A5075123256- Lipid Membrane Structure and Behavior
- Peroxisome Proliferator-Activated Receptors
- Metabolism and Genetic Disorders
- Caveolin-1 and cellular processes
- Metabolism, Diabetes, and Cancer
- Photodynamic Therapy Research Studies
- Electrochemical Analysis and Applications
- Nanoplatforms for cancer theranostics
- Protein Interaction Studies and Fluorescence Analysis
- Biochemical effects in animals
- Analytical Chemistry and Sensors
- Lipid metabolism and biosynthesis
- Drug Transport and Resistance Mechanisms
- Diet, Metabolism, and Disease
- Fuel Cells and Related Materials
- Surfactants and Colloidal Systems
- Molecular Sensors and Ion Detection
- Electrochemical sensors and biosensors
- Enzyme Catalysis and Immobilization
- Porphyrin and Phthalocyanine Chemistry
- Pancreatic function and diabetes
- Pesticide and Herbicide Environmental Studies
- Electrostatics and Colloid Interactions
- Dental Erosion and Treatment
- Pesticide Exposure and Toxicity
Universidade Estadual de Maringá
2019-2024
Boston University
2007-2013
Universidade de São Paulo
2000-2009
University School
2007
CD36 is a multifunctional protein that enhances cellular fatty acid (FA) uptake, key step in energy metabolism, and its dysregulation multiple tissue sites central to obesity-linked diabetes, risk factor for atherosclerosis. Although has been implicated FA uptake correlative way, the molecular mechanisms are not known. Their elucidation cells confounded by receptor-mediated of low-density lipoprotein competitive and/or contributive effects other proteins involved transport which include...
We performed detailed biophysical studies of transfer long-chain fatty acids (FAs) from methyl-β-CD (MBCD) to model membranes (egg-PC vesicles) and cells the extraction FA by MBCD. used i) fluorescein phosphatidylethanolamine detect anions arriving in outer membrane leaflet; ii) entrapped pH dyes measure changes after diffusion (flip-flop) across lipid bilayer; iii) soluble fluorescent-labeled binding protein concentration unbound water. dissociated MBCD, bound membrane, underwent flip-flop...
Ectopic expression of caveolin-1 in HEK293 cells enhances FA sequestration membranes as measured by a pH-sensitive fluorescent dye (1). We hypothesized that is due to the enrichment caveolin cytosolic leaflet and its ability facilitate formation lipid rafts buffer high levels. Here we show ec-topic caveolin-3 also results enhanced sequestration. To further discriminate effect caveolins have on transmembrane movement distribution, labeled outer membrane with...
The effect of palmitic acid (PA) and oleic (OA) on electrical parameters planar membranes was studied. We found a substantial difference between the effects PA OA proton transfer. induced small increase in conductance, requiring new technique for estimating proton-mediated currents across low-conductance bilayers which an electrometer is used to measure transmembrane current under virtual short circuit (SCC). Open-circuit voltage SCC were determine leak conductances. caused marked membrane...
Ectopic expression of caveolin-1 in HEK293 cells enhances FA sequestration membranes as measured by a pH-sensitive fluorescent dye (1Meshulam T. Simard J.R. Wharton J. Hamilton J.A. Pilch P.F. Role and cholesterol transmembrane fatty acid movement.Biochemistry. 2006; 45 (PubMed): 2882-2893Crossref PubMed Scopus (72) Google Scholar). We hypothesized that is due to the enrichment caveolin cytosolic leaflet its ability facilitate formation lipid rafts buffer high levels. Here we show ectopic...
This research examines the interaction between human serum albumin (HSA) and various sugar forms (β-D-fructofuranose (FRC), α-D-glucopyranose (GLC), Keto-D-fructose (FRO), Aldehydo-D-glucose (GLO), modified (GLOm)) using fluorescent spectroscopy, molecular docking simulations, dynamics, protein conformational clusters (EnGens), fractionation with conjugate caps (MFCC) quantum biochemistry analysis. We analyze aspects, uncovering energies atoms amino acids. Total energy considers...
An important challenge for both students and teachers of physiology is to integrate the different areas in which physiological knowledge didactically divided. In developing countries, such an issue even more demanding, because budget restrictions often affect program with laboratory classes being first on list when it comes cuts expenses. With aim addressing this kind problem, graduate our department organized a summer course offered undergraduate students. The objective was present systems...
Glioblastoma Multiforme (GBM) is one of the most aggressive types brain tumor. GBM can modulate glutathione (GSH) levels and regulate cellular redox state, which explain its high resistance to chemotherapeutic agents. Photodynamic therapy (PDT) a selective, nontoxic, minimally invasive treatment approved for many cancer. PDT leads cell death mainly by promoting generation reactive oxygen species (ROS). Thus, in current study, with photosensitizer hypericin (Hyp), formulated mixed...
Abstract The transport of palmitic acid (PA) across planar lipid bilayer membranes was measured using a high specific activity [ 14 C]palmitate as tracer for PA. An all‐glass trans chamber employed in order to minimize adsorbance PA onto the surface. Electrically neutral (diphytanoyl phosphatidylcholine) and charged (Azolectin) bilayers were maintained at open electric circuit. We found permeability (8.8±1.9)×10 −6 cm s −1 ( n =15) (10.3±2.2)×10 =5) bilayers. These values fall within...