A5065672564- Glioma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Medical Imaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Brain Metastases and Treatment
- Effects of Radiation Exposure
- Nanoplatforms for cancer theranostics
- Traumatic Brain Injury and Neurovascular Disturbances
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Radiopharmaceutical Chemistry and Applications
- MRI in cancer diagnosis
- CNS Lymphoma Diagnosis and Treatment
- Neurological Disorders and Treatments
- Neurological Disease Mechanisms and Treatments
- Radiation Therapy and Dosimetry
- MicroRNA in disease regulation
- Boron Compounds in Chemistry
- Anesthesia and Neurotoxicity Research
- ATP Synthase and ATPases Research
- Radiomics and Machine Learning in Medical Imaging
- S100 Proteins and Annexins
- Lung Cancer Research Studies
- Immune cells in cancer
- Brain Tumor Detection and Classification
Normandie Université
2008-2025
Université de Caen Normandie
2011-2025
Centre National de la Recherche Scientifique
2011-2025
Cyceron
2015-2024
Imagerie et Stratégies Thérapeutiques pour les Cancers et Tissus cérébraux
2021-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2008-2020
Université Claude Bernard Lyon 1
2015
University of Münster
2012
Centre Hospitalier Universitaire de Caen
2012
Roche (France)
2012
Purposes Lymphopenia is extensively studied, but not circulating leucocyte subpopulations, which however have distinct roles in tumor tolerance. Proton therapy has been shown to a lesser impact on the immune system than conventional X-ray radiotherapy through lower dose exposure healthy tissues. We explored differential effects of brain and proton irradiation subpopulations.
ABSTRACT Background Conventional x-ray-based radiotherapy is a standard treatment for patients with brain tumors. However, associated systemic effects like lymphopenia that correlates poor prognosis. Proton therapy has emerged as new radiation strategy, given the lower entry dose and absence of exit can be exploited to spare healthy tissues reduce side-effects caused by inflammation. We evaluated if irradiation protons could circulating leukocytes along other variables in rodent models....
Using multimodal magnetic resonance imaging (MRI), behavioral, and immunohistochemical analyses, we examined pathological changes at the acute, sub-acute, chronic stages, induced by permanent or temporary ischemia in common marmoset. Animals underwent either (pMCAO) 3-h transient (tMCAO) occlusion of middle cerebral artery (MCAO) intraluminal thread approach. MRI scans were performed 1 h, 8, 45 days after MCAO. Sensorimotor deficits assessed weekly up to Immunohistological studies examine...
Tumor hypoxia significantly limits the effectiveness of radiotherapy, as oxygen is crucial for producing cancer-killing reactive species. To address this, we synthesized nanosized faujasite (PBS-Na-FAU) zeolite crystals using clinical-grade phosphate-buffered saline (PBS) solvent, ensuring preserved crystallinity, microporous volume, and colloidal stability. The nanocrystals showed enhanced safety profiles in vitro ex vivo, vivo studies no apparent toxicity to animals. They demonstrated a...
Many studies showed beneficial effects of either erythropoietin (EPO) or mesenchymal stem cells (MSCs) treatment in cerebral ischemia. In addition to a neuroprotective role, not only EPO but also MSC favors neurogenesis and functional recovery. an attempt further improve postischemic tissue repair, we investigated the effect systemic administration MSC, presence EPO, on recovery transient focal ischemia model adult rat. Twenty-four hours after ischemia, rats were divided into four groups,...
Abstract Background Brain metastases (BM) develop frequently in patients with breast cancer. Despite the use of external beam radiotherapy (EBRT), average overall survival is short (6 months from diagnosis). The therapeutic challenge to deliver molecularly targeted therapy at an early stage when relatively few metastatic tumor cells have invaded brain. Vascular cell adhesion molecule 1 (VCAM-1), overexpressed by nearby endothelial during stages BM development, a promising target. aim this...
The individualized care of glioma patients ought to benefit from imaging biomarkers as precocious predictors therapeutic efficacy. Contrast enhanced MRI and [18F]-fluorodeoxyglucose (FDG)–PET are routinely used in clinical settings; their ability forecast the response is controversial. objectives our preclinical study were analyze sensitive µMRI and/or µPET predict efficacy anti-angiogenic chemotherapeutic regimens. Human U87 U251 orthotopic models implanted nude rats. Temozolomide...
Loss of muscle mass and function is a severe complication in patients with stroke that contributes to promoting physical inactivity disability. The deleterious consequences skeletal loss underline the necessity identity molecular mechanisms involved atrophy after cerebral ischemia.Transient focal ischemia (60 minutes) was induced by occlusion right middle artery C57BL/6J male mice. Skeletal muscles were removed 3 days later analyzed for regulation critical determinants homeostasis...
