A5018983390- Neurobiology and Insect Physiology Research
- Cephalopods and Marine Biology
- Marine Biology and Environmental Chemistry
- Zebrafish Biomedical Research Applications
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Physiological and biochemical adaptations
- Neuroendocrine regulation and behavior
- Olfactory and Sensory Function Studies
- Environmental Toxicology and Ecotoxicology
- Marine Bivalve and Aquaculture Studies
- Neuropeptides and Animal Physiology
- Marine Biology and Ecology Research
- Planarian Biology and Electrostimulation
- Genetics, Aging, and Longevity in Model Organisms
- Fish Ecology and Management Studies
- Receptor Mechanisms and Signaling
- Protein Hydrolysis and Bioactive Peptides
- Aquaculture disease management and microbiota
- Biochemical Analysis and Sensing Techniques
- Aquatic Invertebrate Ecology and Behavior
- Spaceflight effects on biology
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
Dalhousie University
2014-2025
Smith Family
2015
Institute of Molecular Biology and Biophysics
2013
Zero to Three
2006
Dickinson College
2000
Wayne State University
1998
Nova Scotia Health Authority
1997
Koltzov Institute of Developmental Biology
1996
Balaton Limnological Institute
1996
Hungarian Academy of Sciences
1996
Summary (I). Gastropods use chemoreception for a wide variety of behaviours including feeding, homing, escape from predators and social reproductive behaviours. Chemoreception is used to locate distant food sources, discriminate between potential foods. Responses chemical stimuli result combination innate experiential factors. cues in mucus trails home. They also home by direct olfactory orientation. Reproductive behaviour gastropods appears involve cues. Evidence exists pheromones...
Abstract Many teleosts including zebrafish, Danio rerio , actively regulate buoyancy with a gas‐filled swimbladder, the volume of which is controlled by autonomic reflexes acting on vascular, muscular, and secretory effectors. In this study, we investigated morphological development zebrafish swimbladder together its effectors innervation. The first formed as single chamber, inflated at 1–3 days posthatching (dph), 3.5–4 mm body length. Lateral nerves were already present demonstrated...
Posthatching growth in the pond snail Lymnaea stagnalis involves approximately a 20-fold increase linear dimensions of ganglia composing central nervous system. Developmental change within population neurons exhibiting serotoninlike immunoreactivity (SLIR) was examined order to explain this cellular terms. The study indicates that at least two factors contribute First, SLIR cells double number from 200-250 hatchlings complement found animals approaching sexual maturity. Much cell occurred...
Teleost fishes have body tissues that are denser than water, causing them to sink. Many teleosts therefore possess a gas-filled swimbladder provides lift, allowing fish attain neutral buoyancy. The importance of the as buoyancy aid during changing sizes over ontogeny and its role in determining swimming depth remain unclear. In this study, we used zebrafish (Danio rerio) investigate changes size shape development examine whether these affect hydrostatic contribution swimming. Our results...
Many teleosts actively regulate buoyancy by using a gas-filled swim bladder, which is thought to be under autonomic control. Here we investigated the bladder in zebrafish determine possible mechanisms of gas-content regulation. Fluorescently labelled phalloidin revealed myocytes that appeared form sphincter at junction pneumatic duct and esophagus. Myocytes also formed thick bands along ventral surface anterior chamber bilaterally posterior chamber. Thinner layers were located elsewhere....
Abstract In the blind cave‐dwelling morph of A. mexicanus , eye degenerates while other sensory systems, such as gustation, are expanded compared to their sighted (surface‐dwelling) ancestor. This study compares development taste buds along jaws each morph. To determine whether cavefish have an altered onset or rate bud development, we fluorescently labeled basal and receptor cells within over a developmental series. Our results show that number increases during in both morphs. The is,...
Abstract In many marine invertebrates, larval metamorphosis is induced by environmental cues that activate sensory receptors and signalling pathways. Nitric oxide (NO) a gaseous molecule regulates in diverse bilaterians. most cases NO inhibits or represses this process, although it functions as an activator some species. Here we demonstrate positively the poriferan Amphimedon queenslandica . High rates of A. normally coralline alga are inhibited inhibitor nitric synthase (NOS) scavenger....
