A5067187666- Neurobiology and Insect Physiology Research
- Nerve injury and regeneration
- Cell Adhesion Molecules Research
- Physiological and biochemical adaptations
- Axon Guidance and Neuronal Signaling
- Cellular Mechanics and Interactions
- Medicinal Plants and Neuroprotection
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Ion channel regulation and function
- Mass Spectrometry Techniques and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Antioxidant Activity and Oxidative Stress
- Signaling Pathways in Disease
- Aquaculture disease management and microbiota
- Memory and Neural Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Circadian rhythm and melatonin
- Invertebrate Immune Response Mechanisms
- Analytical Chemistry and Chromatography
- Zebrafish Biomedical Research Applications
- Hydrocarbon exploration and reservoir analysis
- Advanced Proteomics Techniques and Applications
- Calpain Protease Function and Regulation
University of Calgary
2008-2021
Amsterdam Neuroscience
1994
University of Groningen
1993
Vrije Universiteit Amsterdam
1989-1992
ABSTRACT In this study, we operantly conditioned the aerial respiratory behaviour of freshwater snail Lymnaea stagnalis. Aerial respiration in stagnalis is accomplished by spontaneous opening and closing its orifice, pneumostome, at water surface. Weak tactile stimulation pneumostome area, when open, evoked only closure response, which one aspect escape–withdrawal reflex. Pneumostome resulted termination activity. A contingent paradigm was used to condition animals. Stimulation whenever...
Neuropeptides were directly detected in single identified neurons and the neurohemal area of peptidergic (neuroendocrine) systems Lymnaea brain by using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). The samples placed matrix solution ruptured to allow mixing cell contents with solution. After formation crystals, analytes analyzed MALDI-MS. It was surprising that clean spectra produced, displaying extreme sensitivity detection. In one neuroendocrine studied, we...
The freshwater snail Lymnaea stagnalis (L. stagnalis) has served as a successful model for studies in the field of Neuroscience. However, serious drawback molecular analysis nervous system L. been lack large-scale genomic or neuronal transcriptome information, thereby limiting use this unique model.In study, we report 7,712 distinct EST sequences (median length: 847 nucleotides) normalized central (CNS) cDNA library, resulting largest collection data currently available. Approximately 42%...
Abstract Peptide profiles of single neurons in Lymnaea stagnalis were directly characterized by matrix‐assisted laser desorption–ionization mass spectrometry. The analysis was performed after minor pretreatment and without any separation steps. Good‐quality spectra obtained several cell types also other tissues. results compared with the conventional peptide chemical methods. In addition to many known peptides, new peptides identified. method provides opportunities for studying compositions...
A 13.1-kilodalton protein, cysteine-rich neurotrophic factor (CRNF), was purified from the mollusk Lymnaea stagnalis by use of a binding assay on p75 neurotrophin receptor. CRNF bound to with nanomolar affinity but not similar in sequence neurotrophins or any other known gene product. messenger RNA expression highest adult foot subepithelial cells; central nervous system, regulated lesion. The evoked neurite outgrowth and modulated calcium currents pedal motor neurons. Thus, may be involved...
Extracellular matrix (ECM) proteins and their receptors, the integrins, actively participate in control of many fundamental cellular functions developing nervous system, including regulation cell migration, differentiation, survival neurite outgrowth. ECM–integrin interactions mature system are commonly considered to be more static nature little importance neuronal function. In contrast, we demonstrate that integrins ligands capable rapid neuromodulatory actions. Specifically, show integrin...
1. Nerve growth factor (NGF) is a member of family molecules (the neurotrophins) that can regulate the survival and/or outgrowth many vertebrate cells. NGF also induces from Lymnaea neurons under experimental conditions. Recent studies have shown neurotrophins acutely modulate some physiological properties adult neurons. Here we examined actions on high-voltage-activated (HVA) Ca2+ currents in motoneurons. 2. induced dose-dependent and reversible increase HVA within 2 min. 3. The threshold...
