A5013692226- Methane Hydrates and Related Phenomena
- Earthquake Detection and Analysis
- Seismic Waves and Analysis
- Geophysics and Gravity Measurements
- Erythrocyte Function and Pathophysiology
- Lipid Membrane Structure and Behavior
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience and Neuropharmacology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Spaceflight effects on biology
- Ion Channels and Receptors
- Synthesis and Properties of Aromatic Compounds
- Photosynthetic Processes and Mechanisms
- Tryptophan and brain disorders
- Toxin Mechanisms and Immunotoxins
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Connexins and lens biology
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- CRISPR and Genetic Engineering
Vollum Institute
2019-2024
Oregon Health & Science University
2019-2024
Oregon State University
2023-2024
Abstract The initial step in the sensory transduction pathway underpinning hearing and balance mammals involves conversion of force into gating a mechanosensory channel 1 . Despite profound socioeconomic impacts disorders fundamental biological significance understanding transduction, composition, structure mechanism complex have remained poorly characterized. Here we report single-particle cryo-electron microscopy native transmembrane channel-like protein (TMC-1) isolated from...
Abstract Type A γ-aminobutyric acid receptors (GABA Rs) are the principal inhibitory in brain and target of a wide range clinical agents, including anaesthetics, sedatives, hypnotics antidepressants 1–3 . However, our understanding GABA R pharmacology has been hindered by vast number pentameric assemblies that can be derived from 19 different subunits 4 lack structural knowledge clinically relevant receptors. Here, we isolate native murine containing widely expressed α1 subunit elucidate...
Mechanotransduction is the process by which a mechanical force, such as touch, converted into an electrical signal. Transmembrane channel-like (TMC) proteins are evolutionarily conserved family of membrane whose function has been linked to variety mechanosensory processes, including hearing and balance sensation in vertebrates locomotion Drosophila . TMC1 TMC2 components ion channel complexes, but molecular features that tune these complexes diverse stimuli unknown. Caenorhabditis elegans...
Abstract Bridge-like lipid transport proteins (BLTPs) are an evolutionarily conserved family of that localize to membrane contact sites and thought mediate the bulk transfer lipids from a donor membrane, typically endoplasmic reticulum (ER), acceptor such as cell or organelle 1 . Despite fundamental importance BLTPs for cellular function, architecture, composition, mechanisms remain poorly characterized. Here, we present subunit composition cryo-electron microscopy structure native LPD-3...
Mechanosensory transduction (MT), the conversion of mechanical stimuli into electrical signals, underpins hearing and balance is carried out within hair cells in inner ear. Hair harbor actin-filled stereocilia, arranged rows descending heights, where tips stereocilia are connected to their taller neighbors by a filament composed protocadherin 15 (PCDH15) cadherin 23 (CDH23), deemed ‘tip link.’ Tension exerted on tip link opens an ion channel at shorter thus converting force signal. While...
Type A GABA receptors (GABA Rs) are the principal inhibitory in brain and target of a wide range clinical agents, including anesthetics, sedatives, hypnotics, antidepressants. However, our understanding R pharmacology has been hindered by vast number pentameric assemblies that can be derived from total 19 different subunits lack structural knowledge clinically relevant receptors. Here, we isolate native murine containing widely expressed α 1 subunit, elucidate their structures complex with...
Summary The initial step in the sensory transduction pathway underpinning hearing and balance mammals involves conversion of force into gating a mechanosensory (MT) channel. Despite profound socioeconomic impacts disorders fundamental biological significance understanding MT, composition, structure mechanism MT complex has remained elusive. Here we report single particle cryo-EM native TMC-1 isolated from C. elegans . 2-fold symmetric is composed 2 copies each pore-forming subunit,...
Abstract Mechanotransduction is the process by which a mechanical force, such as touch, converted into an electrical signal. Transmembrane channel-like (TMC) proteins are evolutionarily-conserved family of ion channels whose function has been linked to variety mechanosensory processes, including hearing and balance sensation in vertebrates locomotion Drosophila . The molecular features that tune homologous TMC channel complexes diverse stimuli unknown. Caenorhabditis elegans express two...
Abstract Hearing and balance rely on the conversion of a mechanical stimulus into an electrical signal, process known as mechanosensory transduction (MT). In vertebrates, this is accomplished by MT complex that located in hair cells inner ear. While past three decades research have identified many subunits are important for revealed interactions between these subunits, composition organization functional remains unknown. The major challenge associated with studying its extremely low...