Tumor hypoxia is known to limit the efficacy of ionizing radiations, a concept called oxygen enhancement ratio (OER). OER depends on physical factors such as pO2 and linear energy transfer (LET). Biological pathways, hypoxia-inducible transcription (HIF), might also modulate influence LET OER. Glioblastoma (GB) resistant low-LET radiation (X-rays), due in part hypoxic environment this brain tumor. Here, we aim evaluate vitro whether high-LET particles, especially carbon ion radiotherapy...
The common marmoset ( Callithrix jacchus), a New World monkey, has recently been used as model of focal cerebral ischaemia. Here, we sought to develop stroke in this species using an intraluminal approach occlude the middle artery (MCA). This technically simple procedure allows both transient and permanent ischaemia with minimal morbidity. Ten marmosets underwent either (3 h) or by insertion nylon filament through external carotid up origin MCA. Cerebral blood flow (CBF) was monitored...
Although chronic arterial hypertension (CAH) increases the risk of stroke and severity resultant lesion, it is rarely integrated in preclinical studies. Here, we analyzed impact CAH on acute spatiotemporal evolution ischemic penumbra as defined by perfusion-weighted imaging/diffusion-weighted imaging mismatch. Sequential 7T-MRI examinations were performed from 30 minutes up to 4 hours after permanent cerebral ischemia genetically hypertensive rats (spontaneously rats, SHR),...
Background and Purpose— Endothelins act through 2 receptors, namely, ET A B . In the cerebral circulation, mediates marked prolonged vasoconstriction, its blockade increases blood flow (CBF) reduces ischemic brain damage. However, role of receptors remains unclear. this study we examined, in rats, kinetics expression effects on changes CBF damage after focal ischemia N -methyl- d -aspartate (NMDA)–induced excitotoxic injury. Methods— Rats were subjected to transient (60 minutes) or cortical...
Abstract Despite multiple advances in cancer therapies, patients with glioblastoma (GBM) still have a poor prognosis. Numerous glioma models are used not only for the development of innovative therapies but also to optimize conventional ones. Given significance hypoxia drug and radiation resistance that is widely observed among GBM, establishment reliable method map preclinical human may contribute discovery translation future more targeted therapies. The aim this study was compare hypoxic...
The partial pressure in oxygen remains challenging to map the brain. Two main strategies exist obtain surrogate measures of tissue oxygenation: saturation studied by magnetic resonance imaging (StO2-MRI) and identification hypoxia a positron emission tomography (PET) biomarker with 3-[18F]fluoro-1-(2-nitro-1-imidazolyl)-2-propanol ([18F]-FMISO) as leading radiopharmaceutical. Nonetheless, formal validation StO2-MRI against FMISO-PET has not been performed. objective our studies was compare...
Abstract Background Diacetyl-bis(N4-methylthiosemicarbazone), labeled with 64Cu ( 64 Cu-ATSM) has been suggested as a promising tracer for imaging hypoxia. However, various controversial studies highlighted potential pitfalls that may disable its use selective hypoxic marker. They also the results be tumor location dependent. Here, we first analyzed uptake of Cu-ATSM and less lipophilic counterpart Cu-Cl 2 in over time an orthotopic glioblastoma model. An vitro study was conducted to...
Abstract Lung cancer patients frequently develop brain metastases (BM). Despite aggressive treatment including neurosurgery and external-radiotherapy, overall survival remains poor. There is a pressing need to further characterize factors in the microenvironment of BM that may confer resistance radiotherapy (RT), such as hypoxia. Here, hypoxia was first evaluated 28 biopsies from with non‑small cell lung (NSCLC) BM, using CA-IX immunostaining. Hypoxia characterization (pimonidazole, HIF-1α)...
There has been an increasing interest in recent years the evaluation of neuronal and glial responses to ischemic insult. Some cytokines, including transforming growth factor-β (TGF-β), that are overexpressed after experimental stroke rodents thought be implicated processes lead necrosis. Thus, such cytokines could predict tissue fate humans, although data currently sparse for gyrencephalic species. The current study addressed expression pattern TGF-β1 a nonhuman primate model middle cerebral...
The impact of stroke on white matter is poorly described in preclinical investigations mainly based rodents with a low (WM)/gray ratio. Using diffusion tensor imaging, we evaluated WM alterations and correlated them sensorimotor deficits after the marmoset, nonhuman primate that displays WM/gray ratio close to humans.Marmosets underwent transient brain ischemia (3-hour). Eight serial MRI examinations were made during up 45 days reperfusion. weekly over days. To assess alterations, SD angle...