A5034788057- Thyroid Disorders and Treatments
- Regulation of Appetite and Obesity
- Growth Hormone and Insulin-like Growth Factors
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Adipokines, Inflammation, and Metabolic Diseases
- Ophthalmology and Eye Disorders
- Cancer, Hypoxia, and Metabolism
- Marine Biology and Environmental Chemistry
- Glaucoma and retinal disorders
- Environmental Toxicology and Ecotoxicology
- Biochemical Analysis and Sensing Techniques
- Cancer Cells and Metastasis
- Estrogen and related hormone effects
- Effects and risks of endocrine disrupting chemicals
- Genetic and phenotypic traits in livestock
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- Bone health and treatments
- Retinoids in leukemia and cellular processes
- Thyroid Cancer Diagnosis and Treatment
- Bone Metabolism and Diseases
- Metabolism, Diabetes, and Cancer
- Bone and Dental Protein Studies
- PI3K/AKT/mTOR signaling in cancer
Universidade Estadual Paulista (Unesp)
2015-2024
Clinics Hospital of Ribeirão Preto
2022
Universidade de São Paulo
2013-2018
Medicina
2018
University of Naples Federico II
2017
Clinical Research Management
2011
BackgroundThe diagnosis of cancer in Bethesda III/IV thyroid nodules is challenging as fine-needle aspiration (FNA) has limitations, and these cases usually require diagnostic surgery. As approximately 77% are not malignant, a test accurately identifying benign can reduce "potentially unnecessary" surgery rates. We have previously reported the development validation microRNA-based classifier (mir-THYpe) with high sensitivity specificity, which could be performed directly from FNA smear...
This study was designed to determine the genotoxicity of a supraphysiological dose triiodothyronine (T3) in both obese and calorie-restricted animals. Fifty male Wistar rats were randomly assigned one two following groups: control (C; n = 10) (OB; 40). The C group received standard food, whereas OB fed hypercaloric diet for 20 weeks. After this period, half animals (n 20) subjected 25%-calorie restriction 8 weeks forming thus new (OR), remaining kept on initial diet. During weeks, 10 OR...
The present study aimed to examine the effects of thyroid hormone (TH), more precisely triiodothyronine (T3), on modulation leptin mRNA expression and involvement phosphatidyl inositol 3 kinase (PI3K) signaling pathway in adipocytes, 3T3-L1, cell culture. We examined this mediating TH by treating 3T3-L1 adipocytes with physiological (P=10nM) or supraphysiological (SI=100 nM) T3 dose during one hour (short time), absence presence PI3K inhibitor (LY294002). any treatment was considered control...
High expression levels of hypoxia inducing factor 1 alpha are related to mammary carcinogenesis. In previous studies, we demonstrated that transforming growth increases upon treatment with triiodothyronine, but this does not occur in cellular models do express the estrogen receptor, or when cells co-treated anti-estrogen, tamoxifen. The aim study was determine effect hormone triiodothyronine on genes HIF1A and TGFA breast cancer cell line MCF7. subjected at supraphysiological dose 10− 8 M...
Objective To study the effect of different doses triiodothyronine on gene expression adipokines leptin and adiponectin, at times, to evaluate difference in between two each group. Methods 3T3-L1 adipocytes were incubated with physiological dose (10nM) supraphysiological (100nM or 1,000nM), without (control, C) for 0.5, 6, 24 hours. Leptin adiponectin mRNA was detected using real-time polymerase chain reaction (RT-PCR). One-way analyses variance, Tukey’s test Student’s t test, used analyze...
Considering that the αvβ3 integrin plays an important role in tumor metastasis, this study investigated involvement of these pathways mediating triiodothyronine (T3) effects on amphiregulin (AREG) expression. We treated MCF-7 cells with T3 (10 nM) for 1 hour presence or absence inhibitors (RGD peptide), MAPK (PD98059), PI3K (LY294002), and protein synthesis (cycloheximide [CHX]). A control group (C) received no inhibitors. Analyses mRNA expression were done using RT-qPCR Western blot,...
lncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity the mechanisms action these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently identified most enriched transcript mouse developing thyroid. Due its genomic localization antisense protein-encoding Klhl14 gene, we named it Klhl14-AS. In this paper, highlight that Klhl14-AS produces at...
