A5103247218- Soil Carbon and Nitrogen Dynamics
- Mass Spectrometry Techniques and Applications
- Analytical chemistry methods development
- Soil and Water Nutrient Dynamics
- Soil and Unsaturated Flow
- Radiopharmaceutical Chemistry and Applications
- Luminescence and Fluorescent Materials
- Agriculture Sustainability and Environmental Impact
- Molecular Biology Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Soybean genetics and cultivation
- Forensic Toxicology and Drug Analysis
- Heavy metals in environment
- Isotope Analysis in Ecology
- Peatlands and Wetlands Ecology
- Sarcoma Diagnosis and Treatment
- Transplantation: Methods and Outcomes
- Metabolomics and Mass Spectrometry Studies
- SARS-CoV-2 detection and testing
- Salivary Gland Disorders and Functions
- Fire effects on ecosystems
- Biosensors and Analytical Detection
- Water Treatment and Disinfection
- Plant responses to elevated CO2
- Hemoglobin structure and function
University of Waterloo
2018-2025
The direct coupling of solid-phase microextraction (SPME) to mass spectrometry (MS) (SPME-MS) has proven be an effective method for the fast screening and quantitative analysis compounds in complex matrices such as blood plasma. In recent years, our lab developed three novel SPME-MS techniques: SPME-microfluidic open interface-MS (SPME-MOI-MS), coated blade spray-MS (CBS-MS), SPME-probe electrospray ionization-MS (SPME-PESI-MS). high-throughput nature these technologies makes them attractive...
This study presents a novel method for in situ extraction of per- and polyfluoroalkyl substances (PFAS) from intact meat samples using recessed solid phase microextraction (SPME) device coupled with LC-MS/MS. The SPME matrix-compatible coating (HLB-WAX/PAN) the section, exhibited mechanically robust low matrix effects (-13.7-11.1 %). Key parameters influencing efficiency, including time, adsorbent amount, temperature, desorption time were comprehensively optimized. stability PFAS adsorbed...
Solid-phase microextraction (SPME) is a simple and highly effective sample-preparation technique for water analysis. However, the extraction coverage of given SPME device with specific coating can be an issue when analyzing multiple environmental contaminants. Therefore, instead synthesizing one sorbent material dual or functions, we investigated new strategy preparing blades using homogeneous slurry made by mixing three different particles─namely, hydrophobic/lipophilic balanced (HLB),...
Proteomics of human saliva samples was achieved for the first time via biocompatible solid-phase microextraction (bio-SPME) devices. Upon introduction a porogen to conventional C18 coating, porous C18/polyacrylonitrile (PAN) SPME blades were able extract peptides up 3.0 kDa and more than commercial blades. Following Trypsin digestion, salivary proteomic analysis SPME-LC-MS/MS. Seven endogenous proteins consistently identified in all bio-SPME. Taking advantage this strategy, untargeted...
Agricultural activities contribute to greenhouse gas emissions, but agroecosystems can also mitigate emissions by adding recalcitrant carbon sources such as biochar soil. Our goal was understand if decrease in soil amended with manure and (MN) or manure, nitrogen fertilizer, (MNB) than N fertilizer over the longer term. We hypothesized that reduces release of labile reactive from organic amendments thereby reducing CO 2 O temperature, moisture, availability are strongest predictors for...
Improved analytical methods for the metabolomic profiling of tissue samples are constantly needed. Currently, conventional sample preparation often involve biopsy and/or homogenization, which disrupts endogenous metabolome. In this study, solid-phase microextraction (SPME) fibers were used to monitor changes in compounds homogenized and intact ovine lung tissue. Following SPME, a Biocrates AbsoluteIDQ assay was applied make downstream targeted metabolomics analysis confirm advantages vivo...
The application of biochar to increase agricultural productivity has sparked considerable attention, yet our understanding the impact a low dosage on soil health remains limited. It was hypothesized that one-time biochar, combined with manure and/or fertilizer, at would lead improvements in both and crop compared without biochar. established exhibited significantly higher (P < 0.05) levels available nitrogen, while orthophosphate were comparatively lower 0.05). Soil water infiltration...
Modeling plays an important role in predicting the long-term effects of biochar on soil organic carbon dynamics. The objective our study was to apply Century model assess changes temporal amended with manure and nitrogen fertilizer (MN), (MB) or manure, (MNB). We determined that, after 115 years, stocks could not reach a steady state (equilibrium) pre-cultivation levels, regardless amendment type. Our results showed that biennial input (MN) led 84% increase compared 79% (MNB) 70% when...
Warmer atmospheric temperatures (eT) will increase plant nutrient uptake, and elevated CO 2 (eCO ) is expected to enhance growth, whereas a multicomponent eTeCO effect should also be beneficial for agroecosystems. Our goal was understand if single- (eT, eCO or (eTeCO climate effects, predicted southern Ontario, Canada, affect soybean soil properties differently when amended with manure biochar (MB) manure, nitrogen (N) fertilizer, (MNB) compared the addition of N fertilizer (MN). We...
BackgroundMetastatic sarcoma is an aggressive disease with few effective treatment options. Standard of care for limited pulmonary metastasis surgical resection, however micrometastasis are often present and go undetected. Here, we determine the maximal tolerated dose safety doxorubicin delivered via In Vivo Lung Perfusion (IVLP) patients resectable metastases.MethodsThis a phase I escalation study using during IVLP in surgically bilateral metastases from 2017 to 2022. While was resected,...
A considerable interest in the application of biochar to improve agricultural productivity is ongoing, but knowledge on impact a low dosage soil health remains limited. We hypothesized that and one-time blended with manure (or) fertilizer improves crop compared without biochar. found available nitrogen was greater (P = 0.001), orthophosphate lower 0.002) Soil water infiltration increased more than 85% 0.001) when manure. However, blending microbial biomass 0.014). Principal component...
A considerable interest in the application of biochar to improve agricultural productivity is ongoing, but knowledge on impact a low dosage soil health remains limited. We hypothesized that and one-time blended with manure (or) fertilizer improves crop compared without biochar. found available nitrogen was greater (P = 0.001), orthophosphate lower 0.002) Soil water infiltration increased more than 85% 0.001) when manure. However, blending microbial biomass 0.014). Principal component...
A considerable interest in the application of biochar to improve agricultural productivity is ongoing, but knowledge on impact a low dosage soil health remains limited. We hypothesized that and one-time blended with manure (or) fertilizer improves crop compared without biochar. found available nitrogen was greater (P = 0.001), orthophosphate lower 0.002) Soil water infiltration increased more than 85% 0.001) when manure. However, blending microbial biomass 0.014). Principal component...
A considerable interest in the application of biochar to improve agricultural productivity is ongoing, but knowledge on impact a low dosage soil health remains limited. We hypothesized that and one-time blended with manure (or) fertilizer improves crop compared without biochar. found available nitrogen was greater (P = 0.001), orthophosphate lower 0.002) Soil water infiltration increased more than 85% 0.001) when manure. However, blending microbial biomass 0.014). Principal component...