A5045837490- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Ocular Surface and Contact Lens
- Connexins and lens biology
- Biochemical effects in animals
- Nutritional Studies and Diet
- Retinal Development and Disorders
- Intraocular Surgery and Lenses
- Advanced Glycation End Products research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Nitric Oxide and Endothelin Effects
- Aortic aneurysm repair treatments
- Collagen: Extraction and Characterization
- Bee Products Chemical Analysis
- Ocular Infections and Treatments
- Redox biology and oxidative stress
- Engineering Technology and Methodologies
- Aortic Disease and Treatment Approaches
- Pregnancy-related medical research
- Infective Endocarditis Diagnosis and Management
- Sulfur Compounds in Biology
- Photoreceptor and optogenetics research
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
Columbia University
2013-2022
Columbia College
2018
Royal College of Physicians
2018
Abington Memorial Hospital
2017
National Institutes of Health
2012-2016
October 6 University
2016
Research to Prevent Blindness
2016
Uijeongbu St. Mary's Hospital
2016
Catholic University of Korea
2016
Morristown Medical Center
2015
The changes in the mechanical integrity of cervix during pregnancy have implications for a successful delivery. Cervical collagens are known to remodel extensively mice with progressing gestation leading soft at term. During this process, mature crosslinked hypothesized be replaced immature less facilitate cervical softening and ripening. To determine role collagen crosslinks normal mouse remodeling, tensile load-to-break tests were conducted following time points: nonpregnant (NP), day (d)...
Abstract Aims Bioprosthetic heart valves (BHVs), made from glutaraldehyde-fixed heterograft materials, are subject to more rapid structural valve degeneration (SVD) in paediatric and young adult patients. Differences blood biochemistries propensity for disease accelerate SVD these patients, which results multiple re-operations with compounding risks. The goal of this study is investigate the mechanisms BHV biomaterial present models studying patients juvenile animal models. Methods We...
Cured meats are high in nitrites. Nitrites generate reactive nitrogen species that may cause nitrative and nitrosative damage to the lung resulting emphysema.To test hypothesis frequent consumption of cured is associated with lower function increased odds chronic obstructive pulmonary disease (COPD).Cross-sectional study 7,352 participants Third National Health Nutrition Examination Survey, 45 years age or more, who had adequate measures meat, fish, fruit, vegetable intake, spirometry.After...
Soft collagenous tissues that are loaded in vivo undergo crosslinking during aging and wound healing. Bioprosthetic implanted also commonly crosslinked with glutaraldehyde (GA). While changes the mechanical properties of tissue, nature underlying microstructural mechanism poorly understood. In this study, a combined mechanical, biochemical simulation approach was employed to identify by which alters properties. The model tissue used an anisotropic cell-compacted collagen gel, agent monomeric...
Pathogenesis of the abdominal aortic aneurysm has been attributed to neovascularization wall. However, it is not clear whether angiogenesis persists in aneurysm. In sections aneurysms, we determined immunohistochemical distributions αvβ3 integrin, tenascin and endothelial nitric oxide synthase (eNOS), which are markers respectively, angiogenesis, matrix remodeling vasoregulatory function. addition, used reverse transcription followed by situ PCR, determine distribution αv mRNA. All specimens...
The efficacy of therapeutic cross-linking the cornea using riboflavin photochemistry (commonly abbreviated as CXL) has caused its use to become widespread. Because there are known chemical agents that cross-link collagenous tissues, it may be possible tissue pharmacologically. present study was undertaken compare cell toxicity such agents.
Second harmonic generation signals (SHG) are emitted preferentially from collagenous tissue structures and have been used to evaluate photochemically-induced (CXL) crosslinking changes in the cornea. Since therapeutic (TXL) using sodium hydroxymethylglycinate (SMG) of sclera is a potential treatment for high myopia, we explored use SHG microscopy effects.Single sub-Tenon's (sT) injections (400 μL) SMG (40-400 mM) were made at equatorial 12 o'clock position right eye cadaveric rabbit heads (n...
