A5041178823- Methane Hydrates and Related Phenomena
- Seismic Waves and Analysis
- Seismology and Earthquake Studies
- Earthquake Detection and Analysis
- Geophysical Methods and Applications
- Ion Transport and Channel Regulation
- Metabolomics and Mass Spectrometry Studies
- Erythrocyte Function and Pathophysiology
- Genetics, Bioinformatics, and Biomedical Research
- Ion Channels and Receptors
- Biomedical Text Mining and Ontologies
- Electrolyte and hormonal disorders
- Physics of Superconductivity and Magnetism
- Cardiac Arrhythmias and Treatments
- Advanced Electron Microscopy Techniques and Applications
- Geomagnetism and Paleomagnetism Studies
- Quantum and electron transport phenomena
- Lipid Membrane Structure and Behavior
- Silicon Carbide Semiconductor Technologies
- Ion channel regulation and function
- Genetics, Aging, and Longevity in Model Organisms
- GABA and Rice Research
- Plant Stress Responses and Tolerance
- Advancements in Semiconductor Devices and Circuit Design
- Hormonal Regulation and Hypertension
Oregon Health & Science University
2018-2024
Vollum Institute
2019-2024
The epithelial sodium channel (ENaC), a member of the ENaC/DEG superfamily, regulates Na+ and water homeostasis. ENaCs assemble as heterotrimeric channels that harbor protease-sensitive domains critical for gating channel. Here, we present structure human ENaC in uncleaved state determined by single-particle cryo-electron microscopy. ion is composed large extracellular domain narrow transmembrane domain. reveals assembles with 1:1:1 stoichiometry α:β:γ subunits arranged counter-clockwise...
Mechanotransduction is the process by which a mechanical force, such as touch, converted into an electrical signal. Transmembrane channel-like (TMC) proteins are evolutionarily conserved family of membrane whose function has been linked to variety mechanosensory processes, including hearing and balance sensation in vertebrates locomotion Drosophila . TMC1 TMC2 components ion channel complexes, but molecular features that tune these complexes diverse stimuli unknown. Caenorhabditis elegans...
The molecular bases of heteromeric assembly and link between Na + self-inhibition protease-sensitivity in epithelial sodium channels (ENaCs) are not fully understood. Previously, we demonstrated that ENaC subunits – α, β, γ assemble a counterclockwise configuration when viewed from outside the cell with protease-sensitive GRIP domains periphery (Noreng et al., 2018). Here describe structure resolved by cryo-electron microscopy at 3 Å. We find combination precise domain arrangement...
Chromatography is an essential family of assays for molecular biology and chemistry. Typically, only a qualitative assessment peak height, position, shape are sufficient to proceed. Additionally, chromatography instrument software proprietary often locked single computer, making data analysis sharing difficult. Since each manufacturer reports the in their own format, performing experiments which use multiple instruments or between labs also challenging. Here we present Appia, free,...
Abstract Mechanotransduction is the process by which a mechanical force, such as touch, converted into an electrical signal. Transmembrane channel-like (TMC) proteins are evolutionarily-conserved family of ion channels whose function has been linked to variety mechanosensory processes, including hearing and balance sensation in vertebrates locomotion Drosophila . The molecular features that tune homologous TMC channel complexes diverse stimuli unknown. Caenorhabditis elegans express two...
Single-particle cryo-electron microscopy enables structure determination of biological molecules and complexes at near atomic resolution with unprecedented speed.Yet in many instances, acquisition a sufficient number particles requires collection thousands micrographs, collected using direct electron detectors comprised dose-fractionated "movie" frames.To achieve the highest reconstructions, user must search grid for optimal regions, typically ones thin ice dense yet non overlapping particle...