A5016853007- Photosynthetic Processes and Mechanisms
- Antioxidant Activity and Oxidative Stress
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Spectroscopy and Quantum Chemical Studies
- Spectroscopy and Chemometric Analyses
- Plant tissue culture and regeneration
- Agricultural Practices and Plant Genetics
- Molecular spectroscopy and chirality
- Electrochemical Analysis and Applications
- Tissue Engineering and Regenerative Medicine
- Algal biology and biofuel production
- Plant Genetic and Mutation Studies
- Pineapple and bromelain studies
- Innovation and Socioeconomic Development
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Plant Stress Responses and Tolerance
- Microbial Inactivation Methods
- Plant biochemistry and biosynthesis
- Biopolymer Synthesis and Applications
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
University of Agriculture in Krakow
2015-2025
The first report presenting successful and efficient carrot genome editing using CRISPR/Cas9 system. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas9) is a powerful tool that has been widely adopted in model organisms recently, but not used carrot-a species for vitro culture studies an important health-promoting crop grown worldwide. In this study, the time, we application of system targeted mutagenesis genome. Multiplexing vectors expressing two...
Abstract Carotenoid microcrystals, extracted from cells of carrot roots and consisting 95 % achiral β‐carotene, exhibit a very intense chiroptical (ECD ROA) signal. The preferential chirality crystalline aggregates that consist mostly building blocks is newly observed phenomenon in nature, may be related to asymmetric information transfer the chiral seeds (small amount α‐carotene or lutein) present cells. To confirm this hypothesis, we synthesized several model various carotenoids. Because...
Recent data indicate that modifications to carotenoid biosynthesis pathway in plants alter the expression of genes affecting chemical composition cell wall. Phytoene synthase (PSY) is a rate limiting factor and it may exhibit species-specific organ-specific roles determined by presence psy paralogous genes, importance which often remains unrevealed. Thus, aim this work was elaborate two paralogs model system reveal biochemical changes wall knockout mutants. For purpose, Clustered Regularly...
The new model orange callus line, similar to carrot root, was rich in carotenoids due altered expression of some carotenogenesis-associated genes and possessed unique diversity chromoplast ultrastructure. Callus induced from root segments cultured vitro is usually pale yellow (p-y) poor carotenoids. A unique, non-engineered line dark (d-o) colour developed this work. content carotenoid pigments d-o at the same level as an storage nine-fold higher than p-y callus. Carotenoids accumulated...
The effect of mineral nutrition on the accumulation main health beneficial compounds in carrots, carotenoid pigments, remains ambiguous; here, a model-based approach was applied to reveal which are responsible for variation content carrot cells vitro. For this purpose, carotenoid-rich callus cultured either BI (modified Gamborg B5) or R Murashige and Skoog MS) media modified obtained by exchanging between R. Callus growing medium had abundant carotene crystals dark orange color contrast pale...
Abstract Carotenoid microcrystals, extracted from cells of carrot roots and consisting 95 % achiral β‐carotene, exhibit a very intense chiroptical (ECD ROA) signal. The preferential chirality crystalline aggregates that consist mostly building blocks is newly observed phenomenon in nature, may be related to asymmetric information transfer the chiral seeds (small amount α‐carotene or lutein) present cells. To confirm this hypothesis, we synthesized several model various carotenoids. Because...
Somatic hybridisation in the carrot, as other plant species, enables development of novel plants with unique characteristics. This process can be induced by application electric current to isolated protoplasts, but such electrofusion requires an effective hybrid cell identification method. paper describes non-toxic fluorescent protein (FP) tagging protoplasts which allows discrimination fusion components and hybrids real-time during electrofusion. One four FPs: cyan (eCFP), green (sGFP),...
Genome editing using CRISPR-based tools is a breakthrough technology allowing the generation of mutations precisely in indicated target DNA sequence. In this review we present principles genome CRISPR/Cas9 system and its application to research carrot, one most important horticultural crops. We summarize experimental results validation by knocking out flavanone 3-hydroxylase (F3H) gene critical for anthocyanins biosynthesis. Using model callus tissue accumulating high level these pigments it...