a possible alternative and/or support to antimicrobial drugs uncomplicated cases. Considering global need reduce antibiotic use, aim study was compare oxidative burden diarrhetic population that healthy dogs. Forty-five patients suffering from...
The most frequent adverse effects of AFB1 in chicken are low performance, the depression immune system, and a reduced quality both eggs meat, leading to economic losses. Since oxidative stress plays major role toxicity, natural products increasingly being used as an alternative mineral binders tackle toxicosis farm animals. In this study, vivo trial was performed by exposing broilers for 10 days at dietary concentrations approaching maximum limits set EU (0.02 mg/kg feed) presence or absence...
Sorghum plants naturally produce dhurrin, a cyanogenic glycoside which may be hydrolysed to cyanide resulting in often lethal toxicoses. Ruminants are particularly sensitive glycosides due the active role of rumen microbiota dhurrin hydrolysis. This work provides an overview poisoning outbreak occurred 5 farms from North-West Italy August 2022; total 66 cows died, and many others developed acute toxicosis after being fed on either cultivated- (Sorghum bicolor) or wild halepense). Clinical...
In cattle, phenobarbital (PB) upregulates target drug-metabolizing enzyme (DME) mRNA levels. However, few data about PB's post-transcriptional effects are actually available. This work provides the first, and an almost complete, characterization of PB-dependent changes in DME catalytic activities bovine liver using common probe substrates confirmatory immunoblotting investigations. As expected, PB increased total cytochrome P450 (CYP) content extent metyrapone binding; moreover, augmentation...
Virkel, G., Carletti, M., Cantiello, Della Donna, L., Gardini, Girolami, F., Nebbia, C. Characterization of xenobiotic metabolizing enzymes in bovine small intestinal mucosa. J. vet. Pharmacol. Therap.33, 295–303. The mucosa plays a capital role dictating the bioavailability large array orally ingested drugs and toxicants. activity expression several were measured subcellular fractions from duodenal male veal calves beef cattle displaying functional rumen but differing both age (about 8...
Prednisolone was one of the first glucocorticoids to be synthesised, but it is still widely applied cattle. Illegal uses prednisolone include its for masking a number diseases before animal sale and, at lower dosages extended periods time, improvement feed efficiency and carcass characteristics. Since occasional presence has been detected trace level in urine samples from untreated cattle, Italian Ministry Health introduced provisional limit 5 ng/mL avoid false non-compliances. However, this...
The ATP-binding cassette efflux transporter ABCG2 plays a key role in the mammary excretion of drugs and toxins humans animals. Aflatoxins (AF) are worldwide contaminants food feed commodities, while PCB 126 is dioxin-like which may contaminate milk dairy products. Both compounds known human carcinogens. interactions between AF bovine (bABCG2) as well effects on its activity have been investigated by means Hoechst H33342 transport assay MDCKII cells stably expressing bABCG2. AFB1 main...