A5070175544- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Animal Behavior and Welfare Studies
- Reproductive Biology and Fertility
- Estrogen and related hormone effects
- Hypothalamic control of reproductive hormones
- Animal Nutrition and Physiology
- Genetic and phenotypic traits in livestock
- Veterinary Equine Medical Research
- Meat and Animal Product Quality
- Veterinary Medicine and Surgery
- Angiogenesis and VEGF in Cancer
- Inflammatory mediators and NSAID effects
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Growth Hormone and Insulin-like Growth Factors
- Pharmacological Effects and Assays
- Endometrial and Cervical Cancer Treatments
- Neuroendocrine regulation and behavior
- Neuropeptides and Animal Physiology
- Pregnancy-related medical research
- Effects of Environmental Stressors on Livestock
- Virus-based gene therapy research
- Endometriosis Research and Treatment
- Xenotransplantation and immune response
Institute of Animal Reproduction and Food Research
2014-2023
Polish Academy of Sciences
2014-2023
University of Turku
1999-2005
Imperial College London
2005
Pomeranian Medical University
1995
University of Computer Sciences and Economics in Olsztyn
1977-1995
North Carolina State University
1988-1991
North Dakota State University
1981-1987
Institute of Biology and Immunology of Reproduction
1986
Babraham Institute
1980
Contents Establishment of pregnancy in pigs requires continuous function corpora lutea and endometrial preparation for embryo implantation. Progesterone regulates expression many proteins necessary remodelling embryo–maternal communications. Attaining the uterine receptivity involves progesterone priming loss receptors epithelium before days 10–12 after oestrus. Spermatozoa oocytes oviduct alter secretion specific that exert beneficial effect on gametes embryos. Moreover, an appropriate...
Before implantation, the porcine endometrium and trophoblast synthesize elevated amounts of luteoprotective prostaglandin estradiol-17β (E2) (PGE2). We hypothesized that embryo signal, E2, PGE2 modulate expression key enzymes in PG synthesis: PG-endoperoxide synthase-2 (PTGS2), microsomal PGE synthase (mPGES-1), PGF (PGFS), 9-ketoreductase (CBR1) as well receptor (PTGER2 -4) signaling within endometrium. determined site action during estrous cycle pregnancy. Endometrial tissue explants...
LH/hCG-binding sites were measured in crude membrane fractions of porcine uteri. Specific high affinity and low capacity receptors for LH/hCG found all (n = 17) preparations myometrium but only 5 17 endometrium There was very little competition between hCG GH (pGH), bovine TSH, pFSH, pPRL (0.5%, 0.3%, 0.2%, less than 0.005%, respectively). Specificity [125I]hCG binding to other tissues determined by incubating heart, skeletal muscle, liver, kidney. Numbers affinities available characterized...
Endometrial prostaglandins (PGs) and the PGE2/PGF2alpha ratio play an important role in regulating estrous cycle establishment of pregnancy. The enzymes downstream cyclooxygenase-2 may determine porcine uterus. Thus, we have cloned PGF synthase (PGFS) microsomal PGE synthase-1 (mPGES-1) characterized their expression endometrium during early PGFS mPGES-1 amino acid sequences possessed a high degree (>67% >77%, respectively) identity with other mammalian homologs. There was little modulation...
Prostaglandins (PGs) play a pivotal role in luteolysis, maternal recognition of pregnancy, and implantation. In many species, including pigs, both conceptus (embryo associated membranes) endometrium synthesize PGE(2), which may antagonize PGF(2alpha) by playing luteotropic/antiluteolytic role. Previously, we have reported expression profiles PG G/H synthases (PGHS-1 PGHS-2), PGE synthase (mPGES-1), PGF (PGFS) the cyclic pregnant pigs. present study, above-mentioned synthesis enzymes...
Summary. The concentration, affinity constant and occupancy of the LH receptor have been measured in corpora lutea, from 29 pigs at Days 6–16 oestrous cycle, 25 12–30 pregnancy, by using125I-labelled porcine tracer. Investigation specificity showed that cross-reactions other pituitary hormones were accounted for their contamination with LH. Luteal concentration unoccupied receptors doubled between 6 10 decreased 12 14; it increased 3-fold 20 30 but was lower on Day pregnancy than a...
In order to characterize the transition of corpora lutea (CL) from acquisition luteolytic sensitivity rescue luteal function: i) expression 38 factors associated with steroids, prostanoids, and angiogenic systems ii) concentrations main hormones responsible for maintenance CL function in cyclic pregnant pigs were examined. Additionally, effect prostaglandin (PG) E2 F2 α on during estrous cycle pregnancy was evaluated vitro. Significantly up-regulated gene revealed collected day 14 (CYP19A1,...
Contents Cyclooxygenase (COX) is the rate‐limiting enzyme that catalyses initial step in prostaglandins (PGs) production. In present studies, endometrial COX‐1 and COX‐2 expression throughout oestrous cycle early pregnancy was analysed pigs using real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR), Western blot immunohistochemistry. There were no changes messenger RNA (mRNA) protein for cyclic pigs. pregnant animals, mRNA levels of this increased on days 22–25 (p < 0.001)....
The objective of this study was to investigate the protein and mRNA expression vascular endothelial growth factor (VEGF), VEGFR-1 (fms-like tyrosine kinase, Flt-1) VEGFR-2 (fetal liver kinase-1/kinase insert domain-containing receptor, Flk-1/KDR) in endometrium during estrous cycle early pregnancy pigs. VEGF-receptor system localized epithelial stromal cells, blood vessels, myometrium. Western blot analysis showed higher levels VEGF periovulatory periimplantation periods (P < 0.001, P 0.05,...
The current studies investigated the concentration and distribution of LH receptors in oviduct ovariectomized gilts at various times after administration oestradiol benzoate (10 μg kg−1 body weight) to determine whether participates regulation oviductal contractions. Polyclonal antibodies receptor were used immunocytochemical western blot analyses tissues. mechanical activity isthmus ampullar segments oviduct, collected from 16 cyclic gilts, was recorded for 30 min or hCG treatment. In there...
Contents The endometrial tissue of the uterus plays a key role in reproduction and is source hormones factors responsible for proper physiological function reproductive tract during oestrous cycle pregnancy. In this study, we investigated pattern PGF 2 α PGE secretion from cultured porcine cells at different days cycle. Epithelial stromal were isolated by differential enzymatic digestion on 6–8, 10–12 14–16. After attachment incubated 3 24 h to estimate output. purity culture was 85–90%...