A5030080152- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Immune Cell Function and Interaction
- Adrenal Hormones and Disorders
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- Cardiomyopathy and Myosin Studies
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Immunology Research
- Parasites and Host Interactions
- Leprosy Research and Treatment
- Pharmacological Effects of Natural Compounds
- Eosinophilic Disorders and Syndromes
- Stress Responses and Cortisol
- Diabetes and associated disorders
- Psoriasis: Treatment and Pathogenesis
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Tryptophan and brain disorders
- Immunodeficiency and Autoimmune Disorders
- Galectins and Cancer Biology
- Reproductive System and Pregnancy
- COVID-19 Clinical Research Studies
- Empathy and Medical Education
National University of Rosario
2015-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2025
Instituto de Biología Molecular y Celular de Rosario
2008-2024
Centro Científico Tecnólogico - Rosario
2022-2024
Instituto Cardiovascular de Rosario
2020
Institute of Immunology
2011
National University of the Littoral
2008
Instituto de Investigaciones Biomédicas en Retrovirus y Sida
2001
Hospital de Emergencias Dr. Clemente Álvarez
2001
Institut Curie
1992
To investigate whether differences in the degree of pulmonary tuberculosis lesions could be accompanied by changes pattern circulating cytokines, 29 untreated patients showing mild (n=10), moderate (n=5) or advanced (n=14) disease, and 12 age-matched healthy controls (mean±S.D., 36±15 years) were studied. ELISA methods for evaluation interferon-γ, interleukin-2, interleukin-4, interleukin-10 indicated that all had increased serum levels four cytokines relation to controls. Mean titers...
The relation between men and women suffering pulmonary tuberculosis is 7/3 in favor to males. Sex hormones could be a significant factor for this difference, considering that testosterone impairs macrophage activation pro-inflammatory cytokines production, while estrogens are proinflammatory mediator's inducer. aim of work was compare the evolution male female mice using model progressive disease. BALB/c mice, were randomized into two groups: castrated or sham-operated, infected by...
Oral transmission of Chagas disease has been documented in Latin American countries. Nevertheless, significant studies on the pathophysiology this form infection are largely lacking. The few investigating oral route disregard that inoculation cavity (Oral infection, OI) or by gavage (Gastrointestinal GI) represent different routes, yet both show clear-cut parasitemia and heart parasitism during acute infection. Herein, BALB/c mice were subjected to OI GI using 5x10(4) culture-derived...
SUMMARY Inoculation of Trypanosoma cruzi, Tulahuén strain, into C57BL/6 and BALB/c mice led to an acute infection characterized by marked parasitaemia, myocardial inflammation thymocyte depletion. While showed a progressive lethal disease, partly recovered. To characterize these murine models more effectively, we studied the parasite burden, serum levels major outcome-related cytokines, in vitro features T. cruzi peritoneal macrophages immunophenotype thymic cells. The greater disease...
The cytokine-mediated stimulation of the hypothalamus–pituitary–adrenal (HPA) axis is relevant for survival during bacterial endotoxemia and certain viral infections. However, only limited information available regarding effects endogenous glucocorticoids on parasite diseases. We have studied this issue using, as a model, C57Bl/6 Balb/c mice infected with Trypanosoma cruzi , causal agent Chagas’ disease. These two mouse strains differ in susceptibility to infection parasite. An intense...
SUMMARY Earlier studies in patients with pulmonary TB have revealed a higher production of Th1 cell type cytokines moderate TB, predominant Th2-like responses advanced disease. Given the influence IL-12 T differentiation, as well roles transforming growth factor-beta (TGF-β), nitric oxide and tumour necrosis factor-alpha (TNF-α) immune response against intracellular pathogens, we decided to analyse interferon-gamma (IFN-γ), IL-4, IL-12, TGF-β, TNF-α nitrite concentrations culture...
Our study investigated the circulating levels of factors involved in immune-inflammatory-endocrine-metabolic responses patients with tuberculosis aim uncovering a relation between certain immune and hormonal patterns, their clinical status vitro response. The concentration leptin, adiponectin, IL-6, IL-1β, ghrelin, C-reactive protein (CRP), cortisol dehydroepiandrosterone (DHEA), response (lymphoproliferation IFN-γ production) was evaluated 53 active untreated tuberculosis, 27 household...
Polymerase chain reaction (PCR) as a diagnostic tool for congenital Chagas disease is scarcely used. To assess the usefulness of PCR, study in neonates was carried out to compare PCR with standard methods; that bloodstream parasites by microhematocrit, and/or seroconversion two conventional procedures. Seventeen neonates, born mothers positive Trypanosoma cruzi serology were analyzed serologic, parasitological and molecular procedures, blood samples taken at birth, when aged 2-4 9 months....
Extrathymic CD4+CD8+ double-positive (DP) T cells are increased in some pathophysiological conditions, including infectious diseases. In the murine model of Chagas disease, it has been shown that protozoan parasite Trypanosoma cruzi is able to target thymus and induce alterations thymic microenvironment lymphoid compartment. acute phase, this results a severe atrophy organ early release DP into periphery. To date, effect changes promoted by infection on central tolerance remained elusive....
Introduction Chronic Chagasic Cardiomyopathy (CCC) has an infectious and inflammatory nature. Recent data also suggest association with altered regulation of glucocorticoid (GC)-mediated circuits failing to control systemic inflammation. However, the involvement receptors (GR) their isoforms have been unexplored. Materials methods The expression GR-α/β isoforms, 11β-hydroxysteroid dehydrogenase type-1 (11β-HSD1), cytokines, GC-regulated gene tristetraprolin (TTP) in peripheral blood...
Abstract We have investigated the relationship between cortisol and dehydroepiandrosterone (DHEA) levels immune response to mycobacterial antigens in peripheral venous blood, from a male population of active tuberculosis patients age‐matched healthy controls same sex (HCo). Peripheral blood mononuclear cells were cultured for 36 or 96 h with whole sonicated Mycobacterium (WSA) measurement proliferation, interferon gamma (IFN‐ γ ) interleukin‐10 (IL‐10) culture supernatants. Comparisons on...
Genotyping studies show a polarized geographic distribution of Trypanosoma cruzi lineages in humans. Here, we assessed their along Latin America through an immunological approach designated Western blot (WB) assay with Trypomastigote small-surface antigen (TSSA) I and TSSA II (TSSA-WB). These antigens are expressed by T. (TCI; now TcI) (TCII; reclassified as TcII to TcVI) parasites. TSSA-WB showed good concordance genotyping tests. An unexpected frequency recognition was observed Colombia,...
Previous studies revealed a significant production of inflammatory cytokines together with severe thymic atrophy and thymocyte migratory disturbances during experimental Chagas disease. Migratory activity thymocytes mature T cells seem to be finely tuned by cytokines, chemokines extracellular matrix (ECM) components. Systemic TNF-α is enhanced infection appears crucial in the response against parasite. However, it also seems involved disease pathology, since implicated arrival effector...