A5021783609- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Reproductive Biology and Fertility
- Renal and related cancers
- Ovarian function and disorders
- Estrogen and related hormone effects
- Circadian rhythm and melatonin
- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Genetic Syndromes and Imprinting
- Gynecological conditions and treatments
- Endometrial and Cervical Cancer Treatments
- Uterine Myomas and Treatments
- TGF-β signaling in diseases
- Monoclonal and Polyclonal Antibodies Research
- Reproductive Physiology in Livestock
- Ovarian cancer diagnosis and treatment
- Assisted Reproductive Technology and Twin Pregnancy
- Kruppel-like factors research
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Growth Hormone and Insulin-like Growth Factors
- Dietary Effects on Health
- Genetics, Aging, and Longevity in Model Organisms
Yamaguchi University
2015-2025
We investigated the interactions between mural granulosa cells (MGCs) and cumulus (CGCs) during ovulation after LH surge.
We have previously shown that decidualization of human endometrial stromal cells (ESCs) causes a genome-wide increase in the levels acetylation histone-H3 Lys-27 (H3K27ac). also reported distal gene regions, more than 3 kb up- or downstream transcription start sites increased H3K27ac levels. Insulin-like growth factor-binding protein-1 (IGFBP-1) is specific marker and has its upstream region (-4701 to -7501 bp). Here, using luciferase reporter construct containing this IGFBP-1 region, we...
Abstract Background FIGLA is a transcription factor gene which plays critical role in folliculogenesis. Consistent with this, variants have been identified females non‐syndromic primary ovarian insufficiency (POI) both autosomal‐dominant and autosomal‐recessive forms. Case Description We encountered two Japanese sisters who had secondary or amenorrhea at 15 years of age. They were diagnosed as having hypergonadotropic hypoestrogenism markedly low serum anti‐Müllerian hormone values. Outcome...
In ovarian endometriomas (OE), the expression statuses of various steroid hormone receptors are altered compared with their in eutopic endometrium (EE). For example, OE, expressions estrogen receptor 1 (ESR1), which encodes ERα, and progesterone (PGR) downregulated, while ESR2, ERβ, is upregulated. The causes these changes unclear. DNA methylation a specific region gene can result tissue-specific expression. Such regions called tissue-dependent differentially methylated (T-DMRs). We...
The essential glucose transporter GLUT1 is epigenetically upregulated by C/
We previously reported that CCAAT/enhancer-binding protein beta (C/EBPβ) is the pioneer factor inducing transcription enhancer mark H3K27 acetylation (H3K27ac) in promoter and regions of genes encoding insulin-like growth factor–binding protein-1 (IGFBP-1) prolactin (PRL) this contributes to decidualization human endometrial stromal cells (ESCs). Peroxisome proliferator–activated receptor gamma coactivator 1-alpha (PGC-1α; PPARGC1A) a transcriptional known regulate H3K27ac. However, although...
The Wilms tumor suppressor gene (WT1) encodes an essential transcription factor regulating mammalian urogenital development. However, the function of WT1 in human endometrium is still unclear. current study examined involvement regulation IGF-binding protein-1 (IGFBP-1) and prolactin (PRL), which are specific markers decidualization, endometrial stromal cells (ESCs) undergoing decidualization. ESCs isolated from proliferative-phase were incubated with cyclic adenosine monophosphate (cAMP) to...
Abstract Purpose Decidualization is an important event for embryo implantation and successful pregnancy. Impaired decidualization leads to failure miscarriage. However, it unclear how often occurs in infertile women. By analyzing the endometrium at late‐secretory phase, we investigated incidence pathogenesis of among Methods Endometrial dating was performed on endometria obtained phase from 33 more than 2 days delay taken as indication failure. The expression essential transcription factors...
Abstract The ovulatory LH-surge increases Vegf gene expression in granulosa cells (GCs) undergoing luteinization during ovulation. To understand the factors involved this increase, we examined roles of two transcription and epigenetic mechanisms rat GCs. GCs were obtained from rats treated with eCG before, 4 h, 8 12 h 24 after hCG injection. mRNA levels gradually increased injection reached a peak at h. investigate mechanism by which is up-regulated injection, focused on C/EBPβ HIF1α. Their...
