A5083505431- Hypothalamic control of reproductive hormones
- Receptor Mechanisms and Signaling
- Estrogen and related hormone effects
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Neuropeptides and Animal Physiology
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- RNA and protein synthesis mechanisms
- Reproductive Physiology in Livestock
- Growth Hormone and Insulin-like Growth Factors
- Neuroscience and Neuropharmacology Research
- Developmental Biology and Gene Regulation
- Virus-based gene therapy research
- Sperm and Testicular Function
- Cell death mechanisms and regulation
- Ovarian function and disorders
- RNA Research and Splicing
- Congenital heart defects research
- Cancer Mechanisms and Therapy
- Immunotherapy and Immune Responses
- Sexual Differentiation and Disorders
- Hedgehog Signaling Pathway Studies
Medical University of Lublin
2016-2025
University of Turku
2013-2025
Åbo Akademi University
2003-2021
Turku Centre for Biotechnology
2003-2018
University of Surrey
2000-2006
G protein–coupled receptors (GPCRs) are ubiquitous mediators of signaling hormones, neurotransmitters, and sensing. The old dogma is that a one ligand/one receptor complex constitutes the functional unit GPCR signaling. However, there mounting evidence some GPCRs form dimers or oligomers during their biosynthesis, activation, inactivation, and/or internalization. This has been obtained exclusively from cell culture experiments, proof for physiological significance di/oligomerization in vivo...
Significance Angiogenesis, the formation of new blood vessels from existing ones, is essential for embryonic development and necessary tumor growth invasion. The Notch signaling pathway regulates angiogenesis where two ligands, Dll4 Jagged 1, exert opposite functions on sprouting. We found that intermediate filament vimentin balances by binding specifically to proangiogenic ligands. This provides a force-generating mechanism Jagged–Notch complex ensure efficient transendocytosis activation....
Abstract The intermediate filament (IF) cytoskeleton has been proposed to regulate morphogenic processes by integrating the cell fate signaling machinery with mechanical cues. Signaling between endothelial cells (ECs) and vascular smooth muscle (VSMCs) through Notch pathway regulates arterial remodeling in response changes blood flow. Here we show that IF-protein vimentin strength hemodynamic forces. Vimentin is important for transactivation ECs knockout mice (VimKO) display disrupted VSMC...
Sirtuin 7 (SIRT7), a member of the sirtuin family NAD+-dependent deacetylases, plays vital role in cancer, exhibiting context-dependent functions across various malignancies. Our study investigates SIRT7 depletion head and neck squamous cell carcinoma (HNSCC) progression. In vitro 3D organotypic models demonstrated that knock-out attenuates cancer viability, proliferation, motility as well induces downregulation migration- epithelial-mesenchymal transition (EMT)-related gene expression....
Endometrial prostaglandins (PGs) and the PGE2/PGF2alpha ratio play an important role in regulating estrous cycle establishment of pregnancy. The enzymes downstream cyclooxygenase-2 may determine porcine uterus. Thus, we have cloned PGF synthase (PGFS) microsomal PGE synthase-1 (mPGES-1) characterized their expression endometrium during early PGFS mPGES-1 amino acid sequences possessed a high degree (>67% >77%, respectively) identity with other mammalian homologs. There was little modulation...
Classically, follicle-stimulating hormone receptor (FSHR)-driven cAMP-mediated signaling boosts human ovarian follicle growth and oocyte maturation. However, contradicting in vitro data suggest a different view on physiological significance of FSHR-mediated cAMP signaling. We found that the G-protein-coupled estrogen (GPER) heteromerizes with FSHR, reprogramming cAMP/death signals into proliferative stimuli fundamental for sustaining survival. In granulosa cells, survival are missing at high...
Keratins (K) are intermediate filament proteins important in protection from cellular stress. K8, K18 and K19 the main components of keratin filaments colonic epithelia but their role intestinal diseases remains ambiguous. A function for keratins health is supported by K8-knock-out (K8(-/-)) mouse which manifests an early chronic ulcerative colitis-like inflammatory bowel disease epithelial hyperproliferation. We tested whether K8(-/-) mice more susceptible to colorectal cancer (CRC)...
Mutagenesis is an important tool to study gene regulation, model disease-causing mutations and for functional characterisation of proteins. Most the current methods mutagenesis involve multiple step procedures. One most accurate genetically altering DNA recombineering, which uses bacteria expressing viral recombination Recently, use in vitro seamless assembly systems using purified enzymes multiple-fragment cloning as well gaining ground. Although these isothermal reactions are useful when...
Functional genomics require manipulation and modification of large fragments the genome. Such has only recently become more efficient due to discovery different techniques based on homologous recombination. However, certain limitations these strategies still exist since insertion homology arms (HAs) is often amplification DNA sequences with PCR. Large quantities PCR products longer than 4–5 kb can be difficult obtain risk mutations or mismatches increases size template that being amplified....
Glycoprotein hormones are complex hormonally active macromolecules. Luteinizing hormone (LH) is essential for the postnatal development and maturation of male gonad. Inactivating beta ( LHB ) gene mutations exceptionally rare lead to hypogonadism that particularly severe in males. We describe a family with selective LH deficiency two brothers. DNA sequencing was performed effects genetic variants on function secretion were characterized by mutagenesis studies, confocal microscopy functional...
Colorectal cancer (CRC) is the third leading cause of cancer-related deaths in men and women. The impact new pyrazolo[4,3-e]tetrazolo[1,5-b][1,2,4]triazine sulphonamide (MM-129) was evaluated against human colon vitro zebrafish xenografts. Our results show that this synthesised compound effectively inhibits cell survival BTK-dependent mechanism. Its effectiveness much higher at a relatively low concentration as compared with standard chemotherapy used for CRC, i.e. 5-fluorouracil (5-FU)....
Research on spermatogonia is hampered by complex architecture of the seminiferous tubule, poor viability testicular tissue ex vivo and lack physiologically relevant long-term culture systems. Therefore there a need for an in vitro model that would enable long term survival propagation spermatogonia. We aimed at most simplified approach to all different cell types within tubules contribute creation niche In present study we describe establishment co-culture mouse cells based proliferative...