The mammalian epidermal growth factor (EGF) is expressed in the developing and adult CNS, it has been implicated control of cell proliferation, differentiation, neurotrophic events. Despite extensive evolutionary conservation EGF motif a range different types proteins, secreted homologs with actions have not reported invertebrates. In this study, we present novel member family EGF-like factors, an homolog from mollusc Lymnaea stagnalis (L-EGF), demonstrate that protein activity. Purified...
Age-dependent impairment in learning and memory functions occurs many animal species, including humans. Although cell death contributes to age-related cognitive pathological forms of aging, deficiencies develop with age even without substantial death. The molecular cellular basis this biological aging process is not well understood but seems involve a decline the brain's capacity for experience-dependent plasticity. To aid resolving issue, we used simple snail appetitive classical...
In many mating systems female reproductive capacity is a limiting resource over which males will compete. As consequence, and females have usually different fitness optimization strategies may give rise to sexual conflict. Since simultaneous hermaphrodites have, in theory, the option mate as male or at any time, conflict occur if partners insist taking same role. Several lines of evidence exists that body size influences gender choice. However, growth invertebrates indeterminate therefore...
Abstract Background Cognitive impairment associated with subtle changes in neuron and neuronal network function rather than widespread death is a feature of the normal aging process humans animals. Despite its broad evolutionary conservation, etiology this not well understood. However, recent evidence suggests existence link between oxidative stress form progressive membrane lipid peroxidation, declining electrical excitability functional decline brain. The current study applies combination...
Age-related changes in reproduction and growth of the pulmonate snail Lymnaea stagnalis were studied animals under standard culture conditions. Longitudinal studies as well cross-sectional done. Egg-layingactivity starts at an age about 2 months. Up to 250 days it increases, older snailsit decreases with age. Cross-sectional showed that increase egg-laying activity is due number lay eggs frequency individuals. The decrease egglayingactivity a individual numberof stop egg laying. Growth...
Abstract Background Previous studies associate lipid peroxidation with long-term memory (LTM) failure in a gastropod model ( Lymnaea stagnalis ) of associative learning and memory. This process involves activation Phospholipase A 2 (PLA ), an enzyme mediating the release fatty acids such as arachidonic acid that form precursor for variety pro-inflammatory metabolites. study investigated effect biologically realistic challenges L. host defense response system on LTM function potential...
Members of the epidermal growth factor (EGF) family are frequently implicated in injury response mammalian nervous system. Although this implication is supported by extensive molecular evidence, it not underpinned conclusive functional data. Recently, we found that expression an EGF homolog from pond snail Lymnaea stagnalis (L-EGF) upregulated after axotomy adult CNS, suggesting a role for molecule CNS. In present study asked whether L-EGF can promote axonal regeneration three types...
Reactive oxygen species (ROS) are essential for normal physiological functioning of the brain. However, uncompensated increase in ROS levels may results oxidative stress. Phospholipase A2 (PLA2) is one key players activated by elevated resulting hydrolysis various products from plasmamembrane such as peroxidized fatty acids. Free acids (FFAs) and acid metabolites often implicated to genesis cognitive impairment. Previously we have shown that age-, experimentally induced stress causes...
Abstract Glucocorticoids (GCs) are rapidly released in response to stress and play an important role the physiological adjustments re-establish homeostasis. The mode of action GCs for coping is mediated largely by steroid binding glucocorticoid receptor (GR), a ligand-bound transcription factor, modulating expression target genes. However, also exert rapid actions that independent transcriptional regulation second messenger signaling. membrane-specific protein transduces signal yet be...
Abstract Peripheral nerve injury triggers complex responses from neuronal as well multiple nonneuronal cell types. These are coordinated by a wide spectrum of secreted and nonsecreted factors, including growth cytokines, adhesion molecules. molecules originate different sources act both locally at the site centrally location bodies. One signal systems frequently implicated in this process is epidermal factor (EGF) family its receptors. Expression members that EGF‐receptors upregulated types...