Due to the climatic change, an increase in aflatoxin B1 (AFB1) maize contamination has been reported Europe. As alternative mineral binders, natural phytogenic compounds are increasingly used counteract negative effects of AFB1 farm animals. In cows, even low dietary concentrations may result milk excretion genotoxic carcinogen metabolite M1 (AFM1). this study, we tested ability turmeric powder (TP), extract from Curcuma longa (CL) rich curcumin and curcuminoids, reducing AFM1 mammary...

Poly- and perfluoroalkyl substances (PFAS) are frequently detected in the environment linked to adverse reproductive health outcomes humans. Although legacy PFAS have been phased out due their toxicity, alternative increasingly used despite fact that information on toxic effects traits is particularly scarce. Here, we exposed male guppies (Poecilia reticulata) for a short period (21 days) an environmentally realistic concentration (1 ppb) of PFOA, PFAS, its replacement compound, GenX, assess...

Due to their widespread diffusion, perfluoroalkyl substances (PFASs) have been frequently found in the environment and several animal species. It has demonstrated that they can easily reach also humans, mainly through diet. Being lactation a major route of elimination these contaminants, occurrence human milk is particular interest, especially considering it generally represents unique food source for newborns. Perfluorooctane sulfonate (PFOS) perfluorooctanoic acid (PFOA), two most...

Aflatoxin B1 (AFB1) toxicity in livestock and human beings is a major economic health concern. Natural polyphenolic substances with antioxidant properties have proven to be effective ameliorating AFB1-induced toxicity. Here we assessed the potential anti-AFB1 activity of curcumin (pure curcumin, C, from Curcuma longa, CL) bovine fetal hepatocyte-derived cell line (BFH12). First, measured viability cells exposed AFB1 presence or absence treatment. Then, explored all transcriptional changes...

Measuring purine metabolites in the serum and urine may help early diagnosis of urolith formation, correction allopurinol therapeutic dosages, evaluation diet-related alterations. To properly monitor these endogenous compounds assess their physiological concentration ranges biological fluids, highly specific accurate analytical approaches are required. Colorimetric assays generally used for this purpose, although cross-reactivity could lead to incorrect determinations. Given importance...

Background: Although antibiotic resistance is a well-known issue in veterinary medicine, studies proposing real-time therapeutic monitoring (TDM) are lacking. The objective of the present study was to develop simple and rapid protocol for antibiotics horses dogs. Methods: A reliable TDM should encompass guidelines definition plasma/serum collection time points, sample management by clinical staff, transportation laboratory, availability robust swift analytical technologies. Ampicillin...

Aflatoxins, and particularly aflatoxin B1 (AFB1), are toxic mycotoxins to humans farm animal species, resulting in acute chronic toxicities. At present, AFB1 is still considered a global concern with negative impacts on health, the economy, social life. In animals, exposure AFB1-contaminated feed may cause several untoward effects, liver damage being one of most devastating ones. present study, we assessed vitro transcriptional changes caused by bovine fetal hepatocyte-derived cell line...

Aflatoxin B1 (AFB1) is a natural feed and food contaminant classified as group I carcinogen for humans. In the dairy industry, AFB1 its derivative, AFM1, are of concern related economic losses their possible presence in milk products. Among toxic effects, can cause oxidative stress. Thus, dietary supplementation with antioxidants has been considered among strategies to mitigate toxicity. Here, protective role resveratrol (R) investigated foetal bovine hepatocyte cell line (BFH12) exposed...

Aflatoxin B1 (AFB1) is a food contaminant metabolized mostly in the liver and leading to hepatic damage. Livestock species are differently susceptible AFB1, but underlying mechanisms of toxicity have not yet been fully investigated, especially ruminants. Thus, aim present study was better characterize molecular mechanism by which AFB1 exerts hepatotoxicity cattle. The bovine fetal hepatocyte cell line (BFH12) exposed for 48 h three different concentrations (0.9 µM, 1.8 µM 3.6 µM)....

Aflatoxin B1 (AFB1) induces lipid peroxidation and mortality in bovine foetal hepatocyte-derived cells (BFH12), with underlying transcriptional perturbations associated mainly cancer, cellular damage, inflammation, bioactivation, detoxification pathways. In this cell line, curcumin resveratrol have proven to be effective mitigating AFB1-induced toxicity. paper, we preliminarily assessed the potential anti-AFB1 activity of a natural polyphenol, quercetin (QUE), BFH12 cells. To end, primarily...

Abstract Aflatoxin B1 (AFB1) is a pro-carcinogenic compound bioactivated in the liver by cytochromes P450 (CYPs). In mammals, CYP1A and CYP3A are responsible for AFB1 metabolism, with formation of genotoxic carcinogens AFB1-8,9-epoxide AFM1, detoxified metabolite AFQ1. Due to climate change, cereals contamination arose Europe. Thus, cattle, as other farm animals fed grains (pig, sheep broiler), more likely exposed via feed consequent release AFM1 milk, posing great concern human health....

This investigation provides for the first time a general view of prescribing patterns antimicrobials in small animal practice Emilia Romagna, Italy. In context project on antimicrobial resistance managed by Regional Veterinary Service, veterinary clinicians were invited to voluntarily complete an online questionnaire. was designed gather information practices and biosecurity measures understand perception issue specific this region total, 266 questionnaires correctly completed collected....

To determine the pharmacokinetics of cefovecin sodium after SC administration to Hermann's tortoises (Testudo hermanni).23 healthy adult (15 males and 8 females).Cefovecin (8.0 mg/kg) was injected once in subcutis neck region tortoises, blood samples were obtained at predetermined time points. Plasma concentrations measured via ultraperformance liquid chromatography coupled tandem mass spectrometry, pharmacokinetic parameters calculated with a noncompartmental model. protein concentration...

Vitamin B12 is a water-soluble molecule composed of tetrapyrrolic complex with cobalt atom at its centre. It an essential regulatory element, synthesized only by bacteria; for this reason it present in food animal origin and the daily requirement humans about 1 to 2 mg. Since milk dairy products provide significant dietary cobalamin intake, ultra performance liquid chromatographytandem mass spectrometry method was applied samples collected different stages along process cheese making order...

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are pollutants of anthropic origin with possible side effects on human health. Diet, in particular fish seafood, is considered the major intake pathway for humans. The present study investigated levels perfluorooctane sulfonate (PFOS) perfluorooctanoic acid (PFOA) contamination twenty-five samples fresh fillet five widely consumed species purchased from large retailers Italy, to be used an estimation Italian population exposure these...