A5081246344- melanin and skin pigmentation
- Amino Acid Enzymes and Metabolism
- Selenium in Biological Systems
- Genomics, phytochemicals, and oxidative stress
- Biochemical effects in animals
- Redox biology and oxidative stress
- Neuropeptides and Animal Physiology
- Sulfur Compounds in Biology
- Metabolism and Genetic Disorders
- Biochemical Analysis and Sensing Techniques
- Glutathione Transferases and Polymorphisms
- Cervical Cancer and HPV Research
- Alzheimer's disease research and treatments
- Skin Protection and Aging
- Folate and B Vitamins Research
- Phytochemicals and Antioxidant Activities
- Biochemical and Molecular Research
- Down syndrome and intellectual disability research
- Molecular Biology Techniques and Applications
- Heat shock proteins research
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Structure and Function
- Dye analysis and toxicity
- Enzyme Catalysis and Immobilization
- Antioxidant Activity and Oxidative Stress
Institute of Molecular Biology and Pathology
2008-2021
Sapienza University of Rome
1994-2021
National Research Council
2014-2020
Istituto di Chimica Biomolecolare
1982
Abstract Purpose : The present study aims to evaluate a set of oxidative stress biomarkers in the amniotic fluid (AF) women carrying Down syndrome (DS) fetuses that could prove vivo early occurrence damage DS. Experimental design To assess extent protein oxidation DS AF, we measured carbonylation and protein‐bound HNE by slot‐blot analysis, total oxidized GSH levels enzymatic assay heat shock proteins (HSPs) thioredoxin (Trx) induction Western blot. Further, redox proteomics approach...
Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main etio-pathogenetic factor of cervical cancer, is not per se sufficient to induce tumour development. Oxidative stress (OS) represents an interesting and under-explored candidate a promoting in HPV-initiated carcinogenesis. To gain insight into role OS HPV-16 positive tissues were collected from patients with invasive squamous carcinoma, High Grade dysplastic HPV lesions no clinical evidence lesions....
Multiple sclerosis (MS) is an autoimmune inflammatory demyelinating disease of the central nervous system. Several evidences suggest that MS can be considered a multi-factorial in which both genetics and environmental factors are involved. Among proposed candidates, growing results support involvement oxidative stress (OS) pathology. The aim this study was to investigate role OS event exacerbations on serum relapsing-remitting (RR-MS) patients, either relapsing or remitting phase, with...
Increasing evidences support the notion that impairment of intracellular degradative machinery is responsible for accumulation oxidized/misfolded proteins ultimately results in deposition protein aggregates. These events are key pathological aspects "protein misfolding diseases", including Alzheimer disease (AD). Interestingly, Down syndrome (DS) neuropathology shares many features with AD, such as both amyloid plaques and neurofibrillary tangles. Studies from our group others demonstrated,...
Abstract Background The UVB component of solar ultraviolet irradiation is one the major risk factors for development skin cancer in humans. exposure elicits an increased generation reactive oxygen species (ROS), which are responsible oxidative damage to proteins, DNA, RNA and lipids. In order examine biological impact on cells, we used a parallel proteomics approach analyze protein expression profile identify oxidatively modified proteins normal human epithelial keratinocytes. Results levels...
UV solar radiation is the major environmental risk factor for malignant melanoma. A great effort currently posed on search of new compounds able to prevent or reduce UV-mediated cell damage. Ferulic acid a natural compound recently included in formulation protecting dermatological products. The purpose present work was assess whether its ethyl ester derivative, FAEE, could protect skin melanocytes from UV-induced oxidative stress and Experiments human irradiated with UVB showed that FAEE...
Abstract Increasing evidence supports the role of oxidative stress in cancer development. Ultraviolet (UV) irradiation is one major sources through generation reactive oxygen species (ROS). Besides physiological function ROS cellular homeostasis, accumulating reports suggest that are involved all stages multistep In order to investigate involvement damage into mechanisms tumour progression, we used a parallel proteomic approach analyse protein expression profile and identify oxidatively...
The β-amyloid (Aβ) peptide plays a key role in the pathogenesis of Alzheimer's disease. methionine (Met) residue at position 35 Aβ C-terminal domain is critical for neurotoxicity, aggregation, and free radical formation initiated by peptide. Met modulating toxicological properties most likely involves an oxidative event sulfur atom. We therefore investigated one- or two-electron oxidation Aβ25-35 fragment effect such on behavior Bicarbonate promotes oxidations mediated hydrogen peroxide...
Abstract Background Melanin synthesis, the elective trait of melanocytes, is regulated by tyrosinase activity. In tyrosinase-positive amelanotic melanomas this rate limiting enzyme inactive because acidic endo-melanosomal pH. The E5 oncogene Human Papillomavirus Type 16 a small transmembrane protein with weak transforming activity and role during early steps viral infections. has been shown to interact kDa subunit C trans-membrane Vacuolar ATPase proton pump ultimately resulting in its...