A5028797434- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Reproductive System and Pregnancy
- Immune Response and Inflammation
- Sperm and Testicular Function
- RNA Interference and Gene Delivery
- Infant Nutrition and Health
- Immunotherapy and Immune Responses
- Microbial infections and disease research
- Animal Genetics and Reproduction
- Reproductive tract infections research
- Food Allergy and Anaphylaxis Research
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Stress Responses and Cortisol
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Reproductive Biology and Fertility
- Cervical Cancer and HPV Research
- Skin and Cellular Biology Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
National University of the Littoral
2015-2024
Experimental Medicine and Biology Institute
2003-2009
Consejo Nacional de Investigaciones Científicas y Técnicas
2008-2009
University of Buenos Aires
2008
Institute of Astronomy and Space Physics
2005
Chlamydia trachomatis is the most common sexually transmitted bacterial infection globally. Currently, there are no vaccines available despite efforts made to develop a protective one. Polymorphic membrane protein D (PmpD) an attractive immunogen candidate as it conserved among strains and target of neutralizing antibodies. However, its high molecular weight complex structure make difficult handle by recombinant DNA techniques. Our aim predict B-cell T-cell epitopes PmpD. A sequence (Genbank...
The shortcomings of Staphylococcus aureus vaccines to control bovine mastitis have been attributed insufficient capacity the induce opsonizing antibodies and stimulate cellular immune responses. Types antigen, administration route adjuvant used in a vaccine formulation identified as critical factors for development opsonic antibodies. Current commercially available Staph. are formulated with Al(OH) 3 oil-based adjuvants. aim this study was evaluate response heifers immunized CP5 whole cell...
The aim of this study was to determine if dietary fatty acids (FA) level or isomeric FA type may affect reproductive parameters in mice.of Mice were fed for 1 month diets differing cisFA (cFA) content FA. Resorption, number fetuses and placental cytokine expression determined sperm acrosome reaction evaluated after induction by calcium ionophore.Mice high fat showed increased fetal resorptions, a decrease interleukin (IL)-4 the first generation an increase tumor necrosis factor-alpha...
This study aimed to determine the clonal relationship among 137 Streptococcus uberis isolates from bovine milk with subclinical or clinical mastitis in Argentina and assess prevalence conservation of pauA sua genes. information is critical for rational design a vaccine prevention caused by S. uberis. The were typed random amplified polymorphic DNA (RAPD) analysis pulsed-field gel electrophoresis (PFGE). exhibited 61 different PFGE types 25 distinct RAPD profiles. Simpson's diversity index...
Nucleic acids-based therapies have recently developed as next-generation agents for treating and preventing viral infection, cancer, genetic disorders, but their use is still limited due to its relatively poor delivery into targeted cells. We designed synthesized new amphiphilic amino acid derivatives (cysteine-based) of low molecular weight, formed by the same pentapeptide (AG2: WWCOO) N-acylated, with different hydrophobic chains containing from 12 18 carbons, named AG2-Cn (N), which...
Abstract The control of the worldwide spread sexually transmitted Chlamydia trachomatis ( Ct ) infection urgently demands development a preventive vaccine. In this work, we designed vaccine based on fragment polymorphic protein D (FPmpD) that proved to be immunogenic enough generate robust systemic and mucosal IgG humoral immune response in two strains mice. We used heterologous prime-boost strategy, including simultaneous administration routes. high titers anti-PmpD antibodies elicited by...
Problem Evaluation of proacrosin/acrosin ability to induce an immune response in male mice after genetic immunization and assessment animal fertility. Method study Mice received 50 μg per a plasmid containing the human proacrosin cDNA ( pSF 2‐ A cro) (control: empty plasmid, 2). The humoral was evaluated by ELISA immunocytochemistry. In vivo fertility assessed mating immunized males with control females. effect antibodies upon Ca +2 ‐ionophore‐induced acrosomal exocytosis (AE) vitro sperm–...
The development of three-dimensional models reconstituted mouse epidermis (RME) has been hampered by the difficulty to maintain murine primary keratinocyte cultures and achieve a complete epidermal stratification. In this study, new protocol is proposed for rapid convenient generation RME, which reproduces accurately architecture normal epidermis. During RME morphogenesis, expression differentiation markers such as keratins, loricrin, filaggrin, E-cadherin connexins was followed, showing...
The significant impact of Chlamydia trachomatis(Ct) infections worldwide highlights the need to develop a prophylactic vaccine that elicits effective immunity and protects host from immunopathological effects Ct infection. aim this study was evaluate based on fragment Polymorphic membrane protein D (FPmpD) C. trachomatis as an immunogen using heterologous DNA prime-protein boost strategy in female mice Three different formulations were evaluated boost: free recombinant FPmpD (rFPmpD) or...