A5027214307- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Bone Metabolism and Diseases
- Thyroid Disorders and Treatments
- Effects and risks of endocrine disrupting chemicals
- Adipose Tissue and Metabolism
- Bone health and treatments
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Fatty Acid Research and Health
- Environmental Toxicology and Ecotoxicology
- Metabolism, Diabetes, and Cancer
- Birth, Development, and Health
- Regulation of Appetite and Obesity
- Muscle metabolism and nutrition
- Diabetes Management and Research
- Growth Hormone and Insulin-like Growth Factors
- Muscle Physiology and Disorders
- Food composition and properties
- Microbial Metabolites in Food Biotechnology
- PI3K/AKT/mTOR signaling in cancer
- Neonatal Respiratory Health Research
- Pregnancy and preeclampsia studies
Universidade Estadual Paulista (Unesp)
2018-2024
Yacon (Smallanthus sonchifolius) is a native Andean plant rich in phenolic compounds, and its effects on dysmetabolism cardiomyopathy diabetic rats was evaluated. The (10/group) were allocated as follows: C, controls; C + Y, controls treated with leaf extract (YLE); DM, DM YLE. Type 1 diabetes (T1DM) induced by the administration of streptozotocin (STZ; 40 mg-1/kg body weight, single dose, i.p.), groups received 100 mg/kg weight YLE daily via gavage for 30 d. group shows an improvement...
Considering that the αvβ3 integrin plays an important role in tumor metastasis, this study investigated involvement of these pathways mediating triiodothyronine (T3) effects on amphiregulin (AREG) expression. We treated MCF-7 cells with T3 (10 nM) for 1 hour presence or absence inhibitors (RGD peptide), MAPK (PD98059), PI3K (LY294002), and protein synthesis (cycloheximide [CHX]). A control group (C) received no inhibitors. Analyses mRNA expression were done using RT-qPCR Western blot,...
Human adipose tissue-derived stem cells (hASCs) have been subjected to extensive investigation because of their self-renewal properties and potential restore damaged tissues. In the literature, there are several protocols for differentiating hASCs into osteoblasts, but is no report on control cell viability during this process. study, we used osteoblasts derived from patients undergoing abdominoplasty. The were observed at beginning end bone matrix formation, expression proteins involved in...
Adiponectin and leptin, important for metabolic regulation, are synthesized secreted by adipose tissue influenced triiodothyronine (T3) that activates the MAPK/ERK integrin αVβ3 pathways, modulating gene expression. Adipocytes were treated with T3 (10 nM), 1 h, in absence or presence of PD98059 (PD) tetraiodothyroacetic acid (Tetrac), which pathways inhibitors. The cells incubated Adipo Red/Oil Red O reagents, intracellular lipid accumulation [glycerol triacylglycerol (TAG)], MTT,...
To verify the physiological action of triiodothyronine T3 on expression transforming growth factor α (TGFA) mRNA in MCF7 cells by inhibition RNA Polymerase II and MAPK/ERK pathway.The cell line was treated with at a dose (10-9M) for 10 minutes, 1 4 hour (h) presence or absence inhibitors, α-amanitin (RNA polymerase inhibitor) PD98059 (MAPK/ERK pathway inhibitor). TGFA analyzed RT-PCR. For data analysis, we used ANOVA, complemented Tukey test Student t-test, minimum significance 5%.T3...
Background: Thyroid hormones play a significant role in bone development and maintenance, with triiodothyronine (T3) particularly being an important modulator of osteoblast differentiation, proliferation, maintenance. However, details the biological processes (BPs) molecular pathways affected by T3 osteoblasts remain unclear. Methods: To address this issue, primary cultures human adipose-derived mesenchymal stem cells were subjected to our previously established osteoinduction protocol,...
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