Nitrite ion is a by-product of nitrogen oxides (nitric oxide and dioxide) from cigarette smoke used as preservative for curing meats. Therefore, study the reaction nitrite with elastin in vitro was undertaken. By colorimetric assay, reactivity insoluble at neutral pH, 37±C, physiologic concentration confirmed. In histochemical studies on situ human aortic elastin, nitrite-treated sections displayed marked structural disruptions. Determinations fluorescence absorbance soluble bovine revealed...
The effects of age seen in long-lived connective tissue proteins are thought to be the result post-translational modifications by reactive molecules. One such molecule is nitrite ion. Human exposure results predominately from endogenous production nitric oxide as well inhalation cigarette smoke and ingestion cured meats. Although reactions with various have been studied previously regard carcinogenesis, specific reaction collagen its role age-related damage has never examined. We describe...
purpose. Corneal collagen cross-linking through UVA-riboflavin photochemistry (UVAR) has been shown to be an effective treatment for keratoconus and related keratectasias. In recent studies using sclera, the authors observed that short-chain aliphatic β-nitro alcohols can cross-link collagenous tissue under physiologic conditions. Thus, this study was undertaken evaluate these agents as potential pharmacologic alternatives UVAR. methods. Porcine corneal strips (8 × 4 mm) corneoscleral...
Purpose.: Previously, aliphatic β-nitroalcohols (BNAs) have been studied as a means to chemically induce tissue cross-linking (TXL) of cornea and sclera. There are number related possibly more potent agents, known formaldehyde releasers (FARs), that in commercial use preservatives cosmetics other personal care products. The present study was undertaken order screen such compounds for potential clinical utility therapeutic TXL agents.
Current literature contains scant information regarding the extent of enzymatic collagen cross-linking in keratoconus (KC) cornea. The aim present study was to examine levels lysyl oxidase-derived cross-links stromal KC tissue, and correlate cross-link with fibril stability as determined by thermal denaturation temperature (Tm).Surgical samples (n = 17) Eye-Bank control 11) corneas age 18 68 years were analyzed. defatted, reduced (NaBH4), hydrolyzed (6N HCl at 110°C for hours), cellulose...
Features of autoimmunity in abdominal aortic aneurysm (AAA) have been described, including increases IgG content. The present experiments were carried out to determine (1) whether the are subclass specific and (2) complex is associated with an increase isoforms complement C3. Seven AAA, four athero-occlusive (AOD), two normal (NL) tissue extracts evaluated for immunoreactive (C3) components, both by ELISA Western immunoblots (probed a polyclonal goat anti-human C3). also assayed each...
Abstract— The young human lens contains a small metabolite from tryptophan called the O ‐glucoside of 3‐hydroxykynurenine (3‐HKG). Its function is to absorb most radiation between 295 and 400 nm, preventing it reaching retina. With age concentration this component decreases while crystallins acquire covalently attached chromophores. This study investigates photochemical attachment 3‐HKG α‐crystallin. Initial studies showed that α‐crystallin photolyzed in presence developed fluorescence...
Purpose.: The recent tissue cross-linking studies indicate that aliphatic β-nitroalcohols (BNAs) may be useful as pharmacologic corneoscleral agents. present study was performed to identify the specific chemistry involved under physiologic conditions, with intent of identifying more effective Methods.: mechanism chemical at pH 7.4 and 37°C studied using three techniques. colorimetric Griess assay used follow release nitrite from mono-nitroalcohols (2-nitroethanol [2ne], 2-nitro-1-propanol...
Cumulative damage to long-lived connective tissue proteins play a key role in the development of age-related human diseases such as cardiovascular stiffening and macular degeneration. The processes that result accumulation increasingly insoluble, undigestible damaged collagen are only partially known. Nonenzymatic glycation (NEG) is one process has been linked diabetic-related complications aging. An additional novel mechanism particularly relevant smoking- inflammation-related involves...
Purpose: Extracellular matrix (ECM) plays an important role in the regulation of cell function. The aging process may involve chemical modifications to ECM proteins, which contribute Bruch membrane and pathogenesis age-related macular degeneration (AMD). purpose this study is investigate nitrite modification basement membrane-like proteins on RPE behavior as a model for eye diseases. As comparison, retinal pigment epithelium (RPE) glycolaldehyde-modified matrices (GMM) was also studied....