The embryonic development of the catecholaminergic system pond snail, Lymnaea stagnalis, was investigated by using chromatographic and histochemical methods. High performance liquid chromatography suggested that dopamine only catecholamine present in significant concentrations throughout Lymnaea. Dopamine first became detectable at about stage (E) 15 (15% development) then increased amount during early to reach 120-140 fmol per animal around E40. content remained stable mid-embryogenesis...
The freshwater snail Lymnaea stagnalis (L. stagnalis) has served as a successful model for studies in the field of Neuroscience. However, serious drawback molecular analysis nervous system L. been lack large-scale genomic or neuronal transcriptome information, thereby limiting use this unique model.In study, we report 7,712 distinct EST sequences (median length: 847 nucleotides) normalized central (CNS) cDNA library, resulting largest collection data currently available. Approximately 42%...
Abstract The cover image shows the paired olfactory bulbs of an adult female zebrafish (Danio rerio). was obtained through a novel in toto immunohistochemistry protocol explained Braubach, Fine and Croll this issue. tissue labeled with antibodies against molluscan blood protein, keyhole limpet hemocyanin (red), calcium binding calretinin (green). Axons that enter via nerve (bottom) converge on different target areas, which differ their neurochemistry numbers morphologies glomeruli contained...
ABSTRACT In the vertebrate heart intracardiac nervous system is final common pathway for autonomic control of cardiac output, but neuroanatomy this not well understood. study we investigated innervation in a model vertebrate, zebrafish. We used antibodies against acetylated tubulin, human neuronal protein C/D, choline acetyltransferase, tyrosine hydroxylase, nitric oxide synthase, and vasoactive intestinal polypeptide to visualize neural elements their neurotransmitter content. Most neurons...
The cardiac pacemaker sets the heart's primary rate, with discharge controlled by autonomic nervous system through intracardiac ganglia. A fundamental issue in understanding relationship between neural activity and chronotropy is identification of neuronal populations that control cells. To date, most studies neurocardiac have been done mammalian species, where neurons are embedded distributed throughout heart, so they largely inaccessible for whole-organ, integrative studies. Here, we...
Abstract Gastropods have been well studied in terms of early cell cleavage patterns and the neural basis adult behaviors; however, much less is known about development this taxon. Here we reveal a relatively sophisticated larval nervous system well‐studied gastropod, Ilyanassa obsoleta . The present study employed immunocytochemical histofluorescent techniques combined with confocal microscopy to examine cells containing monoamines (serotonin catecholamine), neuropeptides (FMRFamide...
Abstract In our initial effort to study the ontogeny of gastropod nervous system, we used histological techniques examine post‐embryonic development cells which exhibit serotoninlike immunoreactivity in Lymnaea (Croll and Chiasson, J. Comp. Neurol. 230:122–142, '89). The present complements that report by examining embryonic these neurons. first immunoreactive (SLIR) be detected embryos are paired C4 neurons cerebral ganglia. These faintly visible at about 37–38% have already produced axons...
Previous studies have suggested an involvement of catecholamines in the control several larval behaviors, such as feeding, locomotion, and induction settling metamorphosis. In present study we employed aldehyde-induced, blue-green fluorescence to indicate cells within representatives two bivalve families, Pectinidae (Placopecten magellanicus) Mytilidae (Mytilus edulis). Larvae were examined at different stages development before also shortly after settlement. The general distribution...
Male mating behavior of the simultaneous hermaphrodite freshwater snail Lymnaea stagnalis is controlled by a neuronal network that consists various types peptidergic neurons, as well serotonergic cells. In present article, we describe isolation and characterization cDNA clone encodes multipeptide preprohormone expressed in anterior lobe right cerebral ganglion, group neurons principally innervate penial complex. The 219 amino acids length contains 10 copies peptide Ala-Pro-Gly-Trp-Gly....