We previously reported that the transcription factor Wilms tumor 1 (WT1) regulates expression of insulin-like growth factor-binding protein-1 (IGFBP-1) and prolactin (PRL) during decidualization human endometrial stromal cells (ESCs). However, other roles WT1 in remain to be fully clarified. Here, we investigated how physiological functions ESCs decidualization. incubated isolated from proliferative-phase endometrium with cAMP induce decidualization, knocked down siRNA, generated three types...
Abstract Purpose To identify the upstream regulators (URs) involved in onset and pathogenesis of ovarian endometrioma. Methods Recently, a method called Significance-based Modules Integrating Transcriptome Epigenome (SMITE) that uses transcriptome data combination with publicly available for identifying URs cellular processes has been developed. Here, we used SMITE from endometrioma stromal cells (ovESCs) eutopic endometrium (euESCs) gene regulatory network data. confirm identified by SMITE,...
Melatonin is a promising reagent that can improve assisted reproductive technology (ART) outcomes in infertility patients. However, melatonin not effective for all infertile patients, and it remains unclear which patients would be effective. This study examined the effects of on ART its mechanisms. increased fertilization rate whose rates previous cycle were less than 50%, but more 50% cycle. blastocyst formation embryo development To clarify mechanisms, transcriptome changes by treatment...
Decidualization stimuli activate the insulin signaling pathway and increase glucose uptake in human endometrial stromal cells (ESCs). The inductions of prolactin ( PRL ) IGF-binding protein-1 IGFBP1 ), specific markers decidualization, were inhibited by incubating ESCs under low concentrations. These results suggested that decidualization pathway, which contributes to through uptake. Here, we investigated mechanisms regulates decidualization. incubated with cAMP induce We examined whether...
Abstract The ovulatory luteinizing hormone (LH) surge induces rapid changes of gene expression and cellular functions in granulosa cells (GCs) undergoing luteinization. However, it remains unclear how the genome-wide are regulated. H3K4me3 histone modifications involved alteration expression. In this study, we investigated transcriptome status mouse GCs were obtained from mice treated with equine chorionic gonadotropin (hCG) before, 4 hours, 12 hours after human injection. RNA-sequencing...
We investigate the relationships between oocyte developmental capacity and follicular size of its origin in Japanese women: those undergoing conventional IVF (cIVF) ICSI, respectively.
Decidualization can be induced by culturing human endometrial stromal cells (ESCs) with several decidualization stimuli, such as cAMP, medroxyprogesterone acetate (MPA) or Estradiol (E2). However, it has been unclear how decidualized different stimuli are different. We compared transcriptomes and cellular functions of ESCs (MPA, E2 + MPA, cAMP MPA). also investigated which stimulus induces a closer in vivo decidualization. Differentially expressed genes (DEGs) altered each were identified...
Abstract Purpose To test the theory that invaginated ovarian surface epithelium and endometrial implants on ovary form endometriomas. Methods Adhesion sites of endometrioma peritoneum consecutive cyst wall, called non‐adhesion sites, were histologically examined. DNA methylomes adhesion blueberry spots compared with those ovary, endometrium, peritoneum. Results The showed an epithelium‐like structure near site, which continued to a columnar structure. Calretinin staining was strong in but...
Women usually experience body weight gain with aging, which can put them at risk for many chronic diseases. Previous studies indicated that melatonin treatment attenuates and abdominal fat deposition in several male animals. However, it is unclear whether affects female animals the same way. This study investigated long-term attenuate aging and, if does, what mechanism is. Ten-week-old ICR mice were given melatonin-containing water (100 μg/mL) or only until 43 weeks. Melatonin significantly...
Abstract Decidualization of the human endometrium is critical for establishing pregnancy and entailed by differentiation endometrial stromal cells (ESCs) into decidual cells. During decidualization, actin cytoskeleton dynamically reorganized ESCs’ morphological functional changes. Although alters its polymerized state upon external stimuli not only in cytoplasm, but also nucleus, nuclear dynamics during decidualization have been elucidated. Here, we show that was specifically assembled ESCs....