A5018079928- Neuroscience of respiration and sleep
- High Altitude and Hypoxia
- Obstructive Sleep Apnea Research
- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- Heart Rate Variability and Autonomic Control
- Adipose Tissue and Metabolism
- Cardiovascular and Diving-Related Complications
- Cancer, Hypoxia, and Metabolism
- Ion channel regulation and function
- Pulmonary Hypertension Research and Treatments
- Neonatal Respiratory Health Research
- Polyamine Metabolism and Applications
- Biochemical effects in animals
- Plant tissue culture and regeneration
- Pregnancy and preeclampsia studies
- Microbial Metabolites in Food Biotechnology
- Transgenic Plants and Applications
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Celiac Disease Research and Management
- Hemoglobin structure and function
- Circadian rhythm and melatonin
- Biochemical Analysis and Sensing Techniques
- Toxin Mechanisms and Immunotoxins
Instituto de Biomedicina y Genética Molecular de Valladolid
2014-2024
Universidad de Valladolid
2014-2024
Consejo Superior de Investigaciones Científicas
1999-2023
Centro de Investigación Biomédica en Red
2010-2019
Instituto de Salud Carlos III
2012-2019
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2012-2019
Unidades Centrales Científico-Técnicas
1999-2010
Institut des Sciences Moléculaires
2008
Centre National de la Recherche Scientifique
2008
Université de Bordeaux
2008
Two homologous sulfur‐rich basic polypeptides form wheat endosperm, so‐called γ 1 ‐purothionin and 2 ‐purothionin, are described. Purification involves extraction with volatile solvents ammonium bicarbonate fractionation followed by reversed‐phase high‐performance liquid chromatography. The complete primary structure of these two has been determined automatic degradation the intact, S‐carboxymethylated γ‐purothionins peptides obtained enzymatic cleavage. ‐Purothionin consist 47 amino acids...
Obstructive sleep apnea (OSA) consists of sleep-related repetitive obstructions upper airways that generate episodes recurrent or intermittent hypoxia (IH). OSA commonly generates cardiovascular and metabolic pathologies defining the obstructive syndrome (OSAS). Literature usually links OSA-associated to IH would cause an oxidative status a carotid body-mediated sympathetic hyperactivity. Because in obese patients those with OSAS are analogous, we used models (24-wk-old Wistar rats) (applied...
cDNA clones encoding three additional serine carboxypeptidases (Ser-CPs) have been isolated from a gibberellic acid-induced barley aleurone library. The deduced Ser-CPs belong to the two-chain subfamily of Ser-CPs; they are synthesized as precursors with putative signal peptide, propeptide, and linker peptide between A B chains. Their identification provides proof for existence more than in cereal grains, and, based on their sequences, may exhibit new substrate specificities. expression...
1. The release of [3H]dopamine (DA) in response to inhibition the Na+ pump or intracellular acid load was studied rabbit carotid bodies (CB) previously incubated with precursor [3H]tyrosine. ionic requirements and involvement specific ion transport systems were investigated. 2. Inhibition pump, by incubating CB ouabain K(+)‐free medium, evokes a DA which requires presence Ca2+ medium is insensitive nisoldipine. This suggests that triggered entry external through Na(+)‐Ca2+ exchange,...
Abstract Caffeine, an unspecific antagonist of adenosine receptors, is commonly used to treat the apnea prematurity. We have defined effects caffeine on carotid body (CB) chemoreceptors, main peripheral controllers breathing, and identified receptors involved. Caffeine inhibited basal ( IC 50 , 210 µ m ) low intensity (PO 2 ≈ 66 mm Hg/30 K + stimulation‐induced release catecholamines from chemoreceptor cells in intact preparations rat CB vitro . Opposite caffeine, 5′‐( N...
A new sulfur-rich basic polypeptide, so called omega-hordothionin, has been isolated from barley endosperm by extractions with NaCl and ammonium bicarbonate followed reverse-phase high performance liquid chromatography. Purified omega-hordothionin was found to be homogeneous SDS/polyacrylamide gel electrophoresis, N-terminal amino-acid sequencing electrospray-ionization mass spectrometric analysis. The complete primary structure of determined automatic degradation the intact molecule...
On the thirty-fifth anniversary of first description O2-sensitive K+ channels in carotid body chemoreceptors O2 sensing remains a salient issue literature. Whereas much has been learned about this subject, important questions such as identity specific channel subtype(s) responsible for by and mechanism(s) which their activities are altered hypoxia have not yet definitively answered. is fundamental biological process necessary acute chronic responses to varying environmental levels allow...
Experimental evidence suggests that chronic intermittent hypoxia (CIH), a major hallmark of obstructive sleep apnea (OSA), boosts carotid body (CB) responsiveness, thereby causing increased sympathetic activity, arterial and pulmonary hypertension, cardiovascular disease. An enhanced circulatory chemoreflex, oxidative stress, NO signaling appear to play important roles in these responses CIH rodents. Since the guinea pig has hypofunctional CB (i.e., it is natural knockout), this study we...
We have defined Ca2+ channel subtypes expressed in rabbit carotid body (CB) chemoreceptor cells and their participation the stimulus-evoked catecholamine (CA) release. currents (I(Ca)) activated at -30 mV, peaked +10 mV were fully blocked by 200 microm Cd2+. L-type channels (sensitive to 2 nisoldipine) carried 21 +/- 2% of total I(Ca). Non-L-type potentials positive -10 carried: N 1 microM omega-conotoxin-GVIA) 16 1% I(Ca), P/Q 3 omega-conotoxin-MVIIC after nisoldipine plus GVIA) 23 3% I(Ca)...
Chemoreceptor cells of the carotid bodies (CB) are activated by hypoxia and acidosis, responding with an increase in their rate neurotransmitter release, which turn increases electrical activity sinus nerve evokes a homeostatic hyperventilation. Studies isolated chemoreceptor have shown that moderate hypoxias ( 46 mmHg) produces smaller depolarisations comparable Ca(2+) transients but much higher catecholamine (CA) release response intact CBs than intense acidic/hypercapnic stimuli (20%...
The association between obstructive sleep apnea (OSA) and metabolic disorders is well-established; however, the underlying mechanisms that elucidate this relationship remain incompletely understood. Since liver a major organ in maintenance of homeostasis, we hypothesize dysfunction plays crucial role pathogenesis associated with (OSA). Herein, explored within liver. Experiments were performed male Wistar rats fed control or high fat (HF) diet (60% lipid-rich) for 12 weeks. Half groups...
Hypoxia increases the release of neurotransmitters from chemoreceptor cells carotid body (CB) and activity in sinus nerve (CSN) sensory fibers, elevating ventilatory drive. According to previous reports, perinatal hyperoxia causes CSN hypotrophy varied diminishment CB function hypoxic response. The present study aimed characterize presumptive hyperoxic damage. Hyperoxic rats were born reared for 28 days 55%-60% O2; subsequent growth (to 3.5-4.5 months) was a normal atmosphere. control (born...
The role played by Na+ channels of carotid body (CB) chemoreceptor cells was investigated studying the effects tetrodotoxin (TTX) on release 3H-labeled catecholamines ([3H]CA) adult rabbit CBs previously incubated with precursor [3H]tyrosine. TTX inhibited partially [3H]CA elicited mild hypoxia (10 or 7% O2) depolarizing incubation medium containing 20 30 mM KCl, but response to more intense (5 2% higher KCl concentration (40 50 mM) not significantly affected. acidic stimuli, either 20% CO2...
Abstract We have assessed the expression, molecular identification and functional role of Na + channels (Na v ) in carotid bodies (CB) obtained from normoxic chronically hypoxic adult rats. Veratridine evoked release catecholamines (CA) an vitro preparation intact CBs animals, response being Ca 2+ ‐dependent sensitive to tetrodotoxin (TTX). TTX inhibited by 25–50% CA graded hypoxia. Immunoblot assays demonstrated presence α‐subunit ( c. 220 kDa) crude homogenates rat CBs, evident...
A new thionin from barley, omega-hordothionin, has been shown to exist in aqueous solution as a mixture of two different isoforms 3:2 ratio, revealed by complete analysis its two-dimensional 1H-nmr spectra. The conformational heterogeneity arises cis-trans isomerism about the Phe 12-Pro 13 peptide bond, where major form corresponds cis conformation. assignment chemical shifts and nuclear Overhauser effects (NOEs) allows detailed comparative their properties, even though calculation...