A5084517006- Cellular transport and secretion
- Autoimmune Neurological Disorders and Treatments
- CRISPR and Genetic Engineering
- Peripheral Neuropathies and Disorders
- Neural dynamics and brain function
- RNA Interference and Gene Delivery
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Advanced Chemical Sensor Technologies
- Stress Responses and Cortisol
- Human-Animal Interaction Studies
- Autophagy in Disease and Therapy
- Adenosine and Purinergic Signaling
- Memory and Neural Mechanisms
- Genetics and Neurodevelopmental Disorders
- Olfactory and Sensory Function Studies
- Virus-based gene therapy research
- Metabolism, Diabetes, and Cancer
- Genetic Neurodegenerative Diseases
- PI3K/AKT/mTOR signaling in cancer
- MicroRNA in disease regulation
National Hospital for Neurology and Neurosurgery
2018-2023
University College London
2018-2023
University of Genoa
2013-2019
Italian Institute of Technology
2019
Royal College of Surgeons in Ireland
2015
Abstract The ATP-gated ionotropic P2X7 receptor (P2X7R) modulates glial activation, cytokine production and neurotransmitter release following brain injury. Levels of the P2X7R are increased in experimental human epilepsy but mechanisms controlling expression remain poorly understood. Here we investigated responses after focal-onset status epilepticus mice, comparing changes damaged, ipsilateral hippocampus to spared, contralateral hippocampus. P2X7R-gated inward currents were suppressed...
Significance The mTORC1 complex provides a critical role in cell function, regulating variety of processes including growth and autophagy. signaling is hyperactivated range common diseases cancer, epilepsy, neurodegenerative disorders. Hence, an important target for regulation many contexts. Here, we show that decanoic acid, key component widely used medicinal diet, reduces activity. We identify this tractable model system, validate it ex vivo rat brain tissue human iPSC-derived astrocytes...
Neuronal plasticity produces changes in excitability, synaptic transmission, and network architecture response to external stimuli. Network adaptation environmental conditions takes place time scales ranging from few seconds days, modulates the entire dynamics. To study defined long-term experimental protocols, we setup a system that combines optical electrophysiological tools embedded cell incubator. Primary hippocampal neurons transduced with lentiviruses expressing...
Aromatic l-amino acid decarboxylase (AADC) deficiency is a complex inherited neurological disorder of monoamine synthesis which results in dopamine and serotonin deficiency. The majority affected individuals have variable, though often severe cognitive motor delay, with movement high risk premature mortality. For most, standard pharmacological treatment provides only limited clinical benefit. Promising gene therapy approaches are emerging, may not be either suitable or easily accessible for...
Abstract Objective Leucine‐rich glioma‐inactivated 1 (LGI1) is a secreted transsynaptic protein that interacts presynaptically with Kv1.1 potassium channels and disintegrin metalloprotease (ADAM) 23, postsynaptically influences α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionate receptors through direct link the ADAM22 cell adhesion protein. Haploinsufficiency of LGI1 or autoantibodies directed against are associated human epilepsy, generating hypothesis subacute reduction sufficient to...
Viral vectors are rapidly being developed for a range of applications in research and gene therapy. Prototype foamy virus (PFV) have been described therapy, although their use has mainly restricted to ex vivo stem cell modification. Here we report direct transgene delivery with PFV carrying reporter constructs. In our investigations, systemic vector neonatal mice gave expression the heart, xiphisternum, liver, pancreas, gut, whereas intracranial administration produced brain until animals...
The amygdala, and more precisely its lateral nucleus, is thought to attribute emotional valence external stimuli by generating long-term plasticity changes at long-range projections principal cells. Aversive experience has also been shown modify pre- post-synaptic markers in the suggesting their possible role structural organization of adult amygdala networks. Here, we focused on how maturation cortical thalamic occurs neurons interneurons (LA). We performed dual electrophysiological...
Abstract LGI1 (Leucine-Rich Glioma-Inactivated 1) is a secreted trans-synaptic protein that interacts presynaptically with Kv1.1 potassium channels and ADAM23, postsynaptically, influencing AMPA receptors through direct link the ADAM22 cell adhesion protein. Haploinsufficiency of or autoantibodies directed against are associated human epilepsy, generating hypothesis subacute reduction sufficient to increase network excitability. We tested this in ex vivo hippocampal slices neuronal cultures,...