Graves’ ophthalmopathy (GO) is one of the most severe clinical manifestations disease (GD), and its treatment might involve high-dose glucocorticoid therapy. The higher incidence GO among females, reported association between polymorphisms estrogen receptor (ER) GD susceptibility have led us to question role in pathogenesis. We, thus, assessed receptor-alpha (ERA) gene expression cultures orbital fibroblasts from a patient with before (controls) after 10 nM 100 dexamethasone (DEX). Orbital...
Human adipose tissue-derived stem cells (hASCs) have been subjected to extensive investigation because of their self-renewal properties and potential restore damaged tissues. In the literature, there are several protocols for differentiating hASCs into osteoblasts, but is no report on control cell viability during this process. study, we used osteoblasts derived from patients undergoing abdominoplasty. The were observed at beginning end bone matrix formation, expression proteins involved in...
Abstract Adipose tissue (AT), an endocrine organ that modulates several physiological functions by synthesizing and releasing adipokines such as adiponectin, is a metabolic target of triiodothyronine (T3). T3 adiponectin play important roles in controlling normal stimulation fatty acid oxidation increase thermogenesis. The phosphatidylinositol 3‐kinase (PI3K) pathway for the differentiation preadipocytes into adipocytes can be activated transcription specific genes, adiponectin. We examined...
Objective The present study aimed to examine the effects of thyroid hormone (TH), more precisely triiodothyronine (T3), on modulation TH receptor alpha (TRα) mRNA expression and involvement phosphatidyl inositol 3 kinase (PI3K) signaling pathway in adipocytes, 3T3-L1, cell culture. Materials methods: It was examined PI3K mediating T3 by treating 3T3-L1 adipocytes with physiological (P=10nM) or supraphysiological (SI =100 nM) doses during one hour (short time), absence presence inhibitor...
OBJECTIVE: To examine the effect of different doses triiodothyronine (T3) on mRNA levels thyroid hormone receptors, TRα and TRβ, at times. MATERIALS AND METHODS: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), without (control, C) for 0.5, 1, 6, 24h. TRβ was detected using real-time polymerase chain reaction. RESULTS: F increased 0.5h. After 1h, decreased SII compared C, F, SI. 6h, both genes suppressed all concentrations. In 24h,...
Adiponectin and leptin, important for metabolic regulation, are synthesized secreted by adipose tissue influenced triiodothyronine (T3) that activates the MAPK/ERK integrin αVβ3 pathways, modulating gene expression. Adipocytes were treated with T3 (10 nM), 1 h, in absence or presence of PD98059 (PD) tetraiodothyroacetic acid (Tetrac), which pathways inhibitors. The cells incubated Adipo Red/Oil Red O reagents, intracellular lipid accumulation [glycerol triacylglycerol (TAG)], MTT,...
To verify the physiological action of triiodothyronine T3 on expression transforming growth factor α (TGFA) mRNA in MCF7 cells by inhibition RNA Polymerase II and MAPK/ERK pathway.The cell line was treated with at a dose (10-9M) for 10 minutes, 1 4 hour (h) presence or absence inhibitors, α-amanitin (RNA polymerase inhibitor) PD98059 (MAPK/ERK pathway inhibitor). TGFA analyzed RT-PCR. For data analysis, we used ANOVA, complemented Tukey test Student t-test, minimum significance 5%.T3...
The anti-obesity thyroid hormone, triiodothyronine (T3), and irisin, an exercise- and/or cold-induced myokine, stimulate thermogenesis energy consumption while decreasing lipid accumulation. involvement of ATP signaling in adipocyte cell function obesity has attracted increasing attention, but the crosstalk between purinergic cascade hormones lacks experimental evidence. In this study, we investigated effects T3 irisin transcriptomics membrane-bound purinoceptors, ectonucleotidase enzymes...
The current study was aimed at analyzing sarcoplasmic reticulum Ca2+ ATPase (Serca2) and ryanodine receptor type 2 (Ryr2) gene expression in rats subjected to surgery that induced HF were subsequently treated with T4 using physiological doses.HF 18 male Wistar by clipping the ascending thoracic aorta generate aortic stenosis (HFS group), while control group (9-sham) underwent thoracotomy. After 21 weeks, HFS subdivided into two subgroups. One (9 rats) received 1.0 µg of T4/100